LOCUS       sequence-76171-e         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Galactose-inducible expression of ER-membrane S. pyogenes SrtA in 
            yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Strijbis K, Spooner E, Ploegh HL
  TITLE     Protein ligation in living cells using sortase.
  JOURNAL   Traffic. 2012 Jun;13(6):780-9. doi: 
            10.1111/j.1600-0854.2012.01345.x. Epub 2012 Mar 23.
  PUBMED    22348280
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1600-0854.2012.01345.x"; journalName: "Traffic"; date: 
            "2012-06"; volume: "13"; issue: "6"; pages: "780-9"
COMMENT     Sequence Label: pRS303-pGAL-HA-TMSec66-SrtA (pKS105)
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        34..475
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    34..151
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     446..469
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             499..525
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 agactcacta tagggcgaat tggagctcta gtacggatta gaagccgccg agcgggtgac
       61 agccctccga aggaagactc tcctccgtgc gtcctcgtct tcaccggtcg cgttcctgaa
      121 acgcagatgt gcctcgcgcc gcactgctcc gaacaataaa gattctacaa tactagcttt
      181 tatggttatg aagaggaaaa attggcagta acctggcccc acaaaccttc aaatgaacga
      241 atcaaattaa caaccatagg atgataatgc gattagtttt ttagccttat ttctggggta
      301 attaatcagc gaagcgatga tttttgatct attaacagat atataaatgc aaaaactgca
      361 taaccacttt aactaatact ttcaacattt tcggtttgta ttacttctta ttcaaatgta
      421 ataaaagtat caacaaaaaa ttgttaatat acctctatac tttaacgtca aggagaaaaa
      481 accccggatt ctagaatgta cccatacgac gtaccagact acgcagagac gaaatcaatc
      541 tccgtttata ccccactcat atatgtcttt attctggtgg tgtcccttgt gatgtttgct
      601 tcaagcggat ccgagccagt tagtacagag agtgtcttgc aagcacaaat ggcggctcag
      661 caacttcctg ttataggggg cattgccata ccagagcttg gcattaattt accaattttt
      721 aaaggtttag gaaatactga gcttatttat ggcgcaggaa c
//
