LOCUS       sequence-76175-e         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Kanamycin resistant; expresses luxAB-DAS+4 under the control of a 
            promoter containing PtetO-4C5G; integrates into the 
            mycobacteriophage att-L5 site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Kim JH, O'Brien KM, Sharma R, Boshoff HI, Rehren G, Chakraborty S, 
            Wallach JB, Monteleone M, Wilson DJ, Aldrich CC, Barry CE 3rd, Rhee 
            KY, Ehrt S, Schnappinger D
  TITLE     A genetic strategy to identify targets for the development of drugs 
            that prevent bacterial persistence.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Nov 4.
  PUBMED    24191058
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Nov 4."
COMMENT     Sequence Label: pGMCK-0X750-luxAB-DAS+4
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 actatgtata ataaagttga agcttgatat cgaattcctg cagcccgggg gatccattta
       61 aatcagagct ttcgcgagct cgagatccgg ctgtttttgt tcctgcaatg accattgctg
      121 aggagttccc atgaaaatca agtcatcgat ttctgtgctc gctgcgatat tttcctgctt
      181 aaccgctgaa agtacgtcca tataaatgcc tctattagtt agcgctatcg cgcgaaaaga
      241 atggtgatat aaggggcatc gctgcccctt cagcatcagt taaacgacta gttattagct
      301 ggcgtccgcg tagttctcgg agtagttctc gtcgttggcg gccgagtggt atttgacgat
      361 gttggcgttt accacatcga taaccgctct ttgctgcgct ttatcttcca tcgactcaaa
      421 agacatcaat aggtccgcgg caccacaaca ctcaatcgca actcgcgctg cctgagtact
      481 ttcttcataa gtaccgatgg cattttctga caacagctct cccatttttt gctcaaagtc
      541 ggtatttgag taagattcac ggacaaactc ttccaaatac acgcgagctt ctgcccttgc
      601 tgcttcacca tctacatttt ggttgaccag cagcgtcagc ttgtgtcgaa cctgactaac
      661 atcgacacca tgtgcctgag caacttcgtg gtacaaaccg gcgtattctt ttctttgagc
      721 gtttgagtcg tcccatctaa acacgagtgg aagccctaac ttagccgccc attcaaccac
      781 ttctttgctc gtcgcattca caaattgcgc aggaccgcct tcagtgaacg cgtgtgggtt
      841 aacggagatt ttaggaaaac tataaaaatc attgtttgga tggcagtacc cagtagtgaa
      901 tgcatcattg atgatcttgt gacactcact gaacaactga aactga
//
