LOCUS       sequence-76178-e         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Kanamycin resistant; expresses luxAB-DAS+4 under the control of a 
            promoter containing PtetO-4C5G; constitutively expresses reverse 
            TetR (tetR38); integrates into the mycobacteriophage att-L5 site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Kim JH, O'Brien KM, Sharma R, Boshoff HI, Rehren G, Chakraborty S, 
            Wallach JB, Monteleone M, Wilson DJ, Aldrich CC, Barry CE 3rd, Rhee 
            KY, Ehrt S, Schnappinger D
  TITLE     A genetic strategy to identify targets for the development of drugs 
            that prevent bacterial persistence.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Nov 4.
  PUBMED    24191058
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Nov 4."
COMMENT     Sequence Label: pGMCK-T38S38-750-luxAB-DAS+4
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgtataata aagttgaagc ttgatatcga attcctgcag cccgggggat ccatttaaat
       61 cagagctttc gcgagctcga gatccggctg tttttgttcc tgcaatgacc attgctgagg
      121 agttcccatg aaaatcaagt catcgatttc tgtgctcgct gcgatatttt cctgcttaac
      181 cgctgaaagt acgtccatat aaatgcctct attagttagc gctatcgcgc gaaaagaatg
      241 gtgatataag gggcatcgct gccccttcag catcagttaa acgactagtt attagctggc
      301 gtccgcgtag ttctcggagt agttctcgtc gttggcggcc gagtggtatt tgacgatgtt
      361 ggcgtttacc acatcgataa ccgctctttg ctgcgcttta tcttccatcg actcaaaaga
      421 catcaatagg tccgcggcac cacaacactc aatcgcaact cgcgctgcct gagtactttc
      481 ttcataagta ccgatggcat tttctgacaa cagctctccc attttttgct caaagtcggt
      541 atttgagtaa gattcacgga caaactcttc caaatacacg cgagcttctg cccttgctgc
      601 ttcaccatct acattttggt tgaccagcag cgtcagcttg tgtcgaacct gactaacatc
      661 gacaccatgt gcctgagcaa cttcgtggta caaaccggcg tattcttttc tttgagcgtt
      721 tgagtcgtcc catctaaaca cgagtggaag ccctaactta gccgcccatt caaccacttc
      781 tttgctcgtc gcattcacaa attgcgcagg accgccttca gtgaacgcgt gtgggttaac
      841 ggagatttta ggaaaactat aaaaatcatt gtttggatgg cagtacccag tagtgaatgc
      901 atcattgatg atcttgtgac actcactgaa caactgaaac tgagaatccg ttgg
//
