LOCUS       sequence-7618-ex        1870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Kaiser SE, Brickner JH, Reilein AR, Fenn TD, Walter P, Brunger AT
  TITLE     Structural basis of FFAT motif-mediated ER targeting.
  JOURNAL   Structure. 2005 Jul . 13(7):1035-45.
  PUBMED    16004875
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Structure. 
            2005 Jul . 13(7):1035-45."
COMMENT     Sequence Label: pGEX-T-rnVAPA-1-219
FEATURES             Location/Qualifiers
     source          1..1870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     534..556
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             583..600
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(1329..1351)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1496..1514)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1582..1686
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaatatgaag agcatttgta tgagcgcgat gaaggtgata aatggcgaaa caaaaagttt
       61 gaattgggtt tggagtttcc caatcttcct tattatattg atggtgatgt taaattaaca
      121 cagtctatgg ccatcatacg ttatatagct gacaagcaca acatgttggg tggttgtcca
      181 aaagagcgtg cagagatttc aatgcttgaa ggagcggttt tggatattag atacggtgtt
      241 tcgagaattg catatagtaa agactttgaa actctcaaag ttgattttct tagcaagcta
      301 cctgaaatgc tgaaaatgtt cgaagatcgt ttatgtcata aaacatattt aaatggtgat
      361 catgtaaccc atcctgactt catgttgtat gacgctcttg atgttgtttt atacatggac
      421 ccaatgtgcc tggatgcgtt cccaaaatta gtttgtttta aaaaacgtat tgaagctatc
      481 ccacaaattg ataagtactt gaaatccagc aagtatatag catggccttt gcagggctgg
      541 caagccacgt ttggtggtgg cgaccatcct ccaaaatcgg atctggttcc gcgtggatcc
      601 catatggcga aacacgagca aatcctggtc ctcgaccctc cttcagacct caaattcaaa
      661 ggccccttca cagatgtagt cactacaaat cttaaattgc agaatccatc tgacagaaaa
      721 gtgtgtttca aagtgaagac cacagcgccc cgccggtact gcgtgcggcc caacagtggg
      781 gttattgacc cagggtcaat cgtgactgtc tcagtaatgc tgcagccctt tgactatgat
      841 ccgaacgaaa agagtaaaca taagttcatg gtacagacaa tttttgctcc accaaacatt
      901 tcagatatgg aagctgtgtg gaaagaggca aaacctgacg aactaatgga ttctaaactg
      961 agatgtgtat ttgagatgcc gaatgaaaac gataagctga acgacatgga acctagcaaa
     1021 gctgtcccac tgaatgcatc caagcaagat ggacccctgc caaaaccaca tagtgtttca
     1081 ctcaatgaca cggagacaag gaaactgatg gaagagtgca agcgacttca gggagaaatg
     1141 atgaagctct cagaagaaaa ccgacacctg agagatgaag gcctacggct cagaaaggta
     1201 gcacattcgg ataaacctgg atccacctca gccgtgtcct tcagagataa tgtcaccagt
     1261 tagctcgagc ggccgcatcg tgactgactg acgatctgcc tcgcgcgttt cggtgatgac
     1321 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     1381 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
     1441 gccatgaccc agtcacgtag cgatagcgga gtgtataatt cttgaagacg aaagggcctc
     1501 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     1561 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     1621 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     1681 aagagtatga gtattcaaca tttccgtgtc gcccttattc cctttttgcg gcattttgcc
     1741 ttcctgtttt gctcacccag aaacgctggt gaaagtaaag atgctgaaga tcagttggtg
     1801 cagcgagtgg gttacatcga ctggatctca cagcggtaga ttcttgagag tttcgcccga
     1861 gaagtttcca
//
