LOCUS       sequence-76180-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Kang JY, Lee HH, Yoon HJ, Kim HS, Suh SW
  TITLE     Overexpression, crystallization and preliminary X-ray 
            crystallographic analysis of phosphopantetheine adenylyltransferase 
            from Enterococcus faecalis.
  JOURNAL   Acta Crystallograph Sect F Struct Biol Cryst Commun. 2006 Nov 1. 
            62(Pt 11):1131-3.
  PUBMED    17077496
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Acta 
            Crystallograph Sect F Struct Biol Cryst Commun. 2006 Nov 1. 62(Pt 
            11):1131-3."
COMMENT     Sequence Label: EF2451_pET21a(+)
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..43)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(546..578)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     RBS             586..608
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    623..647
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(647..666)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(648..666)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(750..769)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gggctttgtt agcagccgga tctcagtggt ggtggtggtg gtgctcgagg ctccagtcat
       61 tcttcttttg ttttaacgca tgataaatat tgggaggaag atagtcagac acatcgcccc
      121 caaaacgtaa aacttctttt agtaaactgg aacttacatg tgcatatggt tcttctgcta
      181 atagaaaaac cgtttcaatt tctggcgcca gatgttgatt cattttagca atgtctttct
      241 cgtactcgta gtctttgacg tttctaatgc cacgaatcaa aaaattagcc cctaatgatt
      301 ttgcactttc cacagttaac tgggtttctt gcataataac acgcacattt ggcatttctt
      361 tagttgcttc ttcaattaaa tattttttct cttctggcgt aaataatgtt tgcttacttg
      421 tatttataaa aacgccaata atgacttcgt caaataactt cgcactgcgt tcaatcaaat
      481 ttaaatgacc atttgtcatc ggatcaaaac ttccgggaaa cagagcaatt ttacgcatgg
      541 atccgaccca tttgctgtcc accagtcatg ctagccatat gtatatctcc ttcttaaagt
      601 taaacaaaat tatttctaga ggggaattgt tatccgctca caattcccct atagtgagtc
      661 gtattaattt cgcgggatcg agatctcgat cctctacgcc ggacgcatcg tggccggcat
      721 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
      781 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
      841 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt
      901 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc
//
