LOCUS       sequence-76181-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Kanamycin resistant; contains qtag28, expresses sspB under a 
            promoter containing PtetO, and constutively expresses single-chain 
            TetR10 (tsc10); integrates into the mycobacteriophage att-Tweety 
            site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Kim JH, O'Brien KM, Sharma R, Boshoff HI, Rehren G, Chakraborty S, 
            Wallach JB, Monteleone M, Wilson DJ, Aldrich CC, Barry CE 3rd, Rhee 
            KY, Ehrt S, Schnappinger D
  TITLE     A genetic strategy to identify targets for the development of drugs 
            that prevent bacterial persistence.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Nov 4.
  PUBMED    24191058
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Nov 4."
COMMENT     Sequence Label: pGMCtKq28-TSC10M1-sspB
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..27
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 agctatcaac tttgtataga aaagttgcag ctggctagcg agtcatgagg tcaccatggt
       61 cattaggagc cgctctcgca cttcagctgc ttctccaagc cgcagatgat cagctccagg
      121 ccgaacaaga aggccggctc ggcgccctgg tggtcgaaca gctcgatggc ctggcgcagc
      181 aacggcggca tgctgtcggt ggtcggggtc tcgcgctcct ccttggcgac ctggtgctcc
      241 tggtcctcca acacgcagcc cagggtgaag tggcccacgg ccgacagggc gtacaacgcg
      301 ttctccagcg agaagccctg ctggcacagg aaggccaact ggttctccag ggtctcgtac
      361 tgcttctcgg tcggccgggt gcccaggtgc accttggcgc cgtcgcggtg gctcagcaag
      421 gcgcagcgga agctcttggc gttgttgcgc aggaagtcct gccagctctc gccctccagc
      481 gggcagaagt gggtgtggtg gcggtccaac atctcgatgg ccagggcgtc gagcagggcc
      541 cgcttgttct tgacgtgcca gtacagggtc ggctgctcga cgcccagctt ctgggccagc
      601 ttgcgggtgg tcaggccctc gatgccgacc tcgttcagca gctccagggc ggagttgatg
      661 accttggact tgtccaggcg ggatctagag ccacctccgc ccgatccgcc acctccagag
      721 cctccaccgc cagaaccgcc tccaccggat ccaccgcctc ctgagtcgcc cgactcacac
      781 ttcaactgct tctccagccc acaaatgatg agttccaaac cgaacaggaa cgcgggctcc
      841 gcaccctgat gatcgaagag ctcgatcgcc tgccggagca gcgggggcat cgaatccgtg
      901 gtgggcgtct cccgctcttc cttcgccacc tgatgttctt gg
//
