LOCUS       sequence-76184-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  inducible (dox) expression of hSox2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  TITLE     McKinnon Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLP-TRE2-hSox2
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      54..148
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(184..219)
                     /codon_start=1
                     /product="6x(His-Asn) affinity tag"
                     /label=6xHN
                     /translation="HNHNHNHNHNHN"
ORIGIN
        1 cagtactgcg atgagtggca gggcggggcg taatttgata tcgagctcgc ttggactcct
       61 gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg
      121 ccgccgggcg ttttttattg gtgagaatcc aagcactagt catgaccagg atctcctagg
      181 ctagttgtga ttatgattat gattatgatt atgattatga ccacttacct gggcccgcgc
      241 gccaaacgaa tggtctagaa agcttggcat gtgtgagagg ggcagtgtgc cgttaatggc
      301 cgtgccgggc accgggccgc tctggtagtg ctgggacatg tgaagtctgc tgggggcggc
      361 gggttccggc acctcggcgc cggggagata catgctgatc atgtcccgga ggtccccggc
      421 ctggcagggc gccctggagt gggaggaaga ggtaaccaca ggggggctgg agctggcctc
      481 ggacttgacc accgaaccca tggagccaag agccatgcca ggggtgccct gctgcgagta
      541 ggacatgctg taggtgggcg agccgttcat gtaggtctgc gagctggtca tggagttgta
      601 ctgcagggcg ctcacgtcgt agcggtgcat gggctgcatc tgcgctgcgc cgtgcgcatt
      661 gaggcccggg tgctgcgggt agcccagctg gtcctgcatc atgctgtagc tgccgttgct
      721 ccagccgttc atgtgcgcgt aactgtccat gcgctggttc acgcccgcgc ccaggccggc
      781 gcccaccccg accccgctcg ccatgctatt gccgccgggg gccagcagcc cgccgggcag
      841 cgtgtactta tccttcttca tgagcgtctt ggttttccgc cggggccggt atttataatc
      901 cggg
//
