LOCUS       sequence-7619-ex        1325 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Kaiser SE, Brickner JH, Reilein AR, Fenn TD, Walter P, Brunger AT
  TITLE     Structural basis of FFAT motif-mediated ER targeting.
  JOURNAL   Structure. 2005 Jul . 13(7):1035-45.
  PUBMED    16004875
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Structure. 
            2005 Jul . 13(7):1035-45."
COMMENT     Sequence Label: pGEX-T-rnVAPA-1-125
FEATURES             Location/Qualifiers
     source          1..1325
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     409..431
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             458..475
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(922..944)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1089..1107)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1175..1279
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tatggccatc atacgttata tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga
       61 gcgtgcagag atttcaatgc ttgaaggagc ggttttggat attagatacg gtgtttcgag
      121 aattgcatat agtaaagact ttgaaactct caaagttgat tttcttagca agctacctga
      181 aatgctgaaa atgttcgaag atcgtttatg tcataaaaca tatttaaatg gtgatcatgt
      241 aacccatcct gacttcatgt tgtatgacgc tcttgatgtt gttttataca tggacccaat
      301 gtgcctggat gcgttcccaa aattagtttg ttttaaaaaa cgtattgaag ctatcccaca
      361 aattgataag tacttgaaat ccagcaagta tatagcatgg cctttgcagg gctggcaagc
      421 cacgtttggt ggtggcgacc atcctccaaa atcggatctg gttccgcgtg gatcccatat
      481 ggcgaaacac gagcaaatcc tggtcctcga ccctccttca gacctcaaat tcaaaggccc
      541 cttcacagat gtagtcacta caaatcttaa attgcagaat ccatcggaca gaaaagtgtg
      601 tttcaaagtg aagaccacag cgccccgccg gtactgcgtg cggcccaaca gtggggttat
      661 tgacccaggg tcaatcgtga ctgtctcagt aatgctgcag ccctttgact atgatccgaa
      721 cgaaaagagt aaacataagt tcatggtaca gacaattttt gctccaccaa acatttcaga
      781 tatggaagct gtgtggaaag aggcaaaacc tgacgaacta atggattcta aactgagatg
      841 tgtatttgag atgtagctcg agcggccgca tcgtgactga ctgacgatct gcctcgcgcg
      901 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
      961 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     1021 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata attcttgaag
     1081 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     1141 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     1201 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     1261 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     1321 tgcgg
//
