LOCUS       sequence-76202-e       10317 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full length GLURP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10317)
  AUTHORS   Zenonos ZA, Rayner JC, Wright GJ
  TITLE     Towards a comprehensive Plasmodium falciparum merozoite cell surface
            and secreted recombinant protein library.
  JOURNAL   Malar J. 2014 Mar 12;13(1):93. doi: 10.1186/1475-2875-13-93.
  PUBMED    24620899
REFERENCE   2  (bases 1 to 10317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2014-03-12"; volume: "13(1):93. doi"; pages: " 
            10.1186/1475-2875-13-93"
COMMENT     Sequence Label: GLURP-bio-His
FEATURES             Location/Qualifiers
     source          1..10317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5481..5498
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5550..5605
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5604..5623)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7841..7859)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7927..8031
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8032..8100,8101..8892)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8250..8269)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9063..9651
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9552..9571
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9805..9822
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9939..9960
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9975..9980,9981..9998,9999..10005)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10013..10029
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10018..10040
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10037..10053
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10037..10053
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcaa
     1201 agacaaacac cgccgagaac cggaacaagc ggatcggcgg acctaagctg agaggcaacg
     1261 tggccagcaa catcaagttc cccagcgaca acaagggcaa gatcatccgg ggcagcaacg
     1321 acaagctgaa caagaacagc gaggacgtgc tggaacagag cgagaagtcc ctggtgtccg
     1381 agaacgtgcc cagcggcctg gacatcgacg acatccccaa agagagcatc ttcatccaag
     1441 aggaccaaga gggccagacc cacagcgagc tgaaccccga gacaagcgag cacagcaagg
     1501 acctgaacaa caacgacgcc aagaacgagg cctccgacat catcagcgtc aacaacaagg
     1561 ccaacaaggt gcagaaccac ttcgagagcc tgagcgacct ggaactgctg gaaaacagcg
     1621 cccaggacaa cctggacaag gacaccatca gcaccgagcc cttcccaaac cagaagcaca
     1681 aggatctgca gcaggatctg aacgacgagc ccctggaacc attcccaacc cagatccaca
     1741 aagactacaa agagaagaac ctgatcaacg aagaggacag cgagccattc ccccggcaga
     1801 aacacaagaa ggtggacaac cacaacgaag aaaagaacgt gttccacgag aacggcgctg
     1861 ccaacggcaa tcagggcagc ctgaagctga agtccttcga cgagcacctg aaggacgaga
     1921 agatcgagaa cgagcctctg gtgcacgaga acctggccat ccccaacgac cccatcgagc
     1981 agatcctgaa ccagcccgag caagagacaa atatccaaga gcagctgtac aacgagaagc
     2041 agaacgtgga agagaaacag aacagccaga tccccagcct ggacctgaaa gagcccacca
     2101 acgaggacat cctgcccaat cacaaccccc tcgagaacat caagcagagc gagtccgaga
     2161 tcaaccacgt gcaggaccac gccctgccta aagagaatat catcgacaag ctcgacaacc
     2221 agaaagagca catcgaccag agccagcaca acatcaacgt gctgcaagag aacaatatca
     2281 acaaccacca gctcgagccc caagagaagc ccaacatcga gagcttcgag cccaagaaca
     2341 tcgacagcga gatcatcctg cctgagaacg tcgagacaga agagatcatt gacgacgtgc
     2401 caagccccaa gcactccaac cacgagacat tcgaggaaga aaccagcgag agcgagcacg
     2461 aggaagccgt gtctgagaag aacgcccacg agactgtgga acatgaggaa acagtgtccc
     2521 aagagagcaa ccctgagaag gccgacaacg acggcaatgt ggcccagaac agcaacaatg
     2581 agctgaacga gaatgagttc gtcgagagcg aaaagtctga gcacgagcca gccgagaacg
     2641 aagagagcag cctggaagag ggacaccacg aagagatcgt gcccgagcag aacaacgagg
     2701 aaagcggcga gagcaagctg gtggacaatg acgagggcgg cttcgaagag gcccaccatg
     2761 agaacttcgc cagcgaggtg tccaactccg aactgaatga gaacgagttt gtggaaagcg
     2821 acaagagcgt gaccgagccc gccgagcatg aagaagtggt gtctgaggaa agcaacccag
     2881 agcctgccga aaacgaggaa tccagcattg aggaagccca tcaagaggaa attgtccctg
     2941 agcagaatga cgaagagtct ggcgagtccg gcctggtgga taacgaggaa ggcgacttcg
     3001 aggaacccaa tcacgaagag ttcgaacctg accagaacga cgccgagctg tccgaaaatg
     3061 aactggtgga atccgagaaa tccgtgtccg aacctgccga gcacgtggaa attgtgtcag
     3121 agaagtctgt gtccgagcca gctgaacacg tcgagatcgt gtctgaaaag tccacctctg
     3181 agcctgctga gcatgttgaa tctgtgtctg agcagtccaa caacgagccc agcgagaaga
     3241 aagacggccc cgtgcctagc aagcccttcg aagaaatcga gaaagtggac gtgcagccca
     3301 agatcgtgga cctgcagatc atcgagccta acttcgtgga cagccagccc aacccccaag
     3361 agccagtgga acccagcttc gtgaagattg agaaggtgcc ctccgaggaa aacaagcacg
     3421 ccagcgtgga ccccgaagtg aaagaaaaag aaaacgtggc cgaggtggtc gaggaaaagc
     3481 agaactccca agaaagcgtg gaagaaatcc ccgtgaacga ggacgaattc gaggatgtgc
     3541 acaccgagca gctggacctg gaccacaaga ccgtggatcc cgagattgtg gaagttgaag
     3601 agatcccctc cgagttgcac gaaaatgagg tggcccaccc cgaaatcgtg gaaatcgaag
     3661 aggtgttccc cgagcccaac cagaacaatg agttccaaga gatcaacgag gatgacaagt
     3721 ccgcccacat ccagcacgag atcgtcgagg ttgaggaaat tctgcccgag gacgacaaaa
     3781 atgagaaggt cgagcacgaa atcgttgagg tggaagagat cctgccagag gacaagaacg
     3841 agaaagtgca gcatgagatc gttgaagtcg aagaaatact ccccgaggat aagaacgaaa
     3901 aggtcgaaca cgagatagtg gaagtcgagg aaatccttcc tgaggacaag aatgagaagg
     3961 gccaacacga gattgtcgag gtcgaagaga ttctccctga agataagaat gagaaagttc
     4021 agcacgaaat tgtcgaagtg gaagaaatat tgcctgagga caaaaacgaa aaggggcagc
     4081 acgaaatcgt ggaagttgag gaaatcttgc ccgaagataa gaacgagaag gttgagcacg
     4141 agatcgtcga agttgaagag atcctgcctg aggacaaaaa tgagaaagga cagcatgaga
     4201 tagtcgaggt ggaagagatc cttcccgagg acgataagaa cgagaaaggc caacacgaaa
     4261 tcgtggaagt cgaagagatt ctgcccgaga tcgtcgagat cgaagaagtg cccagccaga
     4321 cgaacaacaa tgagaacatc gaaaccatca agcccgagga aaagaaaaac gagttcagcg
     4381 tcgtcgaaga gaaggccatc cctcaagagc ccgtggtgcc caccctgaac gaaaacgaga
     4441 atgtggcccc caagcctagc gagggcgagt ccaccaagcc cgacatcgtg cagatcaaga
     4501 ttgtgcaaga aaacaaaccc aacaagaaag aaacccccgt cgtggacggc cccaaacacg
     4561 tggaacagaa cattcaagag gatgataacg acgaggaaga tgacgacgac attgatttcg
     4621 agggcctgtc ccggaaggac gacgagaagg actccagcaa caaaaacaag aagaagtcca
     4681 gcttcatcac ctacatctcc accaagaagt tcaagaaggt ttcccagacc atcgtgtccg
     4741 tgatgatcaa cgcctacgac ggcgtgatcc aggtggtgtc caccatcaag ggaatcgcca
     4801 aggatatcgt gatcttcttc cagaacattg gcgcgccgtc gacctccatc acggcctata
     4861 agagtgaggg ggagtcagcg gagttctcct tcccactcaa ccttggagag gaaagcctgc
     4921 agggagagtt gagatggaag gcagagaagg ctccttcttc ccagtcctgg atcaccttct
     4981 ccctaaagaa ccaaaaggtg tctgtgcaga agtctactag caaccccaag ttccagctgt
     5041 ccgaaacgct cccactcacc cttcagatac cccaggtctc ccttcagttt gctggttctg
     5101 gcaacctgac cctgactctg gacagaggga tactgtatca ggaagtgaac ctggtggtga
     5161 tgaaagtgac tcagcccgac agcaacactt tgacctgtga ggtgatggga cccacctcac
     5221 ccaagatgag actgatcttg aagcaggaga atcaggaggc cagggtctcc aggcaggaga
     5281 aagtgattca agtgcaggcc cctgaagcag gggtgtggca atgtctactg agtgaaggtg
     5341 aagaggtcaa gatggactcc aagatccagg ttttatccaa agggttgaat tccggcagcc
     5401 tgcaccacat cctggacgcc cagaagatgc tgtggaacca cagggacagg aacctgcctc
     5461 ctctggcccc tctgggcccc caccaccatc accaccattg agtctagaca cgtgattaat
     5521 taaggatccc ccgacctcga cctctggcta ataaaggaaa tttattttca ttgcaatagt
     5581 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttggt
     5641 cgagatccct cggagatctc tagctagagg atcgatcccc gccccggacg aactaaacct
     5701 gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt cagttggttg
     5761 gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc aaacacaaag
     5821 gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc caacactgag
     5881 tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca ttgcctttat
     5941 gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct cccaggggcc
     6001 caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta gctaccgata
     6061 agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt taaccctaaa
     6121 cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc taattcaata
     6181 gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa agggtcctaa
     6241 ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg gggtcaggat
     6301 tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc aaggagcggg
     6361 cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag ctaatagaat
     6421 aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag gtttcaggtg
     6481 acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct atgacaccaa
     6541 tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa cattctgaat
     6601 atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga tgtccatctc
     6661 acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac tggggttatt
     6721 aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact ctatttgtaa
     6781 caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct ctaacacccc
     6841 cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag tggccactct
     6901 tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc ctgcggtttt
     6961 ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac cactgcggtc
     7021 aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat aagtaggtgg
     7081 gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac acacttgcgc
     7141 ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga gagcacggtg
     7201 ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat atgctatcct
     7261 aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     7321 aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct
     7381 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     7441 aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat atgctatcct
     7501 aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc
     7561 tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc
     7621 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt
     7681 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     7741 tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctcatg
     7801 ataagctgtc aaacatgaga attttcttga agacgaaagg gcctcgtgat acgcctattt
     7861 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     7921 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     7981 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     8041 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     8101 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     8161 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     8221 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     8281 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     8341 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     8401 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     8461 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     8521 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     8581 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     8641 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     8701 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     8761 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     8821 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     8881 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     8941 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     9001 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     9061 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     9121 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     9181 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     9241 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     9301 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     9361 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     9421 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     9481 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     9541 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     9601 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     9661 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     9721 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     9781 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     9841 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     9901 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     9961 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
    10021 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct ctagctagag
    10081 gtcgaccaat tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat
    10141 tgctgcaggc gcagaactgg taggtatgga agatctatac attgaatcaa tattggcaat
    10201 tagccatatt agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata
    10261 cgttgtatct atatcataat atgtacattt atattggctc atgtccaata tgaccgc
//
