LOCUS       sequence-76204-e        8322 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full length MSP3.6 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8322)
  AUTHORS   Zenonos ZA, Rayner JC, Wright GJ
  TITLE     Towards a comprehensive Plasmodium falciparum merozoite cell surface
            and secreted recombinant protein library.
  JOURNAL   Malar J. 2014 Mar 12;13(1):93. doi: 10.1186/1475-2875-13-93.
  PUBMED    24620899
REFERENCE   2  (bases 1 to 8322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2014-03-12"; volume: "13(1):93. doi"; pages: " 
            10.1186/1475-2875-13-93"
COMMENT     Sequence Label: MSP3.6-bio-His
FEATURES             Location/Qualifiers
     source          1..8322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3486..3503
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3555..3610
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3609..3628)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5846..5864)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5932..6036
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6037..6105,6106..6897)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6255..6274)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7068..7656
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7557..7576
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7810..7827
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7944..7965
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7980..7985,7986..8003,8004..8010)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8018..8034
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8023..8045
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8042..8058
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8042..8058
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcaa
     1201 atggcgccct gagcgagaat atcgagagcg ccgaggaaat cgacgccctg aaaacaaacc
     1261 tgcggaacgg ctacctgaac aacgcctact tcaacgagga aaacaacaac ctgaacatcg
     1321 agaacgagat caacaacgcc aactacaatg aagtgaccga ggaaacgaaa gaggaactgt
     1381 acgacatcaa tgagaatatc ttccccgact acttcttcct ggacatcttc accgagaaca
     1441 aagagcagaa aaacgaagag gtgcccatga agatcgaggt ggtcaacgac ggcgaggaag
     1501 tgaaaaccga gtacgtgtcc gagaagaacg aggaagtcga gaacaaggcc gagacagaga
     1561 tcggggagga actgaccgag aaggtggacg agaaagtgcc tgaagaggtg gccgaggaac
     1621 tggtggaaaa agtggatgag gaagtggcag aagaactggt cgagaaagtc gacgaaaagg
     1681 tcgcagaaga ggtcgaccag aaagttgatg aagaagtcac agaggaactc attgaaaagg
     1741 tcgacgagga agttaccgaa gaactcatcg agaaggttga cgaagaagtg gctgaggaat
     1801 tgatagaaaa ggtggacgaa gaggtcgcag aggaacttat cgagaaagtg gccgacgagc
     1861 tgattgagaa agtggacgag gaagtcgccg aggaactcat agaaaaagtg gctgatgagc
     1921 ttgtcgaaaa agtcgctgaa gaactcgttg agaaagtgga tgaagaggtt gccgaagaat
     1981 tggttgagaa ggttgacgag aaggtggccg aagaagtcga tcaaaaagtt gacgaggaag
     2041 ttactgagga actgattgag aaagtcgatg aagaagtgac ggaagaactt atcgaaaaag
     2101 ttgatgaaga ggtcgccgag gaactgatcg aaaaggtcga cgaagaggtg gccgaagaac
     2161 tcattgaaaa agtcgccgac gagctggtgg aaaaggtggc agaggaactt gttgagaagg
     2221 tggacgaaca agtggcagag gaactcgtgg aaaaggttga cgagcaggtt gccgaggaac
     2281 tggtcgagaa agttgatgaa caggtggtgg aagaggttgc agaagaggtc gccgaagagg
     2341 ttgtggaaga gggcgagaag gttccggaag aagtggccga agaagttgcc gaagaagtcg
     2401 ctgaagaggt tgccgaggaa gtggccgaag aacttgtgga aaaagtggac gaagaagtcg
     2461 ctgagaaggt cgtggaagag gaaggcgaga aggtccccga agaagtcgtg gaagaagttg
     2521 acgaagaggt tgcagagaag gtggtcgagg aagaggggga gaaagttttg gaagaagtca
     2581 tcgaagaggt cgtggaagaa gtggctgaag aagtcgcaga gaaagttgtt gaggaacaag
     2641 gcgaaaaagt gaacaaaaac gacctcaacg acgccagcag cgaagagatc aaggacagct
     2701 ccgacttcaa agagagccac gaagaactgt tcaaggtgtt cctcgagctg atcaacaaga
     2761 acgatctggt caaagagaac ctgaagaaga tcaccaacaa tctgaacgag atgcacctga
     2821 gcaccctgta ccctggcgcg ccgtcgacct ccatcacggc ctataagagt gagggggagt
     2881 cagcggagtt ctccttccca ctcaaccttg gagaggaaag cctgcaggga gagttgagat
     2941 ggaaggcaga gaaggctcct tcttcccagt cctggatcac cttctcccta aagaaccaaa
     3001 aggtgtctgt gcagaagtct actagcaacc ccaagttcca gctgtccgaa acgctcccac
     3061 tcacccttca gataccccag gtctcccttc agtttgctgg ttctggcaac ctgaccctga
     3121 ctctggacag agggatactg tatcaggaag tgaacctggt ggtgatgaaa gtgactcagc
     3181 ccgacagcaa cactttgacc tgtgaggtga tgggacccac ctcacccaag atgagactga
     3241 tcttgaagca ggagaatcag gaggccaggg tctccaggca ggagaaagtg attcaagtgc
     3301 aggcccctga agcaggggtg tggcaatgtc tactgagtga aggtgaagag gtcaagatgg
     3361 actccaagat ccaggtttta tccaaagggt tgaattccgg cagcctgcac cacatcctgg
     3421 acgcccagaa gatgctgtgg aaccacaggg acaggaacct gcctcctctg gcccctctgg
     3481 gcccccacca ccatcaccac cattgagtct agacacgtga ttaattaagg atcccccgac
     3541 ctcgacctct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     3601 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttggtcgaga tccctcggag
     3661 atctctagct agaggatcga tccccgcccc ggacgaacta aacctgacta cgacatctct
     3721 gccccttctt cgcggggcag tgcatgtaat cccttcagtt ggttggtaca acttgccaac
     3781 tgggccctgt tccacatgtg acacgggggg ggaccaaaca caaaggggtt ctctgactgt
     3841 agttgacatc cttataaatg gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg
     3901 agcagacttt gcagtctgtg gactgcaaca caacattgcc tttatgtgta actcttggct
     3961 gaagctctta caccaatgct gggggacatg tacctcccag gggcccagga agactacggg
     4021 aggctacacc aacgtcaatc agaggggcct gtgtagctac cgataagcgg accctcaaga
     4081 gggcattagc aatagtgttt ataaggcccc cttgttaacc ctaaacgggt agcatatgct
     4141 tcccgggtag tagtatatac tatccagact aaccctaatt caatagcata tgttacccaa
     4201 cgggaagcat atgctatcga attagggtta gtaaaagggt cctaaggaac agcgatatct
     4261 cccaccccat gagctgtcac ggttttattt acatggggtc aggattccac gagggtagtg
     4321 aaccatttta gtcacaaggg cagtggctga agatcaagga gcgggcagtg aactctcctg
     4381 aatcttcgcc tgcttcttca ttctccttcg tttagctaat agaataactg ctgagttgtg
     4441 aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa
     4501 aatttggacg gggggttcag tggtggcatt gtgctatgac accaatataa ccctcacaaa
     4561 ccccttgggc aataaatact agtgtaggaa tgaaacattc tgaatatctt taacaataga
     4621 aatccatggg gtggggacaa gccgtaaaga ctggatgtcc atctcacacg aatttatggc
     4681 tatgggcaac acataatcct agtgcaatat gatactgggg ttattaagat gtgtcccagg
     4741 cagggaccaa gacaggtgaa ccatgttgtt acactctatt tgtaacaagg ggaaagagag
     4801 tggacgccga cagcagcgga ctccactggt tgtctctaac acccccgaaa attaaacggg
     4861 gctccacgcc aatggggccc ataaacaaag acaagtggcc actctttttt ttgaaattgt
     4921 ggagtggggg cacgcgtcag cccccacacg ccgccctgcg gttttggact gtaaaataag
     4981 ggtgtaataa cttggctgat tgtaaccccg ctaaccactg cggtcaaacc acttgcccac
     5041 aaaaccacta atggcacccc ggggaatacc tgcataagta ggtgggcggg ccaagatagg
     5101 ggcgcgattg ctgcgatctg gaggacaaat tacacacact tgcgcctgag cgccaagcac
     5161 agggttgttg gtcctcatat tcacgaggtc gctgagagca cggtgggcta atgttgccat
     5221 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
     5281 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     5341 agtatatgct atcctaattt atatctgggt agcataggct atcctaatct atatctgggt
     5401 agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
     5461 agcatatgct atcctaatag agattagggt agtatatgct atcctaattt atatctgggt
     5521 agcatatact acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca
     5581 tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca
     5641 tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca
     5701 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     5761 tatgctatcc taatctgtat ccgggtagca tatgctatcc tcatgataag ctgtcaaaca
     5821 tgagaatttt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     5881 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     5941 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     6001 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     6061 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     6121 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     6181 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     6241 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     6301 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     6361 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     6421 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     6481 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     6541 gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg
     6601 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     6661 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     6721 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     6781 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     6841 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6901 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6961 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     7021 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     7081 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     7141 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     7201 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     7261 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     7321 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     7381 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     7441 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     7501 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     7561 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     7621 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     7681 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     7741 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     7801 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     7861 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     7921 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     7981 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     8041 acaggaaaca gctatgacca tgattacgcc aagctctagc tagaggtcga ccaattctca
     8101 tgtttgacag cttatcatcg cagatccggg caacgttgtt gccattgctg caggcgcaga
     8161 actggtaggt atggaagatc tatacattga atcaatattg gcaattagcc atattagtca
     8221 ttggttatat agcataaatc aatattggct attggccatt gcatacgttg tatctatatc
     8281 ataatatgta catttatatt ggctcatgtc caatatgacc gc
//
