LOCUS       sequence-76205-e        8001 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full length MSRP5 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8001)
  AUTHORS   Zenonos ZA, Rayner JC, Wright GJ
  TITLE     Towards a comprehensive Plasmodium falciparum merozoite cell surface
            and secreted recombinant protein library.
  JOURNAL   Malar J. 2014 Mar 12;13(1):93. doi: 10.1186/1475-2875-13-93.
  PUBMED    24620899
REFERENCE   2  (bases 1 to 8001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2014-03-12"; volume: "13(1):93. doi"; pages: " 
            10.1186/1475-2875-13-93"
COMMENT     Sequence Label: MSRP5-bio-His
FEATURES             Location/Qualifiers
     source          1..8001
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3165..3182
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3234..3289
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3288..3307)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5525..5543)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5611..5715
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5716..5784,5785..6576)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5934..5953)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6747..7335
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7236..7255
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7489..7506
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7623..7644
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7659..7664,7665..7682,7683..7689)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7697..7713
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7702..7724
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7721..7737
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7721..7737
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcaa
     1201 acaagcccac cagaaatatc gccgtgaagt ccaacaagga caaggacgag ctgaacaaca
     1261 tcaaagagaa gctggacctg atcaacaacg ccatcaagga caaagtgatc gagaacttca
     1321 aagaggacat cgagctgctg aagaagaagg tcgacgacct ggaaaagcgg aagtccgaca
     1381 acaccctggg caagcggcag aagaaagagg acgacgacga cgaggaagag acagacgagg
     1441 acgatgatga ggacagcgac gaggatgacg aagaacaaga ggaactgaac gtcgagccca
     1501 aagagcgcga ggacgagcaa gaggaaacag acgacgagca gaaagaaacc gaggatgaac
     1561 agaaagagac agaggacgag caaaaagaga ctgaggacga acaaaaagaa accgaagatg
     1621 agcaaaaaga aacggaagat gagcagaaag agagcgacga cgaacagaaa gaaactgagg
     1681 acgagcagaa agaaacagag gacgaggcca gcgaagagta cagcgacaac gaagaggacg
     1741 atgaggaaga tgaagaggac gacgaagagg aaaacgaaga gaacaacgac aatgaggcca
     1801 acgaggaaaa tgaggataac gacgagaacg aggacaacga tgagaatgag gaagagatcg
     1861 aagtcaccga cgtggaattc gtgggccaga gcaccaacaa gaacgtgcgc aacaacatga
     1921 tccggaacag caacaaggat atcaagagca gcagccagaa cagcgccatt aaggcccaga
     1981 actccgccac caagatcggc aacaccccca ccaagctgag cacccagaac accaagagca
     2041 acagcgcctc caaccagctg atcacacagc tgcagagcga gaagtccagc agcaaggtgg
     2101 acaacaacaa aaacaatgcc aacgagatca agtacatgga caagctgtgc gacgacgtgc
     2161 tgaccgaact caaagaaaag gacaacgtcg acaacaatat gaaccacgcc aagtacaaca
     2221 acctcaagaa agagttcagc accttcacca tgaaccagaa cgagtgcgac ctcatcaaga
     2281 agctgatcat caccttcagc caagagaacg tggaaatgaa gcgggactct atcaaagaaa
     2341 tcttcctgaa ggccctggac gacaagaaat accgcgaggt gttcaagaac tttatgtacg
     2401 gcgtgtacag ctacgccaag cggcacaact acctggatat cgagaagatg gaaaagaacg
     2461 agcgggccta taagaagctg ttcgagaaca ccctgaacct gctggacacc attggcgcgc
     2521 cgtcgacctc catcacggcc tataagagtg agggggagtc agcggagttc tccttcccac
     2581 tcaaccttgg agaggaaagc ctgcagggag agttgagatg gaaggcagag aaggctcctt
     2641 cttcccagtc ctggatcacc ttctccctaa agaaccaaaa ggtgtctgtg cagaagtcta
     2701 ctagcaaccc caagttccag ctgtccgaaa cgctcccact cacccttcag ataccccagg
     2761 tctcccttca gtttgctggt tctggcaacc tgaccctgac tctggacaga gggatactgt
     2821 atcaggaagt gaacctggtg gtgatgaaag tgactcagcc cgacagcaac actttgacct
     2881 gtgaggtgat gggacccacc tcacccaaga tgagactgat cttgaagcag gagaatcagg
     2941 aggccagggt ctccaggcag gagaaagtga ttcaagtgca ggcccctgaa gcaggggtgt
     3001 ggcaatgtct actgagtgaa ggtgaagagg tcaagatgga ctccaagatc caggttttat
     3061 ccaaagggtt gaattccggc agcctgcacc acatcctgga cgcccagaag atgctgtgga
     3121 accacaggga caggaacctg cctcctctgg cccctctggg cccccaccac catcaccacc
     3181 attgagtcta gacacgtgat taattaagga tcccccgacc tcgacctctg gctaataaag
     3241 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     3301 atatgggagg gcaaatcatt tggtcgagat ccctcggaga tctctagcta gaggatcgat
     3361 ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt
     3421 gcatgtaatc ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga
     3481 cacggggggg gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg
     3541 atgtgcacat ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg
     3601 actgcaacac aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg
     3661 ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca
     3721 gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta
     3781 taaggccccc ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact
     3841 atccagacta accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa
     3901 ttagggttag taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg
     3961 gttttattta catggggtca ggattccacg agggtagtga accattttag tcacaagggc
     4021 agtggctgaa gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat
     4081 tctccttcgt ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg
     4141 tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt
     4201 ggtggcattg tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta
     4261 gtgtaggaat gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag
     4321 ccgtaaagac tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta
     4381 gtgcaatatg atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac
     4441 catgttgtta cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac
     4501 tccactggtt gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca
     4561 taaacaaaga caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc
     4621 ccccacacgc cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt
     4681 gtaaccccgc taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg
     4741 gggaatacct gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg
     4801 aggacaaatt acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt
     4861 cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa
     4921 tatctggata gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta
     4981 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta
     5041 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     5101 tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga
     5161 gattagggta gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc
     5221 tggatagcat atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     5281 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     5341 tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc
     5401 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc
     5461 cgggtagcat atgctatcct catgataagc tgtcaaacat gagaattttc ttgaagacga
     5521 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     5581 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5641 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     5701 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5761 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     5821 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5881 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     5941 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     6001 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     6061 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     6121 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     6181 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     6241 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
     6301 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6361 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6421 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6481 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6541 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     6601 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     6661 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6721 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6781 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6841 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     6901 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6961 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     7021 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     7081 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     7141 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7201 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7261 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7321 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     7381 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     7441 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     7501 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     7561 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     7621 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     7681 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     7741 gattacgcca agctctagct agaggtcgac caattctcat gtttgacagc ttatcatcgc
     7801 agatccgggc aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tggaagatct
     7861 atacattgaa tcaatattgg caattagcca tattagtcat tggttatata gcataaatca
     7921 atattggcta ttggccattg catacgttgt atctatatca taatatgtac atttatattg
     7981 gctcatgtcc aatatgaccg c
//
