LOCUS       sequence-76206-e        7551 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full length MSRP4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7551)
  AUTHORS   Zenonos ZA, Rayner JC, Wright GJ
  TITLE     Towards a comprehensive Plasmodium falciparum merozoite cell surface
            and secreted recombinant protein library.
  JOURNAL   Malar J. 2014 Mar 12;13(1):93. doi: 10.1186/1475-2875-13-93.
  PUBMED    24620899
REFERENCE   2  (bases 1 to 7551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2014-03-12"; volume: "13(1):93. doi"; pages: " 
            10.1186/1475-2875-13-93"
COMMENT     Sequence Label: MSRP4-bio-His
FEATURES             Location/Qualifiers
     source          1..7551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2715..2732
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2784..2839
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2838..2857)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5075..5093)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5161..5265
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5266..5334,5335..6126)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5484..5503)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6297..6885
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6786..6805
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7039..7056
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7173..7194
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7209..7214,7215..7232,7233..7239)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7247..7263
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7252..7274
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7271..7287
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7271..7287
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcag
     1201 acaagaacac cttccccaaa gagatcatcg agtacgagaa ggacctggaa ctggccaaga
     1261 tcaaagagaa gctgcagaac ctgaacgaca tcatcgtgga caagctgatc gagagcttca
     1321 aggacaacgt ggacgtgctg aaagtgatca tccaggaact cgagaaagaa aaagagaaag
     1381 agaacaagct ggccaagaag aagcgcgaga tcagcaacaa cgacttcctg aacaacaaga
     1441 acagcaacaa gatcaagggc cagggcttct tcaccagcac ctggaagcag attaagggca
     1501 acagcgagga aaacagcgag aaccagcaga agtccagcaa gagccagagc ctgcagagca
     1561 acgagcccaa caagctgaac caggaaaaca ttgcccccgt ccagaccacc atcaccaaca
     1621 ccaatgtgga caacaccatc agcgtgatca agtacctgga caacgcctac gacgaggtcc
     1681 tgaaagagat gaacctggcc aaaaactccg acaacgagaa gtaccggtcc cgcttcgaca
     1741 acttcaagca gggcttcgag aacctgatcc tgaaccagaa tgagtacgag ctgatcaagc
     1801 ggctgatcct ggcattcagc aaccaggaag agagcggcac caacaagaaa aaccacatcg
     1861 tgaacatgct gaagaaggcc ctcgaggaag agaagttcag cgacgagttc aagaacttca
     1921 tctacggcat ctacgcctac gccaagaagc acaactacct gagactgatc gacagcaacc
     1981 gggacatcta caagaacgtg ttcgagaacg ccgccaacct gctggacacc ctgcagatga
     2041 agctgaagcg ggtgccatct cagggcgcgc cgtcgacctc catcacggcc tataagagtg
     2101 agggggagtc agcggagttc tccttcccac tcaaccttgg agaggaaagc ctgcagggag
     2161 agttgagatg gaaggcagag aaggctcctt cttcccagtc ctggatcacc ttctccctaa
     2221 agaaccaaaa ggtgtctgtg cagaagtcta ctagcaaccc caagttccag ctgtccgaaa
     2281 cgctcccact cacccttcag ataccccagg tctcccttca gtttgctggt tctggcaacc
     2341 tgaccctgac tctggacaga gggatactgt atcaggaagt gaacctggtg gtgatgaaag
     2401 tgactcagcc cgacagcaac actttgacct gtgaggtgat gggacccacc tcacccaaga
     2461 tgagactgat cttgaagcag gagaatcagg aggccagggt ctccaggcag gagaaagtga
     2521 ttcaagtgca ggcccctgaa gcaggggtgt ggcaatgtct actgagtgaa ggtgaagagg
     2581 tcaagatgga ctccaagatc caggttttat ccaaagggtt gaattccggc agcctgcacc
     2641 acatcctgga cgcccagaag atgctgtgga accacaggga caggaacctg cctcctctgg
     2701 cccctctggg cccccaccac catcaccacc attgagtcta gacacgtgat taattaagga
     2761 tcccccgacc tcgacctctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     2821 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt tggtcgagat
     2881 ccctcggaga tctctagcta gaggatcgat ccccgccccg gacgaactaa acctgactac
     2941 gacatctctg ccccttcttc gcggggcagt gcatgtaatc ccttcagttg gttggtacaa
     3001 cttgccaact gggccctgtt ccacatgtga cacggggggg gaccaaacac aaaggggttc
     3061 tctgactgta gttgacatcc ttataaatgg atgtgcacat ttgccaacac tgagtggctt
     3121 tcatcctgga gcagactttg cagtctgtgg actgcaacac aacattgcct ttatgtgtaa
     3181 ctcttggctg aagctcttac accaatgctg ggggacatgt acctcccagg ggcccaggaa
     3241 gactacggga ggctacacca acgtcaatca gaggggcctg tgtagctacc gataagcgga
     3301 ccctcaagag ggcattagca atagtgttta taaggccccc ttgttaaccc taaacgggta
     3361 gcatatgctt cccgggtagt agtatatact atccagacta accctaattc aatagcatat
     3421 gttacccaac gggaagcata tgctatcgaa ttagggttag taaaagggtc ctaaggaaca
     3481 gcgatatctc ccaccccatg agctgtcacg gttttattta catggggtca ggattccacg
     3541 agggtagtga accattttag tcacaagggc agtggctgaa gatcaaggag cgggcagtga
     3601 actctcctga atcttcgcct gcttcttcat tctccttcgt ttagctaata gaataactgc
     3661 tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc
     3721 ccagaataaa atttggacgg ggggttcagt ggtggcattg tgctatgaca ccaatataac
     3781 cctcacaaac cccttgggca ataaatacta gtgtaggaat gaaacattct gaatatcttt
     3841 aacaatagaa atccatgggg tggggacaag ccgtaaagac tggatgtcca tctcacacga
     3901 atttatggct atgggcaaca cataatccta gtgcaatatg atactggggt tattaagatg
     3961 tgtcccaggc agggaccaag acaggtgaac catgttgtta cactctattt gtaacaaggg
     4021 gaaagagagt ggacgccgac agcagcggac tccactggtt gtctctaaca cccccgaaaa
     4081 ttaaacgggg ctccacgcca atggggccca taaacaaaga caagtggcca ctcttttttt
     4141 tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg ttttggactg
     4201 taaaataagg gtgtaataac ttggctgatt gtaaccccgc taaccactgc ggtcaaacca
     4261 cttgcccaca aaaccactaa tggcaccccg gggaatacct gcataagtag gtgggcgggc
     4321 caagataggg gcgcgattgc tgcgatctgg aggacaaatt acacacactt gcgcctgagc
     4381 gccaagcaca gggttgttgg tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa
     4441 tgttgccatg ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta
     4501 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     4561 tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta
     4621 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg
     4681 tatccgggta gcatatgcta tcctaataga gattagggta gtatatgcta tcctaattta
     4741 tatctgggta gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc
     4801 tgggtagcat atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc
     4861 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc
     4921 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     4981 tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct catgataagc
     5041 tgtcaaacat gagaattttc ttgaagacga aagggcctcg tgatacgcct atttttatag
     5101 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     5161 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5221 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5281 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5341 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5401 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5461 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     5521 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5581 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     5641 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     5701 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5761 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca
     5821 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5881 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     5941 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6001 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6061 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6121 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6181 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6241 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6301 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6361 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6421 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     6481 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6541 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6601 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6661 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6721 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6781 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6841 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6901 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     6961 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     7021 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     7081 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     7141 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     7201 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     7261 caatttcaca caggaaacag ctatgaccat gattacgcca agctctagct agaggtcgac
     7321 caattctcat gtttgacagc ttatcatcgc agatccgggc aacgttgttg ccattgctgc
     7381 aggcgcagaa ctggtaggta tggaagatct atacattgaa tcaatattgg caattagcca
     7441 tattagtcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt
     7501 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg c
//
