LOCUS       sequence-76207-e        8340 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full length MSP8 
            ectodomain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8340)
  AUTHORS   Zenonos ZA, Rayner JC, Wright GJ
  TITLE     Towards a comprehensive Plasmodium falciparum merozoite cell surface
            and secreted recombinant protein library.
  JOURNAL   Malar J. 2014 Mar 12;13(1):93. doi: 10.1186/1475-2875-13-93.
  PUBMED    24620899
REFERENCE   2  (bases 1 to 8340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2014-03-12"; volume: "13(1):93. doi"; pages: " 
            10.1186/1475-2875-13-93"
COMMENT     Sequence Label: MSP8-bio-His
FEATURES             Location/Qualifiers
     source          1..8340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3504..3521
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3573..3628
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3627..3646)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5864..5882)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5950..6054
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6055..6123,6124..6915)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6273..6292)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7086..7674
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7575..7594
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7828..7845
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7962..7983
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7998..8003,8004..8021,8022..8028)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8036..8052
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8041..8063
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8060..8076
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8060..8076
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcag
     1201 aaaatggcgc caccaacatc gagaacaacc ccggcaacaa cggcaacatg ggccccagcg
     1261 gccccaagga caaggataag aatatcgaga aggacgtgaa ccacaatatg gccatgaaca
     1321 acaacaacaa caataacaat aacaacaaca acaatgataa caacaacaat atcaacaaca
     1381 acaacaataa caacatcaac aacaacaaca ataacaacaa caacaacggc aacggcttca
     1441 gcaacttctt caacaagctg ttcggcaaga agaaggacaa caagaaagag ggcgaggaaa
     1501 agaacgaaga ggacctgaac tccaacaaga acatcgagtc caacaagggc agcgccgtga
     1561 ccagcaacgt gggcgacaca aacaacgacg ccaaggcccg ggacaacaac aacaacgacg
     1621 acaacgatga caacgacgag aatgacgata acgatgataa tgatgacatc gatgagatcg
     1681 acgagcggga cgacaacgac gataacggcg acgacgatga cgacaatgac gacgacgacg
     1741 acaacaacga taacaacaac aagaacaacg ccaacaatct ggccgatacc aagaaagaag
     1801 gggagaagat cgacctgggc gtgcagaaca agaagcagaa catcttcagc accaacaaca
     1861 aaggcctgaa caagtacaac atcgacaatg agctgaaaga ggtggacgcc ctgctgaaga
     1921 acgacaacta catcctcaac aagtaccacg tcagcttttt caacaacttc gaggaagata
     1981 cctacaacaa aaagaagttc atccggccct acgacctgtc tctgctgaag tccatcctga
     2041 tctaccggca gagagtgacc cggaactgcg tgaacgtgtt ccaggacttc aacgccgtgt
     2101 ttggcaagtg ctacaacaag gatgacacca agctgagcat cacccgcgac aaagtgaaga
     2161 aagagctgag ccggaagaac cggaacttcg tggaatacct gatcgagatg ctggaaaaca
     2221 ccctgaacag catgaacgac gatttcatca acaaggacaa tttcgacctg aacaactacg
     2281 tgaaagagtt cgagctgatc aattacctgc tgatccacga ggacagcgac atcttcctgg
     2341 aaacctacaa cctgatcagc ggcctcaaca gcaatatcga ggaaacctcc atcgagaagc
     2401 tgaagtacgc catcctgcag ggcaagcaga tcaactacaa gatcaaggac gacatctact
     2461 atatcctcaa gaacgcctac gccaagtact tcaagatcga cgtgtacaag aagggcaagc
     2521 tgctgtaccc caccctgtac taccaccgga acgccttcat caagagcttc gtggtggaat
     2581 tctttaacaa caacaaagtg tgcgagaaca ccaagtgccc cctgaatagc aactgctacg
     2641 tgatcgacga cgaagagaca tgccggtgcc tgcccggctt caacaacatt aagatcgatg
     2701 acgagatgaa ctgcgtcagg gacgacaccc tggactgctc cagaaacaac ggcggctgcg
     2761 acatccacgc caagtgcagc ttcattaaca agcagattgt gtgcgagtgc aaagacaagt
     2821 tcgagggcga cggcatctac tgcagctact ctggcgcgcc gtcgacctcc atcacggcct
     2881 ataagagtga gggggagtca gcggagttct ccttcccact caaccttgga gaggaaagcc
     2941 tgcagggaga gttgagatgg aaggcagaga aggctccttc ttcccagtcc tggatcacct
     3001 tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac tagcaacccc aagttccagc
     3061 tgtccgaaac gctcccactc acccttcaga taccccaggt ctcccttcag tttgctggtt
     3121 ctggcaacct gaccctgact ctggacagag ggatactgta tcaggaagtg aacctggtgg
     3181 tgatgaaagt gactcagccc gacagcaaca ctttgacctg tgaggtgatg ggacccacct
     3241 cacccaagat gagactgatc ttgaagcagg agaatcagga ggccagggtc tccaggcagg
     3301 agaaagtgat tcaagtgcag gcccctgaag caggggtgtg gcaatgtcta ctgagtgaag
     3361 gtgaagaggt caagatggac tccaagatcc aggttttatc caaagggttg aattccggca
     3421 gcctgcacca catcctggac gcccagaaga tgctgtggaa ccacagggac aggaacctgc
     3481 ctcctctggc ccctctgggc ccccaccacc atcaccacca ttgagtctag acacgtgatt
     3541 aattaaggat cccccgacct cgacctctgg ctaataaagg aaatttattt tcattgcaat
     3601 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     3661 ggtcgagatc cctcggagat ctctagctag aggatcgatc cccgccccgg acgaactaaa
     3721 cctgactacg acatctctgc cccttcttcg cggggcagtg catgtaatcc cttcagttgg
     3781 ttggtacaac ttgccaactg ggccctgttc cacatgtgac acgggggggg accaaacaca
     3841 aaggggttct ctgactgtag ttgacatcct tataaatgga tgtgcacatt tgccaacact
     3901 gagtggcttt catcctggag cagactttgc agtctgtgga ctgcaacaca acattgcctt
     3961 tatgtgtaac tcttggctga agctcttaca ccaatgctgg gggacatgta cctcccaggg
     4021 gcccaggaag actacgggag gctacaccaa cgtcaatcag aggggcctgt gtagctaccg
     4081 ataagcggac cctcaagagg gcattagcaa tagtgtttat aaggccccct tgttaaccct
     4141 aaacgggtag catatgcttc ccgggtagta gtatatacta tccagactaa ccctaattca
     4201 atagcatatg ttacccaacg ggaagcatat gctatcgaat tagggttagt aaaagggtcc
     4261 taaggaacag cgatatctcc caccccatga gctgtcacgg ttttatttac atggggtcag
     4321 gattccacga gggtagtgaa ccattttagt cacaagggca gtggctgaag atcaaggagc
     4381 gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt tagctaatag
     4441 aataactgct gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag
     4501 gtgacgcccc cagaataaaa tttggacggg gggttcagtg gtggcattgt gctatgacac
     4561 caatataacc ctcacaaacc ccttgggcaa taaatactag tgtaggaatg aaacattctg
     4621 aatatcttta acaatagaaa tccatggggt ggggacaagc cgtaaagact ggatgtccat
     4681 ctcacacgaa tttatggcta tgggcaacac ataatcctag tgcaatatga tactggggtt
     4741 attaagatgt gtcccaggca gggaccaaga caggtgaacc atgttgttac actctatttg
     4801 taacaagggg aaagagagtg gacgccgaca gcagcggact ccactggttg tctctaacac
     4861 ccccgaaaat taaacggggc tccacgccaa tggggcccat aaacaaagac aagtggccac
     4921 tctttttttt gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc gccctgcggt
     4981 tttggactgt aaaataaggg tgtaataact tggctgattg taaccccgct aaccactgcg
     5041 gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg ggaatacctg cataagtagg
     5101 tgggcgggcc aagatagggg cgcgattgct gcgatctgga ggacaaatta cacacacttg
     5161 cgcctgagcg ccaagcacag ggttgttggt cctcatattc acgaggtcgc tgagagcacg
     5221 gtgggctaat gttgccatgg gtagcatata ctacccaaat atctggatag catatgctat
     5281 cctaatctat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     5341 cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag cataggctat
     5401 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     5461 cctaatctgt atccgggtag catatgctat cctaatagag attagggtag tatatgctat
     5521 cctaatttat atctgggtag catatactac ccaaatatct ggatagcata tgctatccta
     5581 atctatatct gggtagcata tgctatccta atctatatct gggtagcata ggctatccta
     5641 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
     5701 atttatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
     5761 atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata tgctatcctc
     5821 atgataagct gtcaaacatg agaattttct tgaagacgaa agggcctcgt gatacgccta
     5881 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     5941 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     6001 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     6061 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     6121 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     6181 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     6241 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     6301 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     6361 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     6421 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     6481 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     6541 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
     6601 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     6661 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     6721 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     6781 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     6841 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     6901 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     6961 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     7021 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     7081 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     7141 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7201 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     7261 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7321 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     7381 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7441 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     7501 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     7561 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     7621 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     7681 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     7741 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     7801 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     7861 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     7921 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     7981 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     8041 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctctagcta
     8101 gaggtcgacc aattctcatg tttgacagct tatcatcgca gatccgggca acgttgttgc
     8161 cattgctgca ggcgcagaac tggtaggtat ggaagatcta tacattgaat caatattggc
     8221 aattagccat attagtcatt ggttatatag cataaatcaa tattggctat tggccattgc
     8281 atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc
//
