LOCUS       sequence-76209-e        7506 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full length PF13_0125.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7506)
  AUTHORS   Zenonos ZA, Rayner JC, Wright GJ
  TITLE     Towards a comprehensive Plasmodium falciparum merozoite cell surface
            and secreted recombinant protein library.
  JOURNAL   Malar J. 2014 Mar 12;13(1):93. doi: 10.1186/1475-2875-13-93.
  PUBMED    24620899
REFERENCE   2  (bases 1 to 7506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2014-03-12"; volume: "13(1):93. doi"; pages: " 
            10.1186/1475-2875-13-93"
COMMENT     Sequence Label: PF13_0125-bio-His
FEATURES             Location/Qualifiers
     source          1..7506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2670..2687
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2739..2794
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2793..2812)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5030..5048)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5116..5220
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5221..5289,5290..6081)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5439..5458)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6252..6840
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6741..6760
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6994..7011
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7128..7149
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7164..7169,7170..7187,7188..7194)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7202..7218
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7207..7229
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7226..7242
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7226..7242
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcaa
     1201 acaagatcac caagcagtac ggcttcaccc cctacagctt caactgcaac gccgagatca
     1261 tccggcggag cagctacggc tacaaccagg aaatcctgaa cgacgccgtg aacctgatcc
     1321 tggaaaacac ccacaagaac aagatcggca tcatccacaa gagcatcaga gagctgctga
     1381 gcggccggta caacagcctg aacccccaga aggtgctgct gcggatcagc aacatcatca
     1441 agcacggcca catcaactcc ctgtgcgaca acaaccaggc ctacgtgctg atcgacatcc
     1501 cccacatcat cttcaacgcc aacctgatcg gcgaccccaa gctgcagaga atcaactacc
     1561 tgatcaacaa tatcctgaac aacctggacg tgtgcagcag caagcggaag tacaacatcc
     1621 agctgaagtc caacgtggcc aacggctgcc tgagccagag cgacaaggat atcatcttcg
     1681 acaccaccaa gcccagcaac agcagaagca agggcctgca gctgagcccc tccaacaagt
     1741 tcttcagcat tctgctgaag tgctccgtgg acaacctggg cagcggcgac agcaccatgg
     1801 aatgcgtgca gaaagagctg gccaaggaca acaagaagat gagcgatacc tgcagccagt
     1861 gcttcaagga cagcgtgtcc tgcggcaaga gcaactgctg gctgccttgc ctgttcggca
     1921 gcccctgcag cgacagatgc tacaactgcg gcgtgaagta ctgcaacaaa gaactgatca
     1981 gatgcaccgg cctggaaaat ctgcccagcg cctgttctgg cgcgccgtcg acctccatca
     2041 cggcctataa gagtgagggg gagtcagcgg agttctcctt cccactcaac cttggagagg
     2101 aaagcctgca gggagagttg agatggaagg cagagaaggc tccttcttcc cagtcctgga
     2161 tcaccttctc cctaaagaac caaaaggtgt ctgtgcagaa gtctactagc aaccccaagt
     2221 tccagctgtc cgaaacgctc ccactcaccc ttcagatacc ccaggtctcc cttcagtttg
     2281 ctggttctgg caacctgacc ctgactctgg acagagggat actgtatcag gaagtgaacc
     2341 tggtggtgat gaaagtgact cagcccgaca gcaacacttt gacctgtgag gtgatgggac
     2401 ccacctcacc caagatgaga ctgatcttga agcaggagaa tcaggaggcc agggtctcca
     2461 ggcaggagaa agtgattcaa gtgcaggccc ctgaagcagg ggtgtggcaa tgtctactga
     2521 gtgaaggtga agaggtcaag atggactcca agatccaggt tttatccaaa gggttgaatt
     2581 ccggcagcct gcaccacatc ctggacgccc agaagatgct gtggaaccac agggacagga
     2641 acctgcctcc tctggcccct ctgggccccc accaccatca ccaccattga gtctagacac
     2701 gtgattaatt aaggatcccc cgacctcgac ctctggctaa taaaggaaat ttattttcat
     2761 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     2821 tcatttggtc gagatccctc ggagatctct agctagagga tcgatccccg ccccggacga
     2881 actaaacctg actacgacat ctctgcccct tcttcgcggg gcagtgcatg taatcccttc
     2941 agttggttgg tacaacttgc caactgggcc ctgttccaca tgtgacacgg ggggggacca
     3001 aacacaaagg ggttctctga ctgtagttga catccttata aatggatgtg cacatttgcc
     3061 aacactgagt ggctttcatc ctggagcaga ctttgcagtc tgtggactgc aacacaacat
     3121 tgcctttatg tgtaactctt ggctgaagct cttacaccaa tgctggggga catgtacctc
     3181 ccaggggccc aggaagacta cgggaggcta caccaacgtc aatcagaggg gcctgtgtag
     3241 ctaccgataa gcggaccctc aagagggcat tagcaatagt gtttataagg cccccttgtt
     3301 aaccctaaac gggtagcata tgcttcccgg gtagtagtat atactatcca gactaaccct
     3361 aattcaatag catatgttac ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa
     3421 gggtcctaag gaacagcgat atctcccacc ccatgagctg tcacggtttt atttacatgg
     3481 ggtcaggatt ccacgagggt agtgaaccat tttagtcaca agggcagtgg ctgaagatca
     3541 aggagcgggc agtgaactct cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc
     3601 taatagaata actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg
     3661 tttcaggtga cgcccccaga ataaaatttg gacggggggt tcagtggtgg cattgtgcta
     3721 tgacaccaat ataaccctca caaacccctt gggcaataaa tactagtgta ggaatgaaac
     3781 attctgaata tctttaacaa tagaaatcca tggggtgggg acaagccgta aagactggat
     3841 gtccatctca cacgaattta tggctatggg caacacataa tcctagtgca atatgatact
     3901 ggggttatta agatgtgtcc caggcaggga ccaagacagg tgaaccatgt tgttacactc
     3961 tatttgtaac aaggggaaag agagtggacg ccgacagcag cggactccac tggttgtctc
     4021 taacaccccc gaaaattaaa cggggctcca cgccaatggg gcccataaac aaagacaagt
     4081 ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca cacgccgccc
     4141 tgcggttttg gactgtaaaa taagggtgta ataacttggc tgattgtaac cccgctaacc
     4201 actgcggtca aaccacttgc ccacaaaacc actaatggca ccccggggaa tacctgcata
     4261 agtaggtggg cgggccaaga taggggcgcg attgctgcga tctggaggac aaattacaca
     4321 cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc atattcacga ggtcgctgag
     4381 agcacggtgg gctaatgttg ccatgggtag catatactac ccaaatatct ggatagcata
     4441 tgctatccta atctatatct gggtagcata ggctatccta atctatatct gggtagcata
     4501 tgctatccta atctatatct gggtagtata tgctatccta atttatatct gggtagcata
     4561 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     4621 tgctatccta atctgtatcc gggtagcata tgctatccta atagagatta gggtagtata
     4681 tgctatccta atttatatct gggtagcata tactacccaa atatctggat agcatatgct
     4741 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agcataggct
     4801 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     4861 atcctaattt atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     4921 atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct
     4981 atcctcatga taagctgtca aacatgagaa ttttcttgaa gacgaaaggg cctcgtgata
     5041 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     5101 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     5161 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     5221 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     5281 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     5341 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     5401 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5461 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5521 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5581 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5641 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5701 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5761 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5821 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5881 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5941 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     6001 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     6061 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     6121 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     6181 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6241 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6301 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6361 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     6421 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6481 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6541 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6601 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6661 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6721 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6781 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6841 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6901 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6961 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     7021 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     7081 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     7141 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     7201 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc
     7261 tagctagagg tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt
     7321 tgttgccatt gctgcaggcg cagaactggt aggtatggaa gatctataca ttgaatcaat
     7381 attggcaatt agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc
     7441 cattgcatac gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat
     7501 gaccgc
//
