LOCUS       sequence-76212-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ampicillin resistant; entry clone encoding reverse TetR (tetR38) and
            contains attL4 and attL1 sites for recombination into a Gateway 
            destination vector at the attR4 and attR1 sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Kim JH, O'Brien KM, Sharma R, Boshoff HI, Rehren G, Chakraborty S, 
            Wallach JB, Monteleone M, Wilson DJ, Aldrich CC, Barry CE 3rd, Rhee 
            KY, Ehrt S, Schnappinger D
  TITLE     A genetic strategy to identify targets for the development of drugs 
            that prevent bacterial persistence.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Nov 4.
  PUBMED    24191058
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Nov 4."
COMMENT     Sequence Label: pEN41A-T38S38
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(43..166)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ctcgaattct acaggtcact aataccatct aagtagttgg ttcatagtga ctgcatatgt
       61 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
      121 tttatatcat tttacgtttc tcgttcaact tttttgtaca aacttgaata ttggatcacg
      181 ccgcgagcgt gtcggtgttc gaagacctgg gaatccagca ggcgcccaac agcaccgtct
      241 gctccgtctt ctccagaaag gcgactacgc ggtcctcctc gtcggtgggt acgcagccga
      301 atgcccgcgg gtcgcccacc cgcaccagtt gcagcgtgac atcggctcga ttgcttcggt
      361 ggaagtccag cagttgggcc agatccgcga gggccccccg tgaacatcgt cgtggtaacc
      421 agcgtgtcgg ctttggctgt ggccgtggtg cattcggtgg cgtttgcgat caaggggcgt
      481 cacggcgagg acctggtcga ccgctggaac cgtgagggca tcggtgcaca ccgcgggatc
      541 agaccgtcga cgaaccattt gaatccgacc ggtacctcaa cgagttgacg gccgatcacg
      601 tccaggttat gttcgatgac gatcaccgta ttgcccttgt cgaccaggcc gttgatctga
      661 cctagggtac catcaggagg aatcacttcg caatgtcccg cctggacaag tccaaggtca
      721 tcaactccgc cctggccctg ggcaacgagg tcggcatcga gggcgtgacc acccgcaagc
      781 tggcccagaa gctgggcgtc gcgcagaaga ccctgtactg gcacgtcaag aacaagcggg
      841 ccctgctgga cgccctggcg gtggagatcc tggcgcgcca ccacgactac tcgctgccgg
      901 cggccggcga gtcgtggcag tcgttcctgc gcaacaacg
//
