LOCUS       sequence-76213-e         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Galactose-inducible expression of cytosolic S. pyogenes SrtA in 
            yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Strijbis K, Spooner E, Ploegh HL
  TITLE     Protein ligation in living cells using sortase.
  JOURNAL   Traffic. 2012 Jun;13(6):780-9. doi: 
            10.1111/j.1600-0854.2012.01345.x. Epub 2012 Mar 23.
  PUBMED    22348280
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1600-0854.2012.01345.x"; journalName: "Traffic"; date: 
            "2012-06"; volume: "13"; issue: "6"; pages: "780-9"
COMMENT     Sequence Label: pRS303-pGAL-HA-SrtAstrep (pKS78)
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        10..28
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(568..585)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(595..621)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(666..689)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 gccagctcga attaaccctc actaaaggga acaaaagctg gtaccgggcc ccccctcgag
       61 ctaggtagat acttggttat aagaatgatt aaaagctttc aatacatcgg cgggcgcttt
      121 atcaaagtcg tattctgttt ttagttctcc tttgacaata atacgttctg ttgcttcgat
      181 atctgtacaa gtcactaaag tcacttcttt gagaccagct gtatcatcga taacatcaac
      241 gcgttcagga gctaccgtga aaacatcttt tatgatgtat tcgtaaattt tttctttatc
      301 tgttaaatag atggacatcc cattttgtgc tctttcaagc ggcgaaaaga gcatttgaga
      361 tgaacctgta attccaaaaa tatgatgact ggcaagagaa taattatttt ctcctcccat
      421 aacttgttct tctttcatcg ttcctgcgcc ataaataagc tcagtatttc ctaaaccttt
      481 aaaaattggt aaattaatgc caagctctgg tatggcaatg ccccctataa caggaagttg
      541 ctgagccgcc atttgtgctt gcaagacgga tccgcgcggc accaggccgc tgcttgcgta
      601 gtctggtacg tcgtatgggt agctgctgcg catggtggct ctagaatccg gggttttttc
      661 tccttgacgt taaagtatag aggtatatta acaatttttt gttgatactt ttattacatt
      721 ttgaaat
//
