LOCUS       sequence-76214-e         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Galactose-inducible expression of ER-luminal S. pyogenes SrtA in 
            yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Strijbis K, Spooner E, Ploegh HL
  TITLE     Protein ligation in living cells using sortase.
  JOURNAL   Traffic. 2012 Jun;13(6):780-9. doi: 
            10.1111/j.1600-0854.2012.01345.x. Epub 2012 Mar 23.
  PUBMED    22348280
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1600-0854.2012.01345.x"; journalName: "Traffic"; date: 
            "2012-06"; volume: "13"; issue: "6"; pages: "780-9"
COMMENT     Sequence Label: pRS303-pGAL-SPPHO5-G-SrtAstrep-HA-HDEL (pKS82)
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        11..29
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(95..121)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(742..765)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 cgccagctcg aattaaccct cactaaaggg aacaaaagct ggtaccgggc cccccctcga
       61 gctacagctc gtcatgctcg cttgcggccc catttgcgta gtctggtacg tcgtatgggt
      121 atgcggccgc gtcgacggta gatacttggt tataagaatg attaaaagct ttcaatacat
      181 cggcgggcgc tttatcaaag tcgtattctg tttttagttc tcctttgaca ataatacgtt
      241 ctgttgcttc gatatctgta caagtcacta aagtcacttc tttgagacca gctgtatcat
      301 cgataacatc aacgcgttca ggagctaccg tgaaaacatc ttttatgatg tattcgtaaa
      361 ttttttcttt atctgttaaa tagatggaca tcccattttg tgctctttca agcggcgaaa
      421 agagcatttg agatgaacct gtaattccaa aaatatgatg actggcaaga gaataattat
      481 tttctcctcc cataacttgt tcttctttca tcgttcctgc gccataaata agctcagtat
      541 ttcctaaacc tttaaaaatt ggtaaattaa tgccaagctc tggtatggca atgcccccta
      601 taacaggaag ttgctgagcc gccatttgtg cttgcaagac actggatcct aagggaatgg
      661 tacctgcatt ggccaaagaa gcggctaaaa ttgaataaac aacagattta aacattctag
      721 aatccggggt tttttctcct tgacgttaaa gtatagaggt atattaacaa ttttttgttg
      781 atacttttat tacat
//
