LOCUS       sequence-76216-e         611 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Kanamycin resistant; expresses luxAB-DAS+4 under the control of a 
            promoter containing PtetO-4C5G; integrates into the 
            mycobacteriophage att-L5 site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Kim JH, O'Brien KM, Sharma R, Boshoff HI, Rehren G, Chakraborty S, 
            Wallach JB, Monteleone M, Wilson DJ, Aldrich CC, Barry CE 3rd, Rhee 
            KY, Ehrt S, Schnappinger D
  TITLE     A genetic strategy to identify targets for the development of drugs 
            that prevent bacterial persistence.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Nov 4.
  PUBMED    24191058
REFERENCE   2  (bases 1 to 611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Nov 4."
COMMENT     Sequence Label: pGMCK-0X750-luxAB-DAS+4
FEATURES             Location/Qualifiers
     source          1..611
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..32
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    36..60
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(232..256)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cgccaagcta tcaactttgt atagaaaagt tgaattcaag tttgtacaaa aaagcaggct
       61 gctaccaggc ctagatctgg ggaccctaga ggtccccttt tttattttaa aaattttttc
      121 acgtgccagc cgattggtcg ctctgaccag ggaaaatagc cctctgacct ggggatttgc
      181 gatccccgtc agtgacggag ataattgtga gcgctcacaa ccgcatgcac cacccagctt
      241 tcttgtacaa agtggttaat taacaggatc cccaaataag gaaatgttat gaaatttgga
      301 aacttccttc tcacttatca gccacctgag ctatctcaga ccgaagtgat gaagcgattg
      361 gttaatctgg gcaaagcgtc tgaaggttgt ggcttcgaca ccgtttggtt gctagagcac
      421 cacttcactg aatttgggtt gttagggaat ccttatgttg ctgccgcaca cctattaggt
      481 gcgacagaaa cgctcaacgt tggcactgca gctatcgtat tgccgactgc ccatccggtt
      541 cgacaagcag aagacgtaaa cctactggat caaatgtcaa aaggacgatt ccgttttggt
      601 atttgtcgcg g
//
