LOCUS       sequence-76217-e         624 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Kanamycin resistant; expresses luxAB-DAS+4 under the control of a 
            promoter containing PtetO-4C5G; constitutively expresses reverse 
            TetR (tetR38); integrates into the mycobacteriophage att-L5 site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Kim JH, O'Brien KM, Sharma R, Boshoff HI, Rehren G, Chakraborty S, 
            Wallach JB, Monteleone M, Wilson DJ, Aldrich CC, Barry CE 3rd, Rhee 
            KY, Ehrt S, Schnappinger D
  TITLE     A genetic strategy to identify targets for the development of drugs 
            that prevent bacterial persistence.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Nov 4.
  PUBMED    24191058
REFERENCE   2  (bases 1 to 624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Nov 4."
COMMENT     Sequence Label: pGMCK-T38S38-750-luxAB-DAS+4
FEATURES             Location/Qualifiers
     source          1..624
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..31
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gccaagctat caactttgta tagaaaagtt gcagctggct agcgagtcat gaggtcacca
       61 tggtcattag gagccgctct cgcacttcag ctgcttctcc aagccgcaga tgatcagctc
      121 caggccgaac aagaaggccg gctcggcgcc ctggtggtcg aacagctcga tggcctggcg
      181 cagcaacggc ggcatgctgt cggtggtcgg ggtctcgcgc tcctccttgg cggccgtgtg
      241 ctcctggtcc tccaacacgc agcccagggt gaagtggccc acggccgaca gggcgtacaa
      301 cgcgttctcc agcgagaagc cgttctcggt catgaagcgc agctgggtct ccacggtgtc
      361 gtactgcttc tcgtccgggc gggtgcccag gtgcaccttg gcgccgtcgc ggtagcgcag
      421 cagcgcgcgg cggaacgaca tggcgttgtt gcgcaggaac gactgccacg actcgccggc
      481 cgccggcagc gagtagtcgt ggtggcgcgc caggatctcc accgccaggg cgtccagcag
      541 ggcccgcttg ttcttgacgt gccagtacag ggtcttctgc gcgacgccca gcttctgggc
      601 cagcttgcgg gtggtcacgc cctc
//
