LOCUS       sequence-76218-e         689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nourseothricin resistant; expresses sspB under the control of a 
            promoter containing PtetO and constitutively expressses single-chain
            TetR10 (tsc10); integrates into mycobacteriophage att-Tweety site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Kim JH, O'Brien KM, Sharma R, Boshoff HI, Rehren G, Chakraborty S, 
            Wallach JB, Monteleone M, Wilson DJ, Aldrich CC, Barry CE 3rd, Rhee 
            KY, Ehrt S, Schnappinger D
  TITLE     A genetic strategy to identify targets for the development of drugs 
            that prevent bacterial persistence.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Nov 4.
  PUBMED    24191058
REFERENCE   2  (bases 1 to 689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Nov 4."
COMMENT     Sequence Label: pGMCtN-TSC10M1-sspB
FEATURES             Location/Qualifiers
     source          1..689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    27..47
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    576..600
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 acctctaggg tcccctgcag cttaagcaac tatgtataat aaagttgcta ttacttcacg
       61 acgcgcaggg ccgggcgacc gccgcgcggc ggctggggcg gctcatcgtc cgggtgggtg
      121 tcatcgtcgt ggtccggctt gtcgccgtcg atcaccgaca tcacggtctc gttgtcggcc
      181 gaggcctcct cgtcgttcat gatcgaggtg tcctcgtcgt aggccgcctc cggctcgaac
      241 atggtgccgg cgccgttctc gcgggcgtag atggccagca cggccgccag cggcaccgag
      301 acctgccgcg ggatgccacc gaaacgcgcg ttgaagcgca cctcgtcgtt cgccagctcc
      361 aggttgccga cggcgcgcgg cgcgatgttc agcacgatct ggccgtcgcg cgcgtactcc
      421 atcggcacct gcacgcccgg cagggtcacg tccaccacca ggtgcggggt cagctggttg
      481 tccagcaacc actcgtagaa ggcgcgcagc aggtagggcc ggcgcggggt cagctgcgac
      541 aggtccatat gtatatctcc ttcgcatgcc tcgagcccac tttgtacaag aaagctgggt
      601 gcatgcggat cgtgctcatt tcgggcggcg aatctctcgg cgtcgaaatg atgtatgccg
      661 tgctggtcgt acacacgggt actcccaga
//
