LOCUS       sequence-76219-e         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Promoterless luc2 donor plasmid for MPRA library construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Melnikov A, Zhang X, Rogov P, Wang L, Mikkelsen TS
  TITLE     Massively parallel reporter assays in cultured Mammalian cells.
  JOURNAL   J Vis Exp. 2014 Aug 17;(90). doi: 10.3791/51719.
  PUBMED    25177895
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Vis 
            Exp."; date: "2014-08-17"; volume: "(90). doi"; pages: " 
            10.3791/51719"
COMMENT     Sequence Label: pMPRAdonor1
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..46
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     38..55
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     39..55
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(71..87)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(71..87)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(84..106)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    95..111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(119..125,126..143,144..149))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 agtttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatc
       61 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      121 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactta
      181 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
      241 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tagggtggtt
      301 tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag
      361 agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg
      421 gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata
      481 tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga
      541 tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt
      601 tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg
      661 cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg
      721 cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc
      781 gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga
      841 aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc
      901 ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta
      961 caggcttcga c
//
