LOCUS       sequence-76221-e         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Hygromycin resistant; expresses luxAB-DAS+4 under the control of a 
            promoter containing PtetO-4C5G; replicates episomally.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Kim JH, O'Brien KM, Sharma R, Boshoff HI, Rehren G, Chakraborty S, 
            Wallach JB, Monteleone M, Wilson DJ, Aldrich CC, Barry CE 3rd, Rhee 
            KY, Ehrt S, Schnappinger D
  TITLE     A genetic strategy to identify targets for the development of drugs 
            that prevent bacterial persistence.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Nov 4.
  PUBMED    24191058
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Nov 4."
COMMENT     Sequence Label: pGMEH-0X750-luxAB-DAS+4
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..27
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    31..55
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(227..251)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agctatcaac tttgtataga aaagttgaat tcaagtttgt acaaaaaagc aggctgctac
       61 caggcctaga tctggggacc ctagaggtcc ccttttttat tttaaaaatt ttttcacgtg
      121 ccagccgatt ggtcgctctg accagggaaa atagccctct gacctgggga tttgcgatcc
      181 ccgtcagtga cggagataat tgtgagcgct cacaaccgca tgcaccaccc agctttcttg
      241 tacaaagtgg ttaattaaca ggatccccaa ataaggaaat gttatgaaat ttggaaactt
      301 ccttctcact tatcagccac ctgagctatc tcagaccgaa gtgatgaagc gattggttaa
      361 tctgggcaaa gcgtctgaag gttgtggctt cgacaccgtt tggttgctag agcaccactt
      421 cactgaattt gggttgttag ggaatcctta tgttgctgcc gcacacctat taggtgcgac
      481 agaaacgctc aacgttggca ctgcagctat cgtattgccg actgcccatc cggttcgaca
      541 agcagaagac gtaaacctac tggatcaaat gtcaaaagga cgattccgtt ttggtatttg
      601 tcgcggtttg tacgataaag attttcgtgt ctttggtaca gacatggata acagccgagc
      661 cttaatggac tgttggtatg acttgatgaa agaaggcttc aatgaaggct atatcgcggc
      721 ggataacgaa catattaagt tcccgaaaat ccaactgaat ccatcggctt a
//
