LOCUS       sequence-76223-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Hygromycin resistant; expresses luxAB-DAS+4 under the control of a 
            promoter containing PtetO-4C5G; constitutively expresses reverse 
            TetR (tetR38); replicates episomally.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Kim JH, O'Brien KM, Sharma R, Boshoff HI, Rehren G, Chakraborty S, 
            Wallach JB, Monteleone M, Wilson DJ, Aldrich CC, Barry CE 3rd, Rhee 
            KY, Ehrt S, Schnappinger D
  TITLE     A genetic strategy to identify targets for the development of drugs 
            that prevent bacterial persistence.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Nov 4.
  PUBMED    24191058
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Nov 4."
COMMENT     Sequence Label: pGMEH-T38M-750-luxAB-DAS+4
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..27
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    850..874
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agctatcaac tttgtataga aaagttgcag ctggctagcg agtcatgagg tcaccatggt
       61 cattaggagc cgctctcgca cttcagctgc ttctccaagc cgcagatgat cagctccagg
      121 ccgaacaaga aggccggctc ggcgccctgg tggtcgaaca gctcgatggc ctggcgcagc
      181 aacggcggca tgctgtcggt ggtcggggtc tcgcgctcct ccttggcggc cgtgtgctcc
      241 tggtcctcca acacgcagcc cagggtgaag tggcccacgg ccgacagggc gtacaacgcg
      301 ttctccagcg agaagccgtt ctcggtcatg aagcgcagct gggtctccac ggtgtcgtac
      361 tgcttctcgt ccgggcgggt gcccaggtgc accttggcgc cgtcgcggta gcgcagcagc
      421 gcgcggcgga acgacatggc gttgttgcgc aggaacgact gccacgactc gccggccgcc
      481 ggcagcgagt agtcgtggtg gcgcgccagg atctccaccg ccagggcgtc cagcagggcc
      541 cgcttgttct tgacgtgcca gtacagggtc ttctgcgcga cgcccagctt ctgggccagc
      601 ttgcgggtgg tcacgccctc gatgccgacc tcgttgccca gggccagggc ggagttgatg
      661 accttggact tgtccaggcg ggacattgcg aagtgattcc tcctgatggt accggatccg
      721 tggcaggagg gtcgcctgcg tcggcaactc gcccaggcta cacgagagcc ctcgactgcg
      781 aaaatgccga ccaggcacca ccgcactcca ggcttaaccc ggtgcgacga tccaatatta
      841 cgagatctga caagtttgta caaaaaagca ggctaccagg cctagatctg gggaccctag
      901 aggtcccctt ttttatttta aaaatttttt cacgtgccag ccgattgg
//
