LOCUS       sequence-76228-e         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express YOD1 with a N-terminal Flag-tag in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Ernst R, Mueller B, Ploegh HL, Schlieker C
  TITLE     The otubain YOD1 is a deubiquitinating enzyme that associates with 
            p97 to facilitate protein dislocation from the ER.
  JOURNAL   Mol Cell. 2009 Oct 9;36(1):28-38. doi: 10.1016/j.molcel.2009.09.016.
  PUBMED    19818707
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2009.09"; journalName: "Mol Cell"; date: 
            "2009-10-9- 9"; volume: "36"; issue: "1"; pages: "28-38"
COMMENT     Sequence Label: pcDNA3-Flag-YOD1
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             20..43
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 acttaagctt gccaccatgg attacaagga tgacgacgat aagatgtttg gccccgctaa
       61 aggtcgccat tttggagtcc acccggcgcc tggtttcccc ggcggcgtct cccaacaggc
      121 tgccgggacc aaagctggcc ccgcgggtgc ctggcctgtg ggcagccgga ccgacacgat
      181 gtggcggctc cgctgcaagg ccaaggacgg cacccatgtt ttgcaggggc tgtccagccg
      241 gacccgggtg cgggaactcc agggccaaat tgccgccatc accgggatcg cccccggcgg
      301 tcagcgaatc ctcgtcggat accctcccga gtgcctggat ctcagcaatg gggataccat
      361 tctggaagac ttgcccatcc aatctggtga catgctgatc attgaagaag accaaaccag
      421 gcccagaagt tcacctgcat ttactaaacg tggtgcttct agttacgtca gggaaacttt
      481 gcctgtgctt accagaaccg tggtcccagc agacaactct tgcctcttta ctagtgtgta
      541 ctatgtcgtc gaaggaggag tcttgaatcc agcttgtgcc cctgagatga gacgcctcat
      601 agcacaaatt gtagcaagcg atccagactt ctatagtgag gcaatactgg gaaaaacaaa
      661 tcaagagtac tgtgactgga tcaaaaggga tgacacttgg ggaggagcaa tagagatatc
      721 gattttgtcc aagttttacc aatgtgaaat atgtgtagtg gatacacaga cagtaagaat
      781 tgatcgtttt ggggaagatg caggatatac caaaagggtt ctgcttattt atgatggcat
      841 ccactatgat ccacttcagc gtaacttccc tgatccagat acacctcctc tgaccatttt
      901 ctcctctaat gatgatattg ttcttgtaca agcactggaa ttagca
//
