LOCUS       sequence-76229-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of gRNA under control of the Drosophila U6:1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Port F, Chen HM, Lee T, Bullock SL
  TITLE     Optimized CRISPR/Cas tools for efficient germline and somatic genome
            engineering in Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500.
  PUBMED    25002478
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2014 Jul 7. pii: 201405500."
COMMENT     Sequence Label: pCFD1-dU6:1gRNA
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(285..360)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tgttggttgg cacaccacaa atatactgtt gccgagcaca attgtctaga atgcatacgc
       61 attaagcgaa cattaaaaag atgtgaaaac ataactatta tgtctaaata aacacacgtc
      121 agatgtatgt acgtcaacgg aaaaccattg tctatatatt acaattacta aatacatacc
      181 aaattgaata catattgatg aaaaataata aatactggcg aaagcaaaaa aacgaaacat
      241 ttttatttta ttgaacaact ctcaggctcc aggtaggcaa aaaagcaccg actcggtgcc
      301 actttttcaa gttgataacg gactagcctt attttaactt gctatttcta gctctaaaac
      361 aggtcttctc gaagaccccg aagttcaccc ggatatcttt cctatatata ctgtactctt
      421 gcagaatatg tacttgggaa tcataaggat gcgaaggacg tacgcgctgt gtctgtaact
      481 aaggggttgc tttcgggtac ctaccatgcc aatgatggga ctaccgccgg ataggaatcc
      541 cttttgggtg cacaccgtca cctccagttc cgtctgcctg agttcggcaa tggaaatcag
      601 gtactcaaag gatgcatcgt agacggggtt gcagttgtcc ttgatcacgc tcgtcttgcg
      661 tttcgactcc ttggtgcgtc caggcaacag atacagctta acatacggat cggggatatt
      721 gctgggatcg cgaagtggta tcttctggat tttgtgtatg gtcacgtcta gtttttgacg
      781 ctgggcgctg tagcggatcg acaactgcat tcgtccaagg ccatgttcgc cgggtga
//
