LOCUS       sequence-76233-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of gRNA under control of the Drosophila U6:3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Port F, Chen HM, Lee T, Bullock SL
  TITLE     Optimized CRISPR/Cas tools for efficient germline and somatic genome
            engineering in Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500.
  PUBMED    25002478
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2014 Jul 7. pii: 201405500."
COMMENT     Sequence Label: pCFD3-dU6:3gRNA
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(282..357)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(376..796)
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
ORIGIN
        1 tggttggcac accacaaata tactgttgcc gagcacaatt gtctagaatg catacgcatt
       61 aagcgaacat taaaaagatg tgaaaacata actattatgt ctaaataaac acacgtcaga
      121 tgtatgtacg tcaacggaaa accattgtct atatattaca attactaaat acataccaaa
      181 ttgaatacat attgatgaaa aataataaat actggcgaaa gcaaaaaaac gaaacatttt
      241 tattttattg aacaactctc aggctccagg taggcaaaaa agcaccgact cggtgccact
      301 ttttcaagtt gataacggac tagccttatt ttaacttgct atttctagct ctaaaacagg
      361 tcttctcgaa gaccccgacg ttaaattgaa aataggtcta tatatacgaa ctgagtctgg
      421 aaaaagaagt tgagaattat aaaaagtagt gagcactggc gccctctctg cttggcgagc
      481 taaccttttc gcctcttggc tgagtaggtg gcgtttcatt ctactctgta aaattaatgt
      541 agaattgaaa cactatggtc aaaaaatact taggggcata agttataaaa cgtatgaaat
      601 gaatttttat caacctgggc tattcaaaaa ttttcgaatt attttatgta ttttttttaa
      661 tcgtttttca ttataggtta aaatacactt taaaaggaat tctttcctgt aaaataaata
      721 taaataaata tgctttattg acagaaaatt tgatgttttt gtatttgagt aggagcaatc
      781 acaggtgagc aaaaaagaat tcagatctca attgatcggc taaatggtat ggcaagaaaa
      841 ggtatgcaat ataataatct tttattgggt atgcaacgaa aatttgtttc gtcaa
//
