LOCUS       sequence-76235-e         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Kanamycin and Chloramphenicol resistant; destination vector 
            engineered to integrate into the mycobacteriophage att-L5 site and 
            designed to be used with the multisite Gateway cloning system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Kim JH, O'Brien KM, Sharma R, Boshoff HI, Rehren G, Chakraborty S, 
            Wallach JB, Monteleone M, Wilson DJ, Aldrich CC, Barry CE 3rd, Rhee 
            KY, Ehrt S, Schnappinger D
  TITLE     A genetic strategy to identify targets for the development of drugs 
            that prevent bacterial persistence.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Nov 4.
  PUBMED    24191058
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Nov 4."
COMMENT     Sequence Label: pDE43-MCK
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..130
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(171..476)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(273..292)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 agctatcaac tttgtataga aaagttgaac gagaaacgta aaatgatata aatatcaata
       61 tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc
      121 agtcactatg gtcgacctgc agactggctg tgtataaggg agcctgacat ttatattccc
      181 cagaacatca ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact
      241 tcttccccga taacggagac cggcacactg gccatatcgg tggtcatcat gcgccagctt
      301 tcatccccga tatgcaccac cgggtaaagt tcacggggga ctttatctga cagcagacgt
      361 gcactggcca gggggatcac catccgtcgc ccgggcgtgt caataatatc actctgtaca
      421 tccacaaaca gacgataacg gctctctctt ttataggtgt aaaccttaaa ctgcatttca
      481 ccagcccctg ttctcgtcgg caaaagagcc gttcatttca ataaaccggg cgacctcagc
      541 catcccttcc tgattttccg ctttccagcg ttcggcacgc agacgacggg cttcattctg
      601 catggttgtg cttaccgaac cggagatatt gacatcatat atgccttgag caactgatag
      661 ctgtcgctgt caactgtcac tgtaatacgc tgcttcatag catccctctt tttgacatac
      721 ttcgggtata catatcagta tatattctta taccgcaaaa atcagcgcgc aaatacgcat
      781 actgttatct ggcttttagt aagccggatc ctctagatta cgccccgcct gccactcatc
      841 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
      901 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
      961 gg
//
