LOCUS       sequence-76238-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nourseothricin resistant; expresses sspB under the control of a 
            promoter containing PtetO and constitutively expressses single-chain
            TetR10 (tsc10); integrates into mycobacteriophage att-Tweety site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Kim JH, O'Brien KM, Sharma R, Boshoff HI, Rehren G, Chakraborty S, 
            Wallach JB, Monteleone M, Wilson DJ, Aldrich CC, Barry CE 3rd, Rhee 
            KY, Ehrt S, Schnappinger D
  TITLE     A genetic strategy to identify targets for the development of drugs 
            that prevent bacterial persistence.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Nov 4.
  PUBMED    24191058
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Nov 4."
COMMENT     Sequence Label: pGMCtN-TSC10M1-sspB
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    15..35
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ttacgccaag ctatcaactt tgtatagaaa agttgcagct ggctagcgag tcatgaggtc
       61 accatggtca ttaggagccg ctctcgcact tcagctgctt ctccaagccg cagatgatca
      121 gctccaggcc gaacaagaag gccggctcgg cgccctggtg gtcgaacagc tcgatggcct
      181 ggcgcagcaa cggcggcatg ctgtcggtgg tcggggtctc gcgctcctcc ttggcgacct
      241 ggtgctcctg gtcctccaac acgcagccca gggtgaagtg gcccacggcc gacagggcgt
      301 acaacgcgtt ctccagcgag aagccctgct ggcacaggaa ggccaactgg ttctccaggg
      361 tctcgtactg cttctcggtc ggccgggtgc ccaggtgcac cttggcgccg tcgcggtggc
      421 tcagcaaggc gcagcggaag ctcttggcgt tgttgcgcag gaagtcctgc cagctctcgc
      481 cctccagcgg gcagaagtgg gtgtggtggc ggtccaacat ctcgatggcc agggcgtcga
      541 gcagggcccg cttgttcttg acgtgccagt acagggtcgg ctgctcgacg cccagcttct
      601 gggccagctt gcgggtggtc aggccctcga tgccgacctc gttcagcagc tccagggcgg
      661 agttgatgac cttggacttg tccaggcggg atctagagcc acctccgccc gatccgccac
      721 ctccagagcc tccaccgcca gaaccgcctc caccggatcc accgcctcct gagtcgcccg
      781 actcacactt caactgcttc tccagcccac aaatgatgag ttccaaaccg aacaggaacg
      841 cgggctccgc accctgatga tcgaagagct cgatcgcctg ccggagcagc gggggcatcg
      901 aatccgtggt gggcgtctcc cgctcttcct tc
//
