LOCUS       sequence-76243-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses VSVG fused to UVR8 for light-triggered trafficking.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Chen D, Gibson ES, Kennedy MJ
  TITLE     A light-triggered protein secretion system.
  JOURNAL   J Cell Biol. 2013 May 13;201(4):631-40. doi: 10.1083/jcb.201210119.
  PUBMED    23671313
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2013-05-13- 13"; volume: "201"; 
            issue: "4"; pages: "631-40"
COMMENT     Sequence Label: VSVG-mEOS2-2xUVR8
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..26
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           95..134
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
ORIGIN
        1 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
       61 atagaagaca ccgggaccga tccagcctcc gggggatctt ggtggcgtga aactcccgca
      121 cctcttcggc cagcgaattc aagcttcgaa ttcgccatga agtgcctttt gtacttagct
      181 tttttattca tcggggtgaa ttgcaagttc accatagttt ttccacacaa ccaaaaagga
      241 aactggaaaa atgttccttc caattaccat tattgcccgt caagctcaga tttaaattgg
      301 cataatgact taataggcac agccttacaa gtcaaaatgc ccaagagtca caaggctatt
      361 caagcagacg gttggatgtg tcatgcttcc aaatgggtca ctacttgtga tttccgctgg
      421 tacggaccga agtatataac acattccatc cgatccttca ctccatctgt agaacaatgc
      481 aaggaaagca ttgaacaaac gaaacaagga acttggctga atccaggctt ccctcctcaa
      541 agttgtggat atgcaactgt gacggatgct gaagcagcga ttgtccaggt gactcctcac
      601 catgtgcttg ttgatgaata cacaggagaa tgggttgatt cacagttcat caacggaaaa
      661 tgcagcaatg acatatgccc cactgtccat aactccacaa cctggcattc cgactataag
      721 gtcaaagggc tatgtgattc taacctcatt tccatggaca tcacctcctt ctcagaggac
      781 ggagagctat catccctagg aaaggagggc acagggttca gaagtaacta ctttgcttat
      841 gaaaccggag acaaggcctg caaaatgcag tactgcaagc attgggga
//
