LOCUS       sequence-76244-e         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses Arf1T31N in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Moorhead AM, Jung JY, Smirnov A, Kaufer S, Scidmore MA
  TITLE     Multiple host proteins that function in 
            phosphatidylinositol-4-phosphate metabolism are recruited to the 
            chlamydial inclusion.
  JOURNAL   Infect Immun. 2010 May;78(5):1990-2007. doi: 10.1128/IAI.01340-09. 
            Epub 2010 Mar 15.
  PUBMED    20231409
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/IAI.01340-09"; journalName: "Infect Immun"; date: 
            "2010-05"; volume: "78"; issue: "5"; pages: "1990-2007"
COMMENT     Sequence Label: Arf1T31N-EGFP
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      656..665
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(707..728)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(968..987)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat
       61 ctcgagctca agcttcgaat tcatggggaa catcttcgcc aacctcttca agggcctttt
      121 tggcaaaaaa gaaatgcgca tcctcatggt gggcctggat gctgcaggga agaacacgat
      181 cctctacaag cttaagctgg gtgagatcgt gaccaccatt cccaccatag gcttcaacgt
      241 ggaaaccgtg gagtacaaga acatcagctt cactgtgtgg gacgtgggtg gccaggacaa
      301 gatccggccc ctgtggcgcc actacttcca gaacacacaa ggcctgatct tcgtggtgga
      361 cagcaatgac agagagcgtg tgaacgaggc ccgtgaggag ctcatgagga tgctggccga
      421 ggacgagctc cgggatgctg tcctcctggt gttcgccaac aagcaggacc tccccaacgc
      481 catgaatgcg gccgagatca cagacaagct ggggctgcac tcactacgcc acaggaactg
      541 gtacattcag gccacctgcg ccaccagcgg cgacgggctc tatgaaggac tggactggct
      601 gtccaatcag ctccggaacc agaaggtcga cggtaccgcg ggcccgggat ccatcgccac
      661 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      721 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      781 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      841 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
      901 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      961 cttcaaggac gacggcaact acaagacccg cgccgag
//
