LOCUS       sequence-76247-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of mCherry-Vav1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Strijbis K, Tafesse FG, Fairn GD, Witte MD, Dougan SK, Watson N, 
            Spooner E, Esteban A, Vyas VK, Fink GR, Grinstein S, Ploegh HL
  TITLE     Bruton's Tyrosine Kinase (BTK) and Vav1 contribute to 
            Dectin1-dependent phagocytosis of Candida albicans in macrophages.
  JOURNAL   PLoS Pathog. 2013 Jun;9(6):e1003446. doi: 
            10.1371/journal.ppat.1003446. Epub 2013 Jun 27.
  PUBMED    23825946
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2013-06"; volume: "9(6):e1003446. doi"; pages: " 
            10.1371/journal.ppat.1003446. Epub 2013 Jun 27"
COMMENT     Sequence Label: pMSCV-mCherry-Vav1
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             43..750
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(188..206)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     456..475
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     650..673
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gccctcactc cttctctagg cgccggaatt agatctgcca ccatggtgag caagggcgag
       61 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
      121 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
      181 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
      241 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
      301 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
      361 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
      421 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
      481 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
      541 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ccgaggtcaa gaccacctac
      601 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gctggacatc
      661 acctcccaca acgaggacta caccatcgtg gaacagtacg agcgcgccga gggccgccac
      721 tccaccggcg gcatggacga gctgtacaag ggaggaggag gatcaggagg aggaggatca
      781 cctagggagc tctggcgaca gtgcacca
//
