LOCUS       sequence-76257-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  TITLE     Johnson lab plasmids transferred from original pMEX to pcDNA
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA 3.1(-) mouse C/EBP beta (LIP)
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      155..164
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(645..662)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    651..762
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gtttaaacgg gccctctaga ctcgagcggc
      121 cgccactgtg ctggatatct gcagaattcc cgcaccatgg cggccggttt cccgttcgcc
      181 ctgcgcgcct acctgggcta ccaggcgacg ccgagcggca gcagcggcag cctgtccacg
      241 tcgtcgtcgt ccagcccgcc cggcacgccg agccccgccg acgccaaggc cgcgcccgcc
      301 gcctgcttcg cggggccgcc ggccgcgccc gccaaggcca aggccaagaa gacggtggac
      361 aagctgagcg acgagtacaa gatgcggcgc gagcgcaaca acatcgcggt gcgcaagagc
      421 cgcgacaagg ccaagatgcg caacctggag acgcagcaca aggtgctgga gctgacggcg
      481 gagaacgagc ggctgcagaa gaaggtggag cagctgtcgc gagagctcag caccctgcgg
      541 aacttgttca agcagctgcc cgagccgctg ctggcctcgg cgggccactg ctaggtcgac
      601 ggatccgagc tcggtaccaa gcttaagttt aaaccgctga tcagcctcga ctgtgccttc
      661 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
      721 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
      781 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
      841 ta
//
