LOCUS       sequence-76260-e         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  regulate protein stability with light.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Bonger KM, Rakhit R, Payumo AY, Chen JK, Wandless TJ
  TITLE     A General Method for Regulating Protein Stability with Light.
  JOURNAL   ACS Chem Biol. 2014 Jan 17;9(1):111-5. doi: 10.1021/cb400755b
  PUBMED    24180414
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "ACS Chem Biol"; date: "2014-01-17- 17"; volume: "9"; issue: "1"; 
            pages: "111-5"
COMMENT     Sequence Label: pBMN HAYFP-LOV24
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             32..58
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(59..61,62..64,65..775)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(104..125)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(365..384)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     712..733
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 catcctctag actgccggat ccgccaccat gtatccgtac gacgtaccgg attatgcgat
       61 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
      121 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
      181 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
      241 cgtgaccacc ttcggctacg gcctgcagtg cttcgcccgc taccccgacc acatgaagca
      301 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
      361 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
      421 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
      481 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
      541 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
      601 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
      661 cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
      721 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagacgcg
      781 tttcttggct actacacttg aacgtattga gaagaacttt gtcattactg acccaagatt
      841 gccagataat cccattatat tcgcgtccga tagtttcttg cagttgacag aatatagccg
      901 tgaagaaatt ttgggaagaa actgcaggtt tctacaaggt cctgaaactg atcgcgcgac
      961 agtgagaaaa atta
//
