LOCUS       sequence-76261-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 hCas9 module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Belhaj K, Chaparro-Garcia A, Kamoun S, Nekrasov V
  TITLE     Plant genome editing made easy: targeted mutagenesis in model and 
            crop plants using the CRISPR/Cas system.
  JOURNAL   Plant Methods. 2013 Oct 11;9(1):39.
  PUBMED    24112467
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2013-10-11"; volume: "9(1)"; pages: "39"
COMMENT     Sequence Label: pICH41308::hCas9
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..35)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggtctcaaag ctcacacctt cctcttcttc ttggggtcag ccctgctgtc tccaccgagc
       61 tgagagaggt cgattcttgt ttcatagagc cccgtaattg actgatgaat cagtgtggcg
      121 tccaggacct cctttgtaga ggtgtaccgc tttctgtcta tggtggtgtc gaagtacttg
      181 aaggctgcag gcgcgcccaa gttggtcaga gtaaacaagt ggataatgtt ttctgcctgc
      241 tccctgatgg gcttatccct gtgcttattg taagcagaaa gcaccttatc gaggttagcg
      301 tcggcgagga tcactctttt ggagaattcg cttatttgct cgatgatctc atcaaggtag
      361 tgtttgtgtt gttccacgaa cagctgcttc tgctcattat cttcgggaga tcctttgagc
      421 ttttcatagt ggctggccag atacaagaaa ttaacgtatt tagagggcag tgccagctcg
      481 ttacctttct gcagctcgcc cgcactagcg agcattcgtt tccggccgtt ttcaagctca
      541 aagagagagt acttgggaag cttaatgatg aggtcttttt tgacctcttt atatcctttc
      601 gcctcgagaa agtcgatggg gtttttttcg aagcttgatc gctccatgat tgtgatgccc
      661 agcagttcct tgacgctttt gagtttttta gacttccctt tctccacttt ggccacaacc
      721 agtacactgt aagcgactgt aggagaatcg aatccgccgt atttcttggg gtcccaatct
      781 tttttgcgtg cgatcagctt gtcgctgttc cttttcggga ggatactttc cttggagaag
      841 cctccggtct gtacttcggt ctttttaacg atgttcacct gcggcatgga caggaccttc
      901 cggactgtcg cgaaatccct acccttgtcc cacacgattt ctcctgtttc tccgtttgtt
      961 tcgataag
//
