LOCUS       sequence-76263-e         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Belhaj K, Chaparro-Garcia A, Kamoun S, Nekrasov V
  TITLE     Plant genome editing made easy: targeted mutagenesis in model and 
            crop plants using the CRISPR/Cas system.
  JOURNAL   Plant Methods. 2013 Oct 11;9(1):39.
  PUBMED    24112467
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2013-10-11"; volume: "9(1)"; pages: "39"
COMMENT     Sequence Label: 
            pAGM4723::AtU6p::sgRNA2-2x35S-5′UTR::Cas9::NOST-AtU6p::sgRNA1
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tggagaggac acgctcgagt ataagagctc atttttacaa caattaccaa caacaacaaa
       61 caacaaacaa cattacaatt acatttacaa ttatcgatac aatggacaag aagtactcca
      121 ttgggctcga tatcggcaca aacagcgtcg gctgggccgt cattacggac gagtacaagg
      181 tgccgagcaa aaaattcaaa gttctgggca ataccgatcg ccacagcata aagaagaacc
      241 tcattggcgc cctcctgttc gactccgggg agacggccga agccacgcgg ctcaaaagaa
      301 cagcacggcg cagatatacc cgcagaaaga atcggatctg ctacctgcag gagatcttta
      361 gtaatgagat ggctaaggtg gatgactctt tcttccatag gctggaggag tcctttttgg
      421 tggaggagga taaaaagcac gagcgccacc caatctttgg caatatcgtg gacgaggtgg
      481 cgtaccatga aaagtaccca accatatatc atctgaggaa gaagcttgta gacagtactg
      541 ataaggctga cttgcggttg atctatctcg cgctggcgca tatgatcaaa tttcggggac
      601 acttcctcat cgagggggac ctgaacccag acaacagcga tgtcgacaaa ctctttatcc
      661 aactggttca gacttacaat cagcttttcg aagagaaccc gatcaacgca tccggagttg
      721 acgccaaagc aatcctgagc gctaggctgt ccaaatcccg gcggctcgaa aacctcatcg
      781 cacagctccc tggggagaag aagaacggcc tgtttggtaa tcttatcgcc ctgtcactcg
      841 ggctgacccc caactttaaa tctaacttcg acctggccga agatgccaag cttcaactga
      901 gcaaagacac ctacgatgat gatctcgaca atctgctggc ccagatcggc ga
//
