LOCUS       sequence-76265-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral Gateway destination vector, expression, PGK 
            promoter, Neo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti PGK Neo DEST (w531-1)
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      35..170
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    210..331
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(221..240)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     275..294
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             403..636
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(838..857)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 cgactcacta tagggcgaat tgggtacgtc cctaggcctc caaaaaagcc tcctcactac
       61 ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt
      121 cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt
      181 aggggcggga tagctagagc cagacatgat aagatacatt gatgagtttg gacaaaccac
      241 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
      301 tgtaaccatt ataagctgca ataaacaagt tcctctcact ctctgatatt catttctttg
      361 caagttataa atactgaata ataagatgac atgaactact actgctagag attttccaca
      421 ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca
      481 ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc
      541 cagagagctc ccaggctcag atctggtcta accagagaga cccagtacaa gcaaaaagca
      601 gatcttgtct tcgttgggag tgagctagcc cttccagtcc ccccttttct tttaaaaagt
      661 ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa aggtaccggg ccctcgacgg
      721 atcccctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc
      781 ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat
      841 atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc
      901 gatgaatcca gaaaagcggc cattttccca ccatgatatt cggcaagcag
//
