LOCUS       sequence-76268-e         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSV40mod-luc2 donor plasmid for MPRA library construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Melnikov A, Zhang X, Rogov P, Wang L, Mikkelsen TS
  TITLE     Massively parallel reporter assays in cultured Mammalian cells.
  JOURNAL   J Vis Exp. 2014 Aug 17;(90). doi: 10.3791/51719.
  PUBMED    25177895
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Vis 
            Exp."; date: "2014-08-17"; volume: "(90). doi"; pages: " 
            10.3791/51719"
COMMENT     Sequence Label: pMPRAdonor3
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..58
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     50..67
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     51..67
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(83..99)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(83..99)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(96..118)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    107..123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(131..137,138..155,156..161))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 gctgcaggcg attagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
       61 ggccagtgaa tccgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
      121 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
      181 tgagctaact tacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
      241 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
      301 gccagggtgg tttttctttt caccagtgag acgggcaaca gctgattgcc cttcaccgcc
      361 tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag gcgaaaatcc
      421 tgtttgatgg tggttaacgg cgggatataa catgagctgt cttcggtatc gtcgtatccc
      481 actaccgaga tatccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc
      541 agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc attcagcatt
      601 tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc cgctatcggc
      661 tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca
      721 gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc
      781 acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg tgtctggtca
      841 gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc aatggcatcc
      901 tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc
      961 accgccgctt tacaggcttc gacgccgctt
//
