LOCUS       sequence-76276-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Basic backbone for transient transfection of Massively Parallel 
            Reporter Assay (MPRA) libraries.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Melnikov A, Zhang X, Rogov P, Wang L, Mikkelsen TS
  TITLE     Massively parallel reporter assays in cultured Mammalian cells.
  JOURNAL   J Vis Exp. 2014 Aug 17;(90). doi: 10.3791/51719.
  PUBMED    25177895
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Vis 
            Exp."; date: "2014-08-17"; volume: "(90). doi"; pages: " 
            10.3791/51719"
COMMENT     Sequence Label: pMPRA1
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(213..230)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(464..483)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat
       61 cgataaggat ccgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt
      121 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg
      181 taggacaggt gccggcagcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
      241 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      301 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      361 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      421 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      481 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      541 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      601 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      661 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
      721 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
      781 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
      841 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
      901 caaaccaccg ctggtagcg
//
