LOCUS       sequence-76284-e         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses EGFP-Rab22 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  TITLE     Scidmore Lab GFP-Rab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: EGFP-Rab22
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             265..276
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      650..659
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      654..663
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    817..938
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(854..873)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     908..927
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ctctcggcat ggacgagctg tacaagtccg gactcagatc catggcgctg agggagctca
       61 aagtgtgtct gctcggggat acaggtgtag gtaaatcgag tattgtgtgg cggtttgtgg
      121 aagacagttt tgatccaaac atcaacccaa caataggggc atcttttatg accaagactg
      181 tccagtacca aaatgagcta cataaattcc taatctggga tacagctgga caagaacgat
      241 ttcgtgcctt agcaccaatg tactatcgag ggtcggctgc agctataatc gtttatgata
      301 tcacaaaaga agagacattt tcaacattaa agaattgggt gaaagagctt cgacagcatg
      361 gcccacctaa tattgtagtt gccattgcag gaaataaatg tgatcttatc gatgtaagag
      421 aagtcatgga gagagatgca aaggactacg ccgactctat tcatgcaatt tttgtagaga
      481 ccagcgcaaa aaacgcgata aacataaatg aactctttat agaaattagt cgaagaattc
      541 catccactga cgccaacctg ccatctggcg gtaagggctt caaactccga agacagcctt
      601 cagagccaaa gcggagctgc tgctgagtca gcggaatcga attcccgcgg ccgccatggc
      661 ggccgggagc atgcgacgtc gggcccggga tccaccggat ctagataact gatcataatc
      721 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
      781 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
      841 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
      901 tctagttgtg gtttgtccaa actcatcaat gtatcttaac gcgtaaattg taagcgttaa
      961 tattttgtta aaattcgcgt taaatttttg
//
