LOCUS       sequence-76286-e       10148 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for membrane PIP3 levels.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10148)
  AUTHORS   Toettcher JE, Gong D, Lim WA, Weiner OD
  TITLE     Light-based feedback for controlling intracellular signaling 
            dynamics.
  JOURNAL   Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700.
  PUBMED    21909100
REFERENCE   2  (bases 1 to 10148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-09-11- 11"; volume: "8"; 
            issue: "10"; pages: "837-9"
COMMENT     Sequence Label: pHR SFFVp PHAkt Cerulean
FEATURES             Location/Qualifiers
     source          1..10148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    324..441
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        595..1003
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             join(1559..1561,1562..1564,1565..2278)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1604..1625)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1865..1884)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2212..2233
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2338..2926
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2391..2411)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2809..2820)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2989..3222
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3247..3265)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3248..3265)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3555..3574)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     3908..3930
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3968..3986)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4054..4158
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4159..4227,4228..5019)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4377..4396)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5190..5778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5679..5698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5932..5949
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6019..6039)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6024..6353
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6204..6339
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7487..7552
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7682..7702
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8127..8261
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8164..8183)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8218..8237
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8430..9063
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9110..9235
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9727..9960
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             join(10145..10148,1..41)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
       61 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
      121 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
      181 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
      241 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtcgc
      301 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
      361 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
      421 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggatc
      481 gataagcttg atatcgaatt cctgcagccc cgataaaata aaagatttta tttagtctcc
      541 agaaaaaggg gggaatgaaa gaccccacct gtaggttatg gcaagctagc tgcagtaacg
      601 ccattattgc aaggcatgga aaaataccaa accaagaata gagaagttca gatcaagggc
      661 gggtacatga aaatagctaa cgtagggcca aacaggatat ctgcggtgag cagtttcggc
      721 cccggcccgg ggccaagaac agatggtcac cgcagtttcg gccccggccc gaggccaaga
      781 gcagatggtc cccagatatg gcccaaccct cagcagtttc ttaagaccca tcagatgttt
      841 ccaggctccc ccaaggacct gaaatgaccc tgcgccttat ttgaattaac caatcagcct
      901 gcttctcgct tctgttcgcg cgcttctgct tcccgagctc tataaaagag ctcacaaccc
      961 ctcactcggc gcgccagtcc tccgacagac tgagtcgccc gggggggatc tggagctctc
     1021 gagaattctc acgcgtgcca ccatgagcga cgtggctatt gtgaaggagg gttggctgca
     1081 caaacgaggg gagtacatca agacctggcg gccacgctac ttcctcctca agaatgatgg
     1141 caccttcatt ggctacaagg agcggccgca ggatgtggac caacgtgagg ctcccctcaa
     1201 caacttctct gtggcgcagt gccagctgat gaagacggag cggccccggc ccaacacctt
     1261 catcatccgc tgcctgcagt ggaccactgt catcgaacgc accttccatg tggagactcc
     1321 tgaggagcgg gaggagtgga caaccgccat ccagactgtg gctgacggcc tcaagaagca
     1381 ggaggaggag gagatggact tccggtcggg ctcacccagt gacaactcag gggctgaaga
     1441 gatggaggtg tccctggcca agcccaagca ccgcgtgacc atgaacgagt ttgagtacct
     1501 gaagctgctg ggcaagggca ctttcggcaa ggtacctagc ggccatatgg ggccaagaat
     1561 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1621 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1681 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1741 cgtgaccacc ctgacctggg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca
     1801 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1861 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1921 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1981 gctggagtac aacgccatca gcgacaacgt ctatatcacc gccgacaagc agaagaacgg
     2041 catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     2101 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     2161 cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     2221 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagc
     2281 ggccgcgact ctagagtcga cctgcaggca tgcaagcttg atatcaagct tatcgataat
     2341 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     2401 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     2461 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     2521 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     2581 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     2641 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     2701 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     2761 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     2821 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     2881 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga
     2941 ccaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     3001 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     3061 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     3121 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     3181 gatccctcag acccttttag tcagtgtgga aaatctctag cagcatctag aattaattcc
     3241 gtgtattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt
     3301 gtagccgcgt tctaacgaca atatgtacaa gcctaattgt gtagcatctg gcttactgaa
     3361 gcagacccta tcatctctct cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt
     3421 ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt
     3481 catcgctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
     3541 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt
     3601 cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact
     3661 gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa
     3721 cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaatggtgca
     3781 ctctcagtac aatctagctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     3841 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     3901 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     3961 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     4021 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     4081 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     4141 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     4201 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     4261 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     4321 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     4381 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     4441 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     4501 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     4561 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     4621 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     4681 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     4741 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     4801 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     4861 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     4921 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     4981 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     5041 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     5101 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     5161 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5221 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5281 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     5341 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5401 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     5461 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     5521 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     5581 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     5641 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     5701 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     5761 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     5821 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     5881 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     5941 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     6001 attcattaat gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     6061 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     6121 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     6181 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     6241 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     6301 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg
     6361 gacacaagac aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga
     6421 ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc
     6481 tataatctcg cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct
     6541 gggacacttc acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca
     6601 cgtaaactcg caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc
     6661 cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata
     6721 ccgtttgtat ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg
     6781 cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg
     6841 cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg
     6901 ctggtcgtcc gctggttgat gactatgttg ttgatatccc gcaagatacc tggattgaac
     6961 agccgtggga tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac
     7021 gcctggcact gccgggcgtt gttcttttta acttcaggcg ggttacaata gtttccagta
     7081 agtattctgg aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc
     7141 cgctttaaac atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg
     7201 cctgtgcagt cggcccttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt
     7261 ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt
     7321 gtgatgagcg atgccgaacg taccgacgat gatttatacg atacggtgat tggctaccgt
     7381 ggcggcaact ggatttatga gtgggccccg gatctttgtg aaggaacctt acttctgtgg
     7441 tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt
     7501 tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac
     7561 ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg
     7621 ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc
     7681 tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt
     7741 tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa
     7801 ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag
     7861 taggcataac agttataatc ataacatact gttttttctt actccacaca ggcatagagt
     7921 gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg
     7981 ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca
     8041 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     8101 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     8161 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     8221 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
     8281 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctagtgg
     8341 tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt
     8401 tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc aaagaagaca
     8461 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta
     8521 cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc
     8581 agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc
     8641 tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag
     8701 ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg
     8761 atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg
     8821 cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta
     8881 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     8941 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     9001 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     9061 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     9121 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     9181 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     9241 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     9301 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     9361 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     9421 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     9481 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     9541 gcaaaacaaa agtaagacca ccgcacagca agcggccggt gatcttcaga cctggacgat
     9601 atatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     9661 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     9721 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     9781 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     9841 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     9901 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     9961 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
    10021 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
    10081 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
    10141 agaaaaga
//
