LOCUS       sequence-76291-e       12529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Optogenetic PI3K activation system; membrane component.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12529)
  AUTHORS   Toettcher JE, Gong D, Lim WA, Weiner OD
  TITLE     Light-based feedback for controlling intracellular signaling 
            dynamics.
  JOURNAL   Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700.
  PUBMED    21909100
REFERENCE   2  (bases 1 to 12529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-09-11- 11"; volume: "8"; 
            issue: "10"; pages: "837-9"
COMMENT     Sequence Label: pHR SFFVp Phy-mCherry-CAAX
FEATURES             Location/Qualifiers
     source          1..12529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    92..324
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             509..553
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    838..955
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1108..1515
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      1549..1558
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5181..5769
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5234..5254)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5652..5663)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5772..5788)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5773..5789)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5879..6112
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6137..6155)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6138..6155)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6445..6464)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6678..6697)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6797..6819
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6857..6875)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6943..7047
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7048..7116,7117..7908)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7266..7285)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8079..8667
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8568..8587
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8821..8838
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8908..8928)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8913..9242
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9093..9228
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10376..10441
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10571..10591
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11016..11150
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11053..11072)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11107..11126
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             11319..11952
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11999..12124
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
       61 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
      121 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
      181 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
      241 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtgaaag
      301 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
      361 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
      421 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
      481 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
      541 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
      601 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
      661 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
      721 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
      781 cattcgatta gtgaacggat ctcgacggta tcgccaaatg gcagtattca tccacaattt
      841 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
      901 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
      961 ttattacagg gacagcagag atccagtttg gatcgataag cttgatatcg aattcctgca
     1021 gccccgataa aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc
     1081 acctgtaggt ttggcaagct agctgcagta acgccatttt gcaaggcatg gaaaaatacc
     1141 aaaccaagaa tagagaagtt cagatcaagg gcgggtacat gaaaatagct aacgttgggc
     1201 caaacaggat atctgcggtg agcagtttcg gccccggccc ggggccaaga acagatggtc
     1261 accgcagttt cggccccggc ccgaggccaa gaacagatgg tccccagata tggcccaacc
     1321 ctcagcagtt tcttaagacc catcagatgt ttccaggctc ccccaaggac ctgaaatgac
     1381 cctgcgcctt atttgaatta accaatcagc ctgcttctcg cttctgttcg cgcgcttctg
     1441 cttcccgagc tctataaaag agctcacaac ccctcactcg gcgcgccagt cctccgacag
     1501 actgagtcgc ccggggggga tctggagctc tcgagaattc tcacgcgtgc caccatggta
     1561 tcaggtgttg gaggaagtgg tggaggtaga ggtggaggaa gaggtggaga agaagagccc
     1621 tcaagttcac acactcccaa caatagacga gggggtgaac aagcgcagtc ttctggtacg
     1681 aagtccctcc ggcctcgctc taatacagaa tccatgtcca aagccatcca gcagtataca
     1741 gtggatgcca ggctccacgc agtttttgaa cagagcgggg aaagtgggaa atctttcgat
     1801 tatagccaga gtctgaagac cacaacttac ggcagcagtg ttccagagca acagattacg
     1861 gcttacctta gccggataca gcgcggaggc tacatacagc cttttgggtg tatgatcgcg
     1921 gttgacgaat ctagcttccg cataatcgga tattctgaaa acgctcggga gatgctgggt
     1981 atcatgccac agagcgttcc tacactggaa aagcctgaaa tcctcgcgat gggcaccgat
     2041 gtgcgcagcc tcttcacgtc atcttcaagt atcctcctgg agcgcgcttt tgttgcccgg
     2101 gaaatcacac ttctgaatcc tgtctggatt catagcaaga atacagggaa acccttttac
     2161 gctatcctgc acagaatcga tgtcggggtt gtaatagacc ttgagccagc tagaacagaa
     2221 gatccggcct tgagcatcgc tggcgcagtg caaagccaga agctggcagt gagggcaata
     2281 tctcaactgc aagccctgcc aggcggcgat ataaagctgc tgtgtgatac cgtagtggag
     2341 agcgttcgcg atctgaccgg ttatgacaga gtgatggtct acaaatttca tgaagatgag
     2401 catggggagg tagtggcaga atcaaaaaga gacgacttgg aaccctatat cggactccac
     2461 tatcctgcta ctgatattcc ccaggcaagt cggtttttgt ttaagcagaa tagggtcagg
     2521 atgattgtgg actgtaatgc tactcccgtg ttggtagtcc aagacgatcg actgacccaa
     2581 tctatgtgcc tggtggggag tacactgcgc gcaccacacg ggtgccattc ccagtatatg
     2641 gcgaacatgg ggagcatcgc ctcactggca atggctgtaa tcatcaacgg taacgaggat
     2701 gatggatcta atgtcgccag tggcagatcc tcaatgcgcc tctgggggct ggtcgtctgc
     2761 catcatacaa gcagccggtg tattcccttt ccgctgaggt acgcttgtga gtttcttatg
     2821 caagcatttg ggcttcagct taatatggaa ctccagcttg cccttcaaat gagtgagaag
     2881 cgggttctcc gcactcagac ccttctctgc gatatgcttt tgcgcgattc ccccgctggc
     2941 atcgtgaccc agagcccttc aataatggac cttgtaaagt gcgatggtgc tgcattcctg
     3001 tatcatggga aatactatcc actgggagtg gccccaagcg aagtacagat aaaggacgtg
     3061 gtcgagtggc tgctcgccaa tcacgccgac agtacagggc tgagcaccga tagtctgggg
     3121 gacgccggat atccaggtgc cgccgcactt ggcgatgcag tgtgtggcat ggcagtcgcc
     3181 tatattacga agcgcgattt tctcttttgg tttcgatcac acacagcaaa agagataaag
     3241 tggggaggcg ccaagcacca tccagaggat aaggatgacg ggcaaagaat gcatccaaga
     3301 agttctttcc aagccttcct cgaagtggtt aagtcacgat cacagccttg ggaaaccgcc
     3361 gaaatggacg ctatccattc tctccagctg atcctgcggg acagtttcaa ggagagcgaa
     3421 gctgctatga atagcaaggt cgtcgatggt gtggttcagc cttgccgcga tatggcaggc
     3481 gagcagggga ttgacgagct gggcgctgtg gcacgcgaga tggttcggct gattgaaact
     3541 gctactgtgc ccatcttcgc agtagatgca ggcggctgca ttaatggatg gaacgccaag
     3601 atcgccgaat tgactggact gtctgtcgag gaagccatgg gtaagagtct cgtgtccgac
     3661 ctgatctaca aggagaacga ggcaaccgtc aacaaactcc tgagtcgcgc tttgaggggt
     3721 gacgaggaga agaatgtaga agtcaagctc aaaaccttca gccctgagct gcaaggaaaa
     3781 gctgtatttg tcgtcgtgaa tgcctgctct tccaaggact atctgaataa catcgtaggg
     3841 gtgtgcttcg tgggccagga tgtaacctcc cagaagatag tgatggacaa attcatcaat
     3901 atacaggggg attataaagc cattgtccac agccctaacc ccctgatccc tccaattttt
     3961 gctgctgacg agaatacatg ctgcctcgaa tggaatatgg cgatggagaa gctcactgga
     4021 tggtcccgca gcgaggtaat cggaaagatg atcgtcggtg aggtgtttgg gtcttgctgc
     4081 atgctgaagg gcccagacgc attgaccaaa tttatgatcg ttctgcataa tgcaatcggg
     4141 ggccaggaca ccgacaaatt cccattcccc tttttcgata ggaatggcaa atttgtgcag
     4201 gcactcctca ccgccaacaa acgagtatca ctcgaaggga aggtgatcgg cgcattctgt
     4261 ttcttgcaga tcccgtcccc cgaattgcag caagcgctcg ccgtcgctag cgattccgca
     4321 ggctctgctg gctcagcagg cctgcaggtg tctaaaggcg aggaggataa catggctata
     4381 atcaaggaat ttatgaggtt caaggtgcat atggagggat ctgtgaacgg tcacgagttc
     4441 gaaatcgagg gcgaaggcga ggggcgaccc tatgaaggga cacagacggc caaactgaag
     4501 gtgaccaagg gtggccccct gcctttcgcc tgggacatcc tgtcacccca gtttatgtac
     4561 ggctctaagg cttacgttaa gcaccctgcc gatattccgg actacttgaa actgagcttc
     4621 cctgaagggt tcaagtggga acgggtaatg aattttgagg atggcggtgt agtcacagtt
     4681 acccaggaca gctctcttca ggacggagaa tttatctata aggttaaact tcggggcact
     4741 aacttcccat cagacggccc cgtgatgcag aagaaaacta tgggctggga agccagctca
     4801 gaacgcatgt atcccgagga cggagcactg aagggagaaa tcaagcagcg actcaagctg
     4861 aaggatgggg gacattatga tgctgaggtc aaaaccacct ataaggccaa gaaacccgtt
     4921 cagcttcctg gggcatacaa cgtgaatatc aaactggata ttacatccca taacgaagac
     4981 tataccatcg tggaacagta cgagcgggcc gagggcagac atagcacagg gggcatggat
     5041 gaattgtaca agctcgagaa aatgtccaag gatggtaaga aaaagaagaa gaagtcaaaa
     5101 accaagtgtg ttatcatgta agcggccgcg actctagagt cgacctgcag gcatgcaagc
     5161 ttgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     5221 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     5281 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     5341 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     5401 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     5461 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     5521 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     5581 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     5641 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     5701 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     5761 cctccccgca tcgataccgt cgacctcgag ggaattaatt cgagctcggt acctttaaga
     5821 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     5881 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     5941 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6001 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6061 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagcatctag
     6121 aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta
     6181 tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt gtagcatctg
     6241 gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc ggtttggttg
     6301 gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa
     6361 tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg
     6421 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
     6481 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
     6541 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
     6601 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
     6661 gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     6721 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     6781 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     6841 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     6901 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     6961 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     7021 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     7081 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     7141 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     7201 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     7261 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     7321 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     7381 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     7441 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     7501 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     7561 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     7621 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     7681 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     7741 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     7801 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7861 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     7921 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     7981 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     8041 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     8101 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     8161 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     8221 actgtctttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     8281 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     8341 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     8401 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     8461 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     8521 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     8581 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     8641 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     8701 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     8761 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     8821 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     8881 cgttggccga ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     8941 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     9001 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     9061 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     9121 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     9181 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     9241 aaaagcttgg acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg
     9301 tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg
     9361 ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat
     9421 aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag
     9481 atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt
     9541 gccgtaagcc gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc
     9601 atgtcgatac cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc
     9661 tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg
     9721 gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg
     9781 caaaaccggc tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct
     9841 ggattgaaca gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat
     9901 cttttcaacg cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag
     9961 tttccagtaa gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg
    10021 ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc
    10081 gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg
    10141 attaaccgtc tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag
    10201 gcacgtattg tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt
    10261 ggctaccgtg gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta
    10321 cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa
    10381 tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta
    10441 gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa
    10501 cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca
    10561 ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt
    10621 gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta
    10681 caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac
    10741 ctttataagt aggcataaca gttataatca taacatactg ttttttctta ctccacacag
    10801 gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat
    10861 ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca
    10921 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
    10981 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
    11041 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    11101 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
    11161 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
    11221 acctagtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
    11281 aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca
    11341 aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta
    11401 gcagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa
    11461 gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt
    11521 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag
    11581 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa
    11641 gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg
    11701 tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt
    11761 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
    11821 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
    11881 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
    11941 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
    12001 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
    12061 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
    12121 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
    12181 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
    12241 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
    12301 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
    12361 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
    12421 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc
    12481 agacctggag gaggagatat gagggacaat tggagaagtg aattatata
//
