LOCUS       sequence-76296-e       10776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for Erk nuclear translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10776)
  AUTHORS   Toettcher JE, Weiner OD, Lim WA
  TITLE     Using optogenetics to interrogate the dynamic control of signal 
            transmission by the ras/erk module.
  JOURNAL   Cell. 2013 Dec 5;155(6):1422-34. doi: 10.1016/j.cell.2013.11.004.
  PUBMED    24315106
REFERENCE   2  (bases 1 to 10776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.11"; journalName: "Cell"; date: "2013-12-5- 5";
            volume: "155"; issue: "6"; pages: "1422-34"
COMMENT     Sequence Label: pHR SFFVp BFP-Erk2
FEATURES             Location/Qualifiers
     source          1..10776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    19..252
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             437..481
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    764..881
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1035..1443
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1518..2216
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             3270..3305
                     /codon_start=1
                     /product="epitope tag derived from MAP kinase 2"
                     /label=Tag-100
                     /translation="EETARFQPGYRS"
     misc_feature    3406..3994
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3459..3479)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3877..3888)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4057..4290
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4315..4333)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4316..4333)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4623..4642)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4976..4998
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5036..5054)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5122..5226
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5227..5295,5296..6087)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5445..5464)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6258..6846
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6747..6766
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7000..7017
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7087..7107)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7092..7421
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7272..7407
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8555..8620
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8750..8770
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9195..9329
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9232..9251)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9286..9305
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9498..10131
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10178..10303
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
       61 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
      121 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
      181 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
      241 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
      301 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
      361 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
      421 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
      481 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
      541 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
      601 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
      661 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
      721 gaacggatct cgacggtcgc caaatggcag tattcatcca caattttaaa agaaaagggg
      781 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
      841 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
      901 gcagagatcc agtttggatc gataagcttg atatcgaatt cctgcagccc cgataaaata
      961 aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggttatg
     1021 gcaagctagc tgcagtaacg ccattattgc aaggcatgga aaaataccaa accaagaata
     1081 gagaagttca gatcaagggc gggtacatga aaatagctaa cgtagggcca aacaggatat
     1141 ctgcggtgag cagtttcggc cccggcccgg ggccaagaac agatggtcac cgcagtttcg
     1201 gccccggccc gaggccaaga gcagatggtc cccagatatg gcccaaccct cagcagtttc
     1261 ttaagaccca tcagatgttt ccaggctccc ccaaggacct gaaatgaccc tgcgccttat
     1321 ttgaattaac caatcagcct gcttctcgct tctgttcgcg cgcttctgct tcccgagctc
     1381 tataaaagag ctcacaaccc ctcactcggc gcgccagtcc tccgacagac tgagtcgccc
     1441 gggggggatc tggagctctc gagaattctc acgcgtcaag tggagcaagg caggtggaca
     1501 gtggatccgg agctaccatg agcgagctga ttaaggagaa catgcacatg aagctgtaca
     1561 tggagggcac cgtggacaac catcacttca agtgcacatc cgagggcgaa ggcaagccct
     1621 acgagggcac ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct
     1681 tcgacatcct ggctactagc ttcctctacg gcagcaagac cttcatcaac cacacccagg
     1741 gcatccccga cttcttcaag cagtccttcc ctgagggctt cacatgggag agagtcacca
     1801 catacgaaga cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc
     1861 tcatctacaa cgtcaagatc agaggggtga acttcacatc caacggccct gtgatgcaga
     1921 agaaaacact cggctgggag gccttcaccg agacgctgta ccccgctgac ggcggcctgg
     1981 aaggcagaaa cgacatggcc ctgaagctcg tgggcgggag ccatctgatc gcaaacatca
     2041 agaccacata tagatccaag aaacccgcta agaacctcaa gatgcctggc gtctactatg
     2101 tggactacag actggaaaga atcaaggagg ccaacaacga gacctacgtc gagcagcacg
     2161 aggtggcagt ggccagatac tgcgacctcc ctagcaaact ggggcacaag cttaatgctc
     2221 aagcttcgaa ttctgcagtc gacggtaccg cgggcccgga gatggtccgc gggcaggtgt
     2281 tcgacgtagg gccgcgctac accaacctct cgtacatcgg agaaggcgcc tacggcatgg
     2341 tttgctctgc ttatgataat ctcaacaaag ttcgagttgc tatcaagaaa atcagtcctt
     2401 ttgagcacca gacctactgt caaagaaccc taagagagat aaaaatctta ctgcgcttca
     2461 gacatgagaa catcattggc atcaatgaca tcatccgggc accaaccatt gagcaaatga
     2521 aagatgtata tatagtacag gacctcatgg agacggacct ttacaagctc ttgaagacac
     2581 agcacctcag caatgaccac atctgctatt ttctttatca gatcctgaga gggctaaagt
     2641 atatccattc agctaacgtt ctgcaccgtg acctcaagcc ttccaacctc ctgctgaaca
     2701 ccacttgtga tctcaagatc tgtgactttg gccttgcccg tgttgcagat ccagatcatg
     2761 atcacacagg gttcttgaca gagtacgtag ccacacgttg gtacagagct ccagaaatta
     2821 tgttgaattc caagggttat accaagtcca ttgatatttg gtctgtgggc tgcatcctgg
     2881 cagagatgct atccaacagg cctatcttcc caggaaagca ttaccttgac cagctgaatc
     2941 acatcctggg tattcttgga tctccatcac aggaagatct gaattgtata ataaatttaa
     3001 aagctagaaa ctatttgctt tctctcccgc acaaaaataa ggtgccatgg aacaggttgt
     3061 tcccaaatgc tgactccaaa gctctggatt tactggataa aatgttgaca tttaaccctc
     3121 acaagaggat tgaagttgaa caggctctgg cccacccata cctggagcag tattatgacc
     3181 caagtgatga gcccattgct gaagcgccat tcaagtttga catggagttg gacgacttac
     3241 ctaaggagaa gctcaaagaa ctcatttttg aagagactgc tagattccag ccaggataca
     3301 gatcttaagc ggccgcaact cccacctgca acatgcgtga ctgactgagg ccgcgactct
     3361 agagtcgacc tgcaggcatg caagcttgat atcaagctta tcgataatca acctctggat
     3421 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     3481 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     3541 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     3601 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     3661 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     3721 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     3781 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     3841 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     3901 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     3961 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc aaggcagctg
     4021 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     4081 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     4141 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     4201 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     4261 ccttttagtc agtgtggaaa atctctagca gcatctagaa ttaattccgt gtattctata
     4321 gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc
     4381 taacgacaat atgtacaagc ctaattgtgt agcatctggc ttactgaagc agaccctatc
     4441 atctctctcg taaactgccg tcagagtcgg tttggttgga cgaaccttct gagtttctgg
     4501 taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc agcagggtca tcgctagcca
     4561 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
     4621 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
     4681 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
     4741 ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg
     4801 ctgcttccta atgcaggagt cgcataaggg agagcgtcga atggtgcact ctcagtacaa
     4861 tctagctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     4921 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     4981 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     5041 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     5101 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     5161 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     5221 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5281 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     5341 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     5401 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5461 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5521 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5581 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5641 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5701 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5761 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5821 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5881 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5941 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     6001 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     6061 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     6121 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6181 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6241 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6301 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6361 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     6421 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6481 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6541 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6601 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6661 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6721 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6781 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6841 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6901 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     6961 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     7021 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     7081 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7141 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     7201 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     7261 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7321 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     7381 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga cacaagacag
     7441 gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa
     7501 aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg
     7561 caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac
     7621 atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca
     7681 agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg
     7741 taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt
     7801 ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg
     7861 gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc
     7921 gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc
     7981 tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag ccgtgggata
     8041 tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc
     8101 cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag
     8161 gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat
     8221 cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg
     8281 gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc
     8341 tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat
     8401 gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg
     8461 atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
     8521 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
     8581 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
     8641 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
     8701 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
     8761 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
     8821 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
     8881 actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag
     8941 ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa
     9001 taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga
     9061 atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg
     9121 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     9181 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     9241 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     9301 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
     9361 ccaaacttcc caccccatac cctattacca ctgccaatta cctagtggtt tcatttactc
     9421 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
     9481 actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga
     9541 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc
     9601 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga
     9661 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca
     9721 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt
     9781 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg
     9841 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga
     9901 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct
     9961 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
    10021 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
    10081 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
    10141 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
    10201 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
    10261 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
    10321 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
    10381 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
    10441 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
    10501 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
    10561 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
    10621 taagaccacc gcacagcaag cggccggtga tcttcagacc tggacgatat atatgaggga
    10681 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
    10741 acccaccaag gcaaagagaa gagtggtgca gagaga
//
