LOCUS       sequence-76298-e       11524 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Optogenetic Ras activation system; cytoplasmic component.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11524)
  AUTHORS   Toettcher JE, Weiner OD, Lim WA
  TITLE     Using optogenetics to interrogate the dynamic control of signal 
            transmission by the ras/erk module.
  JOURNAL   Cell. 2013 Dec 5;155(6):1422-34. doi: 10.1016/j.cell.2013.11.004.
  PUBMED    24315106
REFERENCE   2  (bases 1 to 11524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.11"; journalName: "Cell"; date: "2013-12-5- 5";
            volume: "155"; issue: "6"; pages: "1422-34"
COMMENT     Sequence Label: pHR SFFVp BFP-PIF-SOS2cat
FEATURES             Location/Qualifiers
     source          1..11524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    227..344
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        498..906
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             982..1680
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    3617..4205
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3670..3690)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4088..4099)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4268..4501
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4526..4544)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4527..4544)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4834..4853)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     5187..5209
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5247..5265)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5333..5437
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5438..5506,5507..6298)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5656..5675)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6469..7057
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6958..6977
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7211..7228
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7298..7318)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7303..7632
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7483..7618
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8766..8831
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8961..8981
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9406..9540
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9443..9462)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9497..9516
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9709..10342
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10389..10514
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11006..11239
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11424..11468
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
       61 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
      121 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
      181 agtgaacgga tctcgacggt cgccaaatgg cagtattcat ccacaatttt aaaagaaaag
      241 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
      301 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
      361 acagcagaga tccagtttgg atcgataagc ttgatatcga attcctgcag ccccgataaa
      421 ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt
      481 atggcaagct agctgcagta acgccattat tgcaaggcat ggaaaaatac caaaccaaga
      541 atagagaagt tcagatcaag ggcgggtaca tgaaaatagc taacgtaggg ccaaacagga
      601 tatctgcggt gagcagtttc ggccccggcc cggggccaag aacagatggt caccgcagtt
      661 tcggccccgg cccgaggcca agagcagatg gtccccagat atggcccaac cctcagcagt
      721 ttcttaagac ccatcagatg tttccaggct cccccaagga cctgaaatga ccctgcgcct
      781 tatttgaatt aaccaatcag cctgcttctc gcttctgttc gcgcgcttct gcttcccgag
      841 ctctataaaa gagctcacaa cccctcactc ggcgcgccag tcctccgaca gactgagtcg
      901 cccggggggg atctggagct ctcgagaatt ctcacgcgtc aagtggagca aggcaggtgg
      961 acagtggatc cgcttggtac catgagcgag ctgattaagg agaacatgca catgaagctg
     1021 tacatggagg gcaccgtgga caaccatcac ttcaagtgca catccgaggg cgaaggcaag
     1081 ccctacgagg gcacccagac catgagaatc aaggtggtcg agggcggccc tctccccttc
     1141 gccttcgaca tcctggctac tagcttcctc tacggcagca agaccttcat caaccacacc
     1201 cagggcatcc ccgacttctt caagcagtcc ttccctgagg gcttcacatg ggagagagtc
     1261 accacatacg aagacggggg cgtgctgacc gctacccagg acaccagcct ccaggacggc
     1321 tgcctcatct acaacgtcaa gatcagaggg gtgaacttca catccaacgg ccctgtgatg
     1381 cagaagaaaa cactcggctg ggaggccttc accgagacgc tgtaccccgc tgacggcggc
     1441 ctggaaggca gaaacgacat ggccctgaag ctcgtgggcg ggagccatct gatcgcaaac
     1501 atcaagacca catatagatc caagaaaccc gctaagaacc tcaagatgcc tggcgtctac
     1561 tatgtggact acagactgga aagaatcaag gaggccaaca acgagaccta cgtcgagcag
     1621 cacgaggtgg cagtggccag atactgcgac ctccctagca aactggggca caagcttaat
     1681 ggatccatga tgttcttacc aaccgattat tgttgcaggt taagcgatca agagtatatg
     1741 gagcttgtgt ttgagaatgg ccagattctt gcaaagggcc aaagatccaa cgtttctctg
     1801 cataatcaac gtaccaaatc gatcatggat ttgtatgagg cagagtataa cgaggatttc
     1861 atgaagagta tcatccatgg tggtggtggt gccatcacaa atctcgggga cacgcaggtt
     1921 gttccacaaa gtcatgttgc tgctgcccat gaaacaaaca tgttggaaag caataaacat
     1981 gttgacagtg ctggtggtag tgctggtggt agtgctggtg gtagtgctgg tggtagtgct
     2041 ggtggtgaat tggcggccca accactgaga ttaccaagtc ctgaagtata tcgttttgta
     2101 gtaaaagact ctgaggaaaa cattgttttt gaagacaact tgcaaagtag aagtggcatc
     2161 cccattatta aaggaggaac tgtagtgaaa ttaattgaaa ggttaacata tcatatgtat
     2221 gcagatccca attttgttcg tacttttctt accacatatc gttcattttg taaaccacag
     2281 gaattgctga gcttactgat tgaacggttt gaaattccag agccagaacc tactgacgca
     2341 gacaaattgg caatagagaa aggcgagcag ccaatcagtg cagaccttaa aagatttcgc
     2401 aaggaatatg tccaaccagt acaacttagg atcttaaatg tatttcggca ttgggttgaa
     2461 catcattttt atgactttga aagagacttg gaattgcttg aaagactaga atccttcatt
     2521 tcaagtgtaa gagggaaagc tatgaaaaaa tgggtagagt caattgctaa gatcatcagg
     2581 aggaagaagc aagctcaggc aaacggagta agccataata ttacctttga aagtccacct
     2641 ccaccaattg aatggcatat cagcaaacca ggacagtttg aaacatttga tctcatgaca
     2701 cttcatccaa tagaaattgc acgtcagctg acacttttgg agtctgatct ttacaggaaa
     2761 gttcaaccgt ctgaacttgt agggagtgtg tggaccaaag aagataaaga aataaattct
     2821 ccaaatttat taaaaatgat tcgccatacc acaaatctca ccctctggtt tgaaaaatgc
     2881 attgtggaag cagaaaattt tgaagaacgg gtggcagtac taagtagaat tatagaaatt
     2941 ctgcaagttt ttcaagattt gaataatttc aatggcgtat tggagatagt cagtgcagta
     3001 aattcagtgt cagtatacag actagaccat acctttgagg cactgcagga aaggaaaagg
     3061 aaaattttgg acgaagctgt ggaattaagt caagatcact ttaaaaaata cctagtaaaa
     3121 cttaagtcaa tcaatccacc ttgtgtgcct ttttttggaa tatatttaac aaatattctg
     3181 aagaccgaag aagggaataa tgatttttta aaaaagaaag ggaaagattt aatcaatttc
     3241 agtaagagga ggaaagtagc tgaaattact ggagaaattc agcagtatca gaatcagcct
     3301 tactgtttac ggatagaacc agatatgagg agattctttg aaaaccttaa ccccatggga
     3361 agtgcatctg aaaaagagtt tacagattat ttgttcaaca agtcactaga aattgaacct
     3421 cgaaactgca aacagccacc tcgatttcct aggaaatcaa ctttttcctt aaaatctcct
     3481 ggaataaggc ctaacacagg ccgacatggc tctacctaag cggccgcaac tcccacctgc
     3541 aacatgcgtg actgactgag gccgcgactc tagagtcgac ctgcaggcat gcaagcttga
     3601 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     3661 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     3721 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     3781 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     3841 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     3901 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     3961 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     4021 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     4081 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     4141 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     4201 cccgcatcga taccgtcgac caaggcagct gtagatctta gccacttttt aaaagaaaag
     4261 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     4321 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     4381 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     4441 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     4501 agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg tgtatgatac
     4561 ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg
     4621 tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg
     4681 gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca
     4741 gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc atcgtggccg
     4801 gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg
     4861 aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag
     4921 gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg
     4981 cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg
     5041 gagagcgtcg aatggtgcac tctcagtaca atctagctct gatgccgcat agttaagcca
     5101 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     5161 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     5221 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     5281 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     5341 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     5401 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     5461 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     5521 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     5581 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     5641 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     5701 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     5761 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     5821 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     5881 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     5941 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     6001 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     6061 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     6121 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     6181 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6241 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6301 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     6361 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     6421 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     6481 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6541 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6601 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6661 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6721 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     6781 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     6841 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     6901 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     6961 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7021 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7081 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     7141 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     7201 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     7261 tctccccgcg cgttggccga ttcattaatg cagctgtgga atgtgtgtca gttagggtgt
     7321 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     7381 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7441 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     7501 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     7561 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     7621 ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga gaataccact
     7681 ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt
     7741 ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta
     7801 agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga
     7861 catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa
     7921 aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc gcgtgaactg
     7981 ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa ccagcgcgag
     8041 cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat tgatgacctg
     8101 gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc
     8161 accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt tgatatcccg
     8221 caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc gccaatctcc
     8281 ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa cttcaggcgg
     8341 gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg caaacctgag
     8401 cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag tccgccgctt
     8461 taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg
     8521 gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac gcgaaatcct
     8581 cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg atttatacga
     8641 tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg atctttgtga
     8701 aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct
     8761 ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt
     8821 gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta
     8881 atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg
     8941 actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc
     9001 cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct
     9061 ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat
     9121 attctgtaac ctttataagt aggcataaca gttataatca taacatactg ttttttctta
     9181 ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta
     9241 gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag
     9301 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     9361 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     9421 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     9481 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     9541 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
     9601 actgccaatt acctagtggt ttcatttact ctaaacctgt gattcctctg aattattttc
     9661 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa
     9721 ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     9781 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat
     9841 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga
     9901 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt
     9961 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg
    10021 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc
    10081 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag
    10141 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
    10201 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
    10261 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
    10321 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
    10381 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
    10441 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
    10501 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
    10561 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
    10621 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
    10681 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
    10741 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
    10801 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggtg
    10861 atcttcagac ctggacgata tatatgaggg acaattggag aagtgaatta tataaatata
    10921 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
    10981 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
    11041 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
    11101 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
    11161 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
    11221 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
    11281 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
    11341 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
    11401 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
    11461 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
    11521 tgat
//
