LOCUS       sequence-76303-e        9411 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full length SERA3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9411)
  AUTHORS   Zenonos ZA, Rayner JC, Wright GJ
  TITLE     Towards a comprehensive Plasmodium falciparum merozoite cell surface
            and secreted recombinant protein library.
  JOURNAL   Malar J. 2014 Mar 12;13(1):93. doi: 10.1186/1475-2875-13-93.
  PUBMED    24620899
REFERENCE   2  (bases 1 to 9411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2014-03-12"; volume: "13(1):93. doi"; pages: " 
            10.1186/1475-2875-13-93"
COMMENT     Sequence Label: SERA3-bio-His
FEATURES             Location/Qualifiers
     source          1..9411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4575..4592
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4644..4699
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4698..4717)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6935..6953)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7021..7125
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7126..7194,7195..7986)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7344..7363)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8157..8745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8646..8665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8899..8916
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9033..9054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9069..9074,9075..9092,9093..9099)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9107..9123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9112..9134
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9131..9147
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9131..9147
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcaa
     1201 caacagtgga cgagagcacc aaagagggca gccagaaccc caagaacagc gccagcacaa
     1261 caccagccag cggctctcag aagggcagca gctctgagtc tcctggcagc agcgtggaaa
     1321 agcagagcca ggaaagcaac aaagagtcca ccaacggcgg caacgtggtg tcccagggaa
     1381 cccccgccaa taccttcggc cagaacagca acaaccccgc cgatagccct cagggcacca
     1441 gcacactgcc tagccccccc aagagcatcg acgtgaagtc cgccttcctg aagcactaca
     1501 agggcgtgaa agtgaccggc agctgcaacg ccaacttcca gctgttcctg gtgccccaca
     1561 tcttcatcaa cgtcgagaca aaagagaaca acatccagct cgacgtcaag ttcctcaagc
     1621 tgaccaagcg gatcgacttc gccaaggaca agagcatgct gaagaacaag tgcgagagcg
     1681 gcaagaacca ggccttcaag ttcgtgctgt acttcaagga cgacatcctg accatcaagt
     1741 ggaaggtgta cgaggaaaag agcgccaccc cccagaagtc cgaagagaac accgtggaca
     1801 tcaagctgta caagctgcct aagctggacc agaccatcac cagcatccag gtgcacaccc
     1861 tgagcatcga gggcacaagc tacctgatgg aaagcaagga ctacagcctg ggcaacaacc
     1921 tgcccgagaa gtgcgacgct atcgccagcg actgcttcct gagcggcaac atcaatgtgg
     1981 aaaagtgcct gaagtgcacc ctgaaagtga agaaggtgga agccagcgac gagtgctaca
     2041 aatacgtgtc caaggataag cccaaagaaa caaagctggc cgtgtccggc agcgaagtga
     2101 aagaagtgaa ggccgccagc gtggaccaca gcaacgacaa agagtacgag ctgagccaga
     2161 gcatcaacaa tatcctgaac aagatgtaca agaaagagag caacgacgag aagaacaaca
     2221 aaaaagagct gatcaagctc gaggacgccg acgacagcct gcagaaagaa ctgaacaagt
     2281 actgcaacag cctgaaagag gtggacctga acggcgtgct gagcaagaac gaagtgggca
     2341 acgagaagga cgtgttcaac aatctggcca ccctgctcaa agaacacatg ctggaaagcc
     2401 accatgtggt gttcgagaag ctgaagaata gcgccctgtg cctcaagaac atcgatgact
     2461 ggctgaaaaa caagaacggc ctgatcgtgc cccccagcaa gtataagctg aaggacacca
     2521 atgagaaaaa agaactcaac aacaacgtgg aagtgatcga ggacatgttc aaggccaacg
     2581 agcacggcat cgtggatctg accaagttcc ccatcgacac caactacgcc agctacaagc
     2641 acatcgacca cacctactgt aacaacgact actgcaactg ggccaaagac aagaactcct
     2701 gcatcagcaa gatcaatgtc gaggaccaga agaactgcgc cctgagctgg gccttcgcct
     2761 ccaagtacca cctggaaaca atcaagtgca tgaagggcta tgagcacatc cccatcagca
     2821 gcctgtatat cgccaactgc gccaagaacg agaaaaagga tgtgtgcacc gagggcagca
     2881 accccctgaa ggtgctgcag atgatcgtgg aaaaaggctt cctgcctacc gagggcgact
     2941 acagctacga gcagagcaaa gtgggcgaga catgccccga ggtgcagaac ggatgggtca
     3001 acctgtgggc caacgccaag ctgctggaac agaacaacga tgagcacaac tccctgagca
     3061 ccaagggcta caccgcctac gagagcgagg ccttccagaa agacatgcac agcttcgtga
     3121 agctgattaa ggacgagatc atgaacaagg gcagcgtgat cgcctacgtg aaagccgaca
     3181 agatcatggc ctacgagttc aacggcaaga aggtccagaa cctctgcggc gacaagaccc
     3241 ccgaccacgc cgtgaacatc atcggctacg gcaactacat caacgacgaa caccagaaaa
     3301 agtcctactg gatcgtgcgg aactcctggg gcaagcactg gggcgataag ggccacttca
     3361 aagtggatat gtacggcccc tccgactgcg aggacaactt catccacagc gtggtcatct
     3421 tcaacgtgga cctgcccatc aaccaggaat ccgtcaagaa agaacccaag atctacaact
     3481 actacctgaa ggccagcccc gacttctacc acaacctgta ctacaagaac ttcgacagcc
     3541 agaagggaaa ggccgaccag gccgaaaaca aaaagtctta cctgtacggc caggaagaga
     3601 gcacctccga gcagctgcct agcagcctca gcagccccca gaacaacaag cagagcgagc
     3661 ggagcaaaga aaaggtggac atctttcacg tgctgaagca catcaaggac tccaagatca
     3721 agatgggcat cgtgaagtac gaccacagcg acgccctggg cgaggataac gtgtgcagca
     3781 gaagctacag ctccaacccc gagaagcagg aaggctgcgt caaattctgc aatgagaatt
     3841 ggggcaagtg caaggacgcc gccagccctg gcttttgcct gagcgagctg gaaaagacca
     3901 atgactgctt cttctgctac attggcgcgc cgtcgacctc catcacggcc tataagagtg
     3961 agggggagtc agcggagttc tccttcccac tcaaccttgg agaggaaagc ctgcagggag
     4021 agttgagatg gaaggcagag aaggctcctt cttcccagtc ctggatcacc ttctccctaa
     4081 agaaccaaaa ggtgtctgtg cagaagtcta ctagcaaccc caagttccag ctgtccgaaa
     4141 cgctcccact cacccttcag ataccccagg tctcccttca gtttgctggt tctggcaacc
     4201 tgaccctgac tctggacaga gggatactgt atcaggaagt gaacctggtg gtgatgaaag
     4261 tgactcagcc cgacagcaac actttgacct gtgaggtgat gggacccacc tcacccaaga
     4321 tgagactgat cttgaagcag gagaatcagg aggccagggt ctccaggcag gagaaagtga
     4381 ttcaagtgca ggcccctgaa gcaggggtgt ggcaatgtct actgagtgaa ggtgaagagg
     4441 tcaagatgga ctccaagatc caggttttat ccaaagggtt gaattccggc agcctgcacc
     4501 acatcctgga cgcccagaag atgctgtgga accacaggga caggaacctg cctcctctgg
     4561 cccctctggg cccccaccac catcaccacc attgagtcta gacacgtgat taattaagga
     4621 tcccccgacc tcgacctctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     4681 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt tggtcgagat
     4741 ccctcggaga tctctagcta gaggatcgat ccccgccccg gacgaactaa acctgactac
     4801 gacatctctg ccccttcttc gcggggcagt gcatgtaatc ccttcagttg gttggtacaa
     4861 cttgccaact gggccctgtt ccacatgtga cacggggggg gaccaaacac aaaggggttc
     4921 tctgactgta gttgacatcc ttataaatgg atgtgcacat ttgccaacac tgagtggctt
     4981 tcatcctgga gcagactttg cagtctgtgg actgcaacac aacattgcct ttatgtgtaa
     5041 ctcttggctg aagctcttac accaatgctg ggggacatgt acctcccagg ggcccaggaa
     5101 gactacggga ggctacacca acgtcaatca gaggggcctg tgtagctacc gataagcgga
     5161 ccctcaagag ggcattagca atagtgttta taaggccccc ttgttaaccc taaacgggta
     5221 gcatatgctt cccgggtagt agtatatact atccagacta accctaattc aatagcatat
     5281 gttacccaac gggaagcata tgctatcgaa ttagggttag taaaagggtc ctaaggaaca
     5341 gcgatatctc ccaccccatg agctgtcacg gttttattta catggggtca ggattccacg
     5401 agggtagtga accattttag tcacaagggc agtggctgaa gatcaaggag cgggcagtga
     5461 actctcctga atcttcgcct gcttcttcat tctccttcgt ttagctaata gaataactgc
     5521 tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc
     5581 ccagaataaa atttggacgg ggggttcagt ggtggcattg tgctatgaca ccaatataac
     5641 cctcacaaac cccttgggca ataaatacta gtgtaggaat gaaacattct gaatatcttt
     5701 aacaatagaa atccatgggg tggggacaag ccgtaaagac tggatgtcca tctcacacga
     5761 atttatggct atgggcaaca cataatccta gtgcaatatg atactggggt tattaagatg
     5821 tgtcccaggc agggaccaag acaggtgaac catgttgtta cactctattt gtaacaaggg
     5881 gaaagagagt ggacgccgac agcagcggac tccactggtt gtctctaaca cccccgaaaa
     5941 ttaaacgggg ctccacgcca atggggccca taaacaaaga caagtggcca ctcttttttt
     6001 tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg ttttggactg
     6061 taaaataagg gtgtaataac ttggctgatt gtaaccccgc taaccactgc ggtcaaacca
     6121 cttgcccaca aaaccactaa tggcaccccg gggaatacct gcataagtag gtgggcgggc
     6181 caagataggg gcgcgattgc tgcgatctgg aggacaaatt acacacactt gcgcctgagc
     6241 gccaagcaca gggttgttgg tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa
     6301 tgttgccatg ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta
     6361 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     6421 tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta
     6481 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg
     6541 tatccgggta gcatatgcta tcctaataga gattagggta gtatatgcta tcctaattta
     6601 tatctgggta gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc
     6661 tgggtagcat atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc
     6721 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc
     6781 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     6841 tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct catgataagc
     6901 tgtcaaacat gagaattttc ttgaagacga aagggcctcg tgatacgcct atttttatag
     6961 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     7021 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     7081 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     7141 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     7201 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     7261 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     7321 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     7381 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     7441 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     7501 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     7561 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     7621 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca
     7681 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     7741 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     7801 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     7861 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     7921 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     7981 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     8041 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     8101 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     8161 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     8221 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     8281 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     8341 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     8401 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     8461 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     8521 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     8581 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     8641 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     8701 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     8761 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     8821 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     8881 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     8941 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     9001 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     9061 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     9121 caatttcaca caggaaacag ctatgaccat gattacgcca agctctagct agaggtcgac
     9181 caattctcat gtttgacagc ttatcatcgc agatccgggc aacgttgttg ccattgctgc
     9241 aggcgcagaa ctggtaggta tggaagatct atacattgaa tcaatattgg caattagcca
     9301 tattagtcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt
     9361 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg c
//
