LOCUS       sequence-76304-e        9498 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full length SERA4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9498)
  AUTHORS   Zenonos ZA, Rayner JC, Wright GJ
  TITLE     Towards a comprehensive Plasmodium falciparum merozoite cell surface
            and secreted recombinant protein library.
  JOURNAL   Malar J. 2014 Mar 12;13(1):93. doi: 10.1186/1475-2875-13-93.
  PUBMED    24620899
REFERENCE   2  (bases 1 to 9498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2014-03-12"; volume: "13(1):93. doi"; pages: " 
            10.1186/1475-2875-13-93"
COMMENT     Sequence Label: SERA4-bio-His
FEATURES             Location/Qualifiers
     source          1..9498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4662..4679
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4731..4786
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4785..4804)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7022..7040)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7108..7212
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7213..7281,7282..8073)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7431..7450)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8244..8832
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8733..8752
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8986..9003
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9120..9141
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9156..9161,9162..9179,9180..9186)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9194..9210
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9199..9221
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9218..9234
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9218..9234
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcat
     1201 ctacaacaca gggcggcgat accgataccc accctggaac acctccaggc gagggctccg
     1261 atgtgtctca gggcgctgga caggatgcta gccagggggc aggccaggac gccaatcctg
     1321 atcctaccct gcccaagccc cctagccctc ctgccgacga taccaaggat acaggcagcc
     1381 agggcgacgc cgatagcagc agctccaaga tcgagatccc cccactggtc aagcccgaga
     1441 accacaagac catcgtgtcc gccatgctga agaactacaa gggcgtgaaa gtgaccggca
     1501 cctgtggcgc cgatttcggc ctgtttctgg tgccccacat ctatgtgcac gtgaagtccg
     1561 aggacaccga gatcgagctg agcagcgaac tggcccctcc cgagatgcag accaagttcg
     1621 acaagaccca gctgaagaag ttctgcgtca aggacgacac caagaagttc gactttatcg
     1681 cctacatcta caaggacatc ctggtgttca agtggaaggt gtacgaggaa ggcctgagca
     1741 aagaacagga cgtggacgag atgaagtacc tgctgcccaa cctgaagcag cccatcacca
     1801 gcatccaggt gcacagctgg accggcacaa aagagagcta catcctggaa agcaaggact
     1861 acgtgctggg cgagggcatg cctgagaagt gcgacgctat cgccaccgac tgctttctga
     1921 gcggcttcac cgacatcggc aagtgcttcc agtgcaagct gctgatgcag gaaaagaaca
     1981 tcaacgacgc ctgcttcaaa tacgtgtcca gcaaccagaa agagctgatc aagaagcagc
     2041 tgaaaatcac cgcccaggac gacgaggaaa gcagcgagta ccacctgagc gagagcatca
     2101 agaacctgct caagaatatc tacaagaaga acaacgacga caacaagaaa aaagagctgc
     2161 tccacttcga gaacgtgaac tccgccctga agtctgagct gctgaactac tgcaacctgc
     2221 tgaaagaagt gaacatgaac ggcgtgctga aggaccacca gctgggcaac gtgcaggacg
     2281 tgttcaacaa cctggccaaa ctgctcgagg aacacaaaga agagaacgac aacgtcctgt
     2341 accacaagat gaagaacgag gccctgtgcc tgaagaatgt gaacgactgg atgaagaaca
     2401 aagccggcct gctgctgccc cagctgagct acgatctgac ctataagaac aacaacttcg
     2461 ccgagttcac ccagaacaag gcctacacca gccagaacat cgtggacaag ctgtactgca
     2521 atcacgagta ctgcaacagg ctcaaggacc acaacaactg tatcagcaag atcaacgtcg
     2581 aggaccagaa gaactgcgcc ctgtcctggg ccttcgccag caagtatcac ctggaaacca
     2641 tcaagtgcat gaagggctac gagcccctga acgccgccgt gctgtacgtg accaactgcc
     2701 tgaaaaacaa gaacaaggat gtgtgcaccg agggcagcaa ccctctggtg ttcctggaaa
     2761 caatcgagga aaagggcttc ctgcccaccg agagcaacta cccctacgac cagagcaaag
     2821 tgggcgacat ctgcccccag ctgcagaacg attgggacaa cgtgttcgag aacaccaagg
     2881 tgctggacta caacaacggc cccttcagcg tgggcaccaa gggctacatt gcctacgaga
     2941 gcgaggcctt ccagaaagac atgcacagct tcgtgaagct cgtgaaggac gagatcatga
     3001 acaagggcag cgtgatcgcc tatgtgaagg ccgagaatgt gctgggctat gagctgaacg
     3061 gcaagaaagt gcagaacctg tgcggcgata agacccccga ccacgtcgtg aatatcgtgg
     3121 gctacggcaa ctacatcaac aacaaggggg agaagaaatc ctactggatc gtgcggaact
     3181 cctggggcaa gtactggggc gacgacggct acttcaaagt ggatatgtac ggcccctcta
     3241 cctgcgagga caacttcatc cacaccgtgg tcgtgttcaa tgtgcaggtc cccatcaacg
     3301 agaagtttga caagaaagag cacgacatct ataactacta cctcaagacc agccccgagt
     3361 tctaccacaa cctgtactat aagaccttca acagcaacaa agaagaaaag tccatgaaca
     3421 agaacagcta cgtgtacggc caggacacca cccccgtgga aaatgaggcc cctagaagcg
     3481 gcgtgcagaa gcctaccgag ctgtcctcta ccgagagcca gaccgtgtcc cctccaaacg
     3541 aagcccagac agagagcctg ctgagcggcg gctcccaggt cacaaatcct gccctgaccc
     3601 agagcaccag ctccagctct ggccagcagg aaaccggccc tctgagcaca cagggactgt
     3661 ctccagccac cggcgacccc aagggcaaag agcaggaagc ttctcccgcc gagggactga
     3721 gcggagtgct gaaccctgcc aaagaagtca ccagcgaaga gaagatccag atcatccatc
     3781 tgctgaagca catcaagaat tccaagatca gacggggcct ggtcaagtac aaccacgagt
     3841 tcgaagtggg agacaacagc tgcagcagaa gcacctccaa gaacgccgaa atgcacgacg
     3901 agtgcgtgaa catctgcgag aagtactggc ccgagtgcag aggcacagcc gtgcctggct
     3961 actgtctgag cacccacgac gataagaacg agtgcgactt ctgctacgtg ggcgcgccgt
     4021 cgacctccat cacggcctat aagagtgagg gggagtcagc ggagttctcc ttcccactca
     4081 accttggaga ggaaagcctg cagggagagt tgagatggaa ggcagagaag gctccttctt
     4141 cccagtcctg gatcaccttc tccctaaaga accaaaaggt gtctgtgcag aagtctacta
     4201 gcaaccccaa gttccagctg tccgaaacgc tcccactcac ccttcagata ccccaggtct
     4261 cccttcagtt tgctggttct ggcaacctga ccctgactct ggacagaggg atactgtatc
     4321 aggaagtgaa cctggtggtg atgaaagtga ctcagcccga cagcaacact ttgacctgtg
     4381 aggtgatggg acccacctca cccaagatga gactgatctt gaagcaggag aatcaggagg
     4441 ccagggtctc caggcaggag aaagtgattc aagtgcaggc ccctgaagca ggggtgtggc
     4501 aatgtctact gagtgaaggt gaagaggtca agatggactc caagatccag gttttatcca
     4561 aagggttgaa ttccggcagc ctgcaccaca tcctggacgc ccagaagatg ctgtggaacc
     4621 acagggacag gaacctgcct cctctggccc ctctgggccc ccaccaccat caccaccatt
     4681 gagtctagac acgtgattaa ttaaggatcc cccgacctcg acctctggct aataaaggaa
     4741 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata
     4801 tgggagggca aatcatttgg tcgagatccc tcggagatct ctagctagag gatcgatccc
     4861 cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg gggcagtgca
     4921 tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca catgtgacac
     4981 ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta taaatggatg
     5041 tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag tctgtggact
     5101 gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc aatgctgggg
     5161 gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg tcaatcagag
     5221 gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata gtgtttataa
     5281 ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt atatactatc
     5341 cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc tatcgaatta
     5401 gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc tgtcacggtt
     5461 ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca caagggcagt
     5521 ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct tcttcattct
     5581 ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc
     5641 tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg gttcagtggt
     5701 ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata aatactagtg
     5761 taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg ggacaagccg
     5821 taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat aatcctagtg
     5881 caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca ggtgaaccat
     5941 gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc agcggactcc
     6001 actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg gggcccataa
     6061 acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg cgtcagcccc
     6121 cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg gctgattgta
     6181 accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg caccccgggg
     6241 aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc gatctggagg
     6301 acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc tcatattcac
     6361 gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact acccaaatat
     6421 ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat
     6481 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat
     6541 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     6601 ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc taatagagat
     6661 tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc aaatatctgg
     6721 atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     6781 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     6841 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg
     6901 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg
     6961 gtagcatatg ctatcctcat gataagctgt caaacatgag aattttcttg aagacgaaag
     7021 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     7081 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     7141 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     7201 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     7261 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     7321 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     7381 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     7441 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     7501 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     7561 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     7621 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     7681 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     7741 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     7801 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     7861 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     7921 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     7981 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     8041 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     8101 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     8161 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     8221 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     8281 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     8341 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     8401 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     8461 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     8521 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     8581 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     8641 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     8701 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     8761 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     8821 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     8881 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     8941 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     9001 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     9061 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     9121 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     9181 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     9241 tacgccaagc tctagctaga ggtcgaccaa ttctcatgtt tgacagctta tcatcgcaga
     9301 tccgggcaac gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg aagatctata
     9361 cattgaatca atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata
     9421 ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct
     9481 catgtccaat atgaccgc
//
