LOCUS       sequence-76305-e        9612 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full length SERA5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9612)
  AUTHORS   Zenonos ZA, Rayner JC, Wright GJ
  TITLE     Towards a comprehensive Plasmodium falciparum merozoite cell surface
            and secreted recombinant protein library.
  JOURNAL   Malar J. 2014 Mar 12;13(1):93. doi: 10.1186/1475-2875-13-93.
  PUBMED    24620899
REFERENCE   2  (bases 1 to 9612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2014-03-12"; volume: "13(1):93. doi"; pages: " 
            10.1186/1475-2875-13-93"
COMMENT     Sequence Label: SERA5-bio-His
FEATURES             Location/Qualifiers
     source          1..9612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4776..4793
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4845..4900
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4899..4918)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7136..7154)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7222..7326
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7327..7395,7396..8187)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7545..7564)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8358..8946
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8847..8866
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9100..9117
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9234..9255
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9270..9275,9276..9293,9294..9300)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9308..9324
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9313..9335
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9332..9348
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9332..9348
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcaa
     1201 caggcgaaag ccagacaggc aataccggcg gaggccaggc cggaaacaca ggcggagatc
     1261 aggctggctc tacaggcggc tctcctcagg gaagcacagg cgctagtcca cagggatcaa
     1321 caggcgcctc accacagggc tctaccggcg cttctcagcc tggaagcagc gagcccagca
     1381 accctgtgtc tagcggccac agcgtgtcca ccgtgtccgt gtctcagacc agcaccagca
     1441 gcgagaagca ggacaccatc caggtcaaaa gcgccctgct gaaggactac atgggcctga
     1501 aagtgaccgg cccctgcaac gagaacttca tcatgtttct ggtgccccac atctacatcg
     1561 acgtggacac cgaggacacc aacatcgagc tgcggaccac cctgaaaaag accaacaacg
     1621 ccatcagctt cgagagcaac agcggctccc tggaaaagaa gaaatacgtc aagctgccct
     1681 ccaacggcgc cacaggcgag cagggatcta gcactggaac agtgcggggc gacaccgagc
     1741 ccatcagcga tagcagcagc agctccagct cctccagtag ctcctctagc tccagttcca
     1801 gcagctctag ttctagcagt agctctagca gcgagtccct gcccgccaat ggccctgata
     1861 gccctaccgt gaagcccccc agaaacctgc agaacatctg cgaaaccggc aagaacttca
     1921 agctggtggt gtacatcaaa gagaacaccc tgatcctgaa gtggaaggtg tacggcgaga
     1981 caaaggacac caccgagaac aacaaggtgg acgtgcggaa gtacctgatc aacgagaaag
     2041 aaaccccctt caccaatatc ctgatccacg cctacaaaga gcacaacggg gccaacctga
     2101 tcgagtccaa gaactacgcc atcggcagcg acatccccga gaagtgcgat accctggcca
     2161 gcaactgctt cctgagcggc aacttcaaca ttgagaagtg cttccagtgt gccctgctgg
     2221 tggaaaaaga aaacaagaac gacgtgtgct acaagtacct gtccgaggac atcgtgtcca
     2281 agttcaaaga gatcaaggcc gagacagagg acgacgacga ggacgactac accgagtaca
     2341 agctgaccga gagcatcgac aacatcctgg tcaagatgtt caagaccaat gagaacaacg
     2401 acaagagcga gctgatcaag ctggaagagg tggacgacag cctgaaactg gaactgatga
     2461 actactgcag cctgctcaag gatgtggata ccaccggcac cctggacaac tacggcatgg
     2521 gcaacgagat ggacatcttc aacaacctga agcggctgct gatctaccac agcgaggaaa
     2581 acatcaacac cctcaagaac aagttccgca acgccgccgt gtgcctgaag aacgtggacg
     2641 actggatcgt gaacaagcgg ggcctggtgc tgcccgagct gaactacgac ctggaatact
     2701 tcaacgagca cctgtacaac gataagaaca gccccgagga caaggacaac aagggcaagg
     2761 gcgtggtgca tgtggacacc acactcgaga aagaggatac cctgagctac gacaacagcg
     2821 acaacatgtt ctgtaacaaa gagtactgca accgcctgaa ggacgagaac aattgcatca
     2881 gcaatctgca ggtcgaggac cagggcaact gcgacaccag ctggatcttc gccagcaagt
     2941 accacctgga aaccatccgg tgcatgaagg gctacgagcc caccaagatc agcgccctgt
     3001 acgtggccaa ctgctacaag ggcgagcaca aggatagatg cgacgagggc agcagcccta
     3061 tggaattcct gcagattatc gaggactacg gcttcctgcc tgccgagtcc aactacccct
     3121 acaactacgt gaaagtgggc gagcagtgcc ccaaggtgga agatcactgg atgaacctgt
     3181 gggacaacgg caagatcctg cacaacaaga atgagcccaa cagcctggac ggcaagggct
     3241 acacagccta cgagagcgag cggttccacg acaatatgga cgccttcgtg aagatcatca
     3301 agaccgaagt catgaacaag ggctccgtga tcgcctacat caaggcagag aacgtgatgg
     3361 gctatgagtt cagcggcaag aaagtgcaga acctgtgcgg cgacgacacc gccgatcacg
     3421 ccgtgaatat cgtgggctac ggcaattacg tgaacagcga gggcgagaag aaaagctatt
     3481 ggattgtgcg gaacagctgg ggcccctact ggggcgacga aggctacttc aaagtggata
     3541 tgtacggccc cacccactgc cacttcaact tcatccacag cgtcgtgatc ttcaacgtgg
     3601 acctgcccat gaacaacaaa gccaccaaga aagagagcaa gatctacgac tactacctga
     3661 aggcctcccc cgagttctac cacaacctgt acttcaagaa ctttaacgtg ggcaagaaga
     3721 acctgttcag cgaaaaagag gacaacgaaa acaacaaaaa gctgggcaac aactacatca
     3781 tcttcggcca ggataccgcc ggctccggcc agtctggcaa agagtctaac accgccctgg
     3841 aaagcgccgg cacctccaat gaggtgtccg agagggtgca cgtgtaccac attctgaagc
     3901 acatcaagga cgggaagatc cggatgggca tgagaaagta catcgacacc caggacgtca
     3961 acaagaagca cagctgcacc agaagctacg ccttcaaccc cgagaactac gagaaatgcg
     4021 tcaacctgtg caacgtgaac tggaaaacct gcgaagagaa aaccagccct ggcctgtgcc
     4081 tgtccaagct ggacacaaac aacgagtgct acttctgcta cgtgggcgcg ccgtcgacct
     4141 ccatcacggc ctataagagt gagggggagt cagcggagtt ctccttccca ctcaaccttg
     4201 gagaggaaag cctgcaggga gagttgagat ggaaggcaga gaaggctcct tcttcccagt
     4261 cctggatcac cttctcccta aagaaccaaa aggtgtctgt gcagaagtct actagcaacc
     4321 ccaagttcca gctgtccgaa acgctcccac tcacccttca gataccccag gtctcccttc
     4381 agtttgctgg ttctggcaac ctgaccctga ctctggacag agggatactg tatcaggaag
     4441 tgaacctggt ggtgatgaaa gtgactcagc ccgacagcaa cactttgacc tgtgaggtga
     4501 tgggacccac ctcacccaag atgagactga tcttgaagca ggagaatcag gaggccaggg
     4561 tctccaggca ggagaaagtg attcaagtgc aggcccctga agcaggggtg tggcaatgtc
     4621 tactgagtga aggtgaagag gtcaagatgg actccaagat ccaggtttta tccaaagggt
     4681 tgaattccgg cagcctgcac cacatcctgg acgcccagaa gatgctgtgg aaccacaggg
     4741 acaggaacct gcctcctctg gcccctctgg gcccccacca ccatcaccac cattgagtct
     4801 agacacgtga ttaattaagg atcccccgac ctcgacctct ggctaataaa ggaaatttat
     4861 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     4921 ggcaaatcat ttggtcgaga tccctcggag atctctagct agaggatcga tccccgcccc
     4981 ggacgaacta aacctgacta cgacatctct gccccttctt cgcggggcag tgcatgtaat
     5041 cccttcagtt ggttggtaca acttgccaac tgggccctgt tccacatgtg acacgggggg
     5101 ggaccaaaca caaaggggtt ctctgactgt agttgacatc cttataaatg gatgtgcaca
     5161 tttgccaaca ctgagtggct ttcatcctgg agcagacttt gcagtctgtg gactgcaaca
     5221 caacattgcc tttatgtgta actcttggct gaagctctta caccaatgct gggggacatg
     5281 tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc agaggggcct
     5341 gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt ataaggcccc
     5401 cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac tatccagact
     5461 aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga attagggtta
     5521 gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac ggttttattt
     5581 acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg cagtggctga
     5641 agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca ttctccttcg
     5701 tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag gtgctcgaaa
     5761 acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag tggtggcatt
     5821 gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact agtgtaggaa
     5881 tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa gccgtaaaga
     5941 ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct agtgcaatat
     6001 gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa ccatgttgtt
     6061 acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga ctccactggt
     6121 tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc ataaacaaag
     6181 acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag cccccacacg
     6241 ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat tgtaaccccg
     6301 ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc ggggaatacc
     6361 tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg gaggacaaat
     6421 tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat tcacgaggtc
     6481 gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa atatctggat
     6541 agcatatgct atcctaatct atatctgggt agcataggct atcctaatct atatctgggt
     6601 agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt
     6661 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     6721 agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag agattagggt
     6781 agtatatgct atcctaattt atatctgggt agcatatact acccaaatat ctggatagca
     6841 tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagca
     6901 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     6961 tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca
     7021 tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca
     7081 tatgctatcc tcatgataag ctgtcaaaca tgagaatttt cttgaagacg aaagggcctc
     7141 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     7201 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     7261 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     7321 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     7381 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     7441 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     7501 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     7561 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     7621 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     7681 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     7741 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     7801 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     7861 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     7921 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     7981 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     8041 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     8101 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     8161 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     8221 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     8281 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     8341 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     8401 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     8461 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     8521 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     8581 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     8641 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     8701 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     8761 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     8821 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     8881 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     8941 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     9001 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     9061 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     9121 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     9181 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     9241 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     9301 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     9361 aagctctagc tagaggtcga ccaattctca tgtttgacag cttatcatcg cagatccggg
     9421 caacgttgtt gccattgctg caggcgcaga actggtaggt atggaagatc tatacattga
     9481 atcaatattg gcaattagcc atattagtca ttggttatat agcataaatc aatattggct
     9541 attggccatt gcatacgttg tatctatatc ataatatgta catttatatt ggctcatgtc
     9601 caatatgacc gc
//
