LOCUS       sequence-76308-e        9417 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full length SERA9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9417)
  AUTHORS   Zenonos ZA, Rayner JC, Wright GJ
  TITLE     Towards a comprehensive Plasmodium falciparum merozoite cell surface
            and secreted recombinant protein library.
  JOURNAL   Malar J. 2014 Mar 12;13(1):93. doi: 10.1186/1475-2875-13-93.
  PUBMED    24620899
REFERENCE   2  (bases 1 to 9417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2014-03-12"; volume: "13(1):93. doi"; pages: " 
            10.1186/1475-2875-13-93"
COMMENT     Sequence Label: SERA9-bio-His
FEATURES             Location/Qualifiers
     source          1..9417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4581..4598
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4650..4705
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4704..4723)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6941..6959)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7027..7131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7132..7200,7201..7992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7350..7369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8163..8751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8652..8671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8905..8922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9039..9060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9075..9080,9081..9098,9099..9105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9113..9129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9118..9140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9137..9153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9137..9153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcag
     1201 aggtggaaag cagcgacagc accagctttc tgaacctgat cagcggccag agcagcagca
     1261 acagcgacga caccgtgtcc agcagcgact ccagaggcgt ggtggcctct gtgtctggca
     1321 ccgtggatag cgacgtgtcc cggaccgata gcaccggcaa cgtgaacagc aagaacagcg
     1381 agctgagcga gacacggcag aacagcgtgg aacagaacaa caacgccaac tccgccgaca
     1441 acagcatcaa gatcaagagc gccttcctga aagaaaacgt gggcgtgaag atcaccggcc
     1501 cctgcgacga gggattcggc ctggtcatgg tgccccacat catcatcaac gtggacaccc
     1561 ggaacaccga catccagctg ggcaagaagt ccagcgagat caactacggc atcaagctgc
     1621 ggaagaaggg caaggacgag tacgaggaaa aagtgggcat cgacctgctg aagaacgtgt
     1681 gcgaaaccgg caagaacttc aagttcgtgg cctacatcaa gggcgacgag ctgatcatta
     1741 agtggaaagt gatcgaggaa ggcaacatcc ccatcgacat caagaagttt aagatcaagc
     1801 ggatgagccc ccccatcacc agcatccagg tgttcagcag catgtccgac gagaagtcct
     1861 tcgtgctgga aaacaagaac tacgtggtca ccaacaacat gcccgagaag tgcgaggccc
     1921 tggccaccaa ctgttttctg agcggcaata tcgacattga gaagtgctac cagtgcagcc
     1981 tgctggtgga aaacgacgcc aagtccgacg tgtgctacaa cttcatcagc cgcgagaaca
     2041 aagagaagct gaacgaggaa cggttcaaag tgaagggctc caccgagtac gaagagagcg
     2101 tggaatccga gctgaagggc gccattgatc tgctgatctc cagcttctac aagagcgacg
     2161 gcgagaatgg cgagaacgtg ctgattagct tcgaggacct gaacagcgcc ctcgaggacg
     2221 acatcatcaa tttctgctcc ctgctgaaac tggtggacct gtccggcacc ttcatgaacc
     2281 accagatcgg caccaccgcc gacgccttca acaacatcgt gaagctgctg aaagagcaca
     2341 aagaagagga cgagttcgtc ctgcggacca agctgagaaa ccccgccctg tgcatgaaga
     2401 atgtggacga ctgggtcaag agcagatgcg gcctgctgac ctccgacgac agctacgtcg
     2461 tgatcaagaa gaactccgac accaacggga agaacgagaa caacgaggcc aatgacgagg
     2521 aatacgacgg cgtgatcgac ctgagcacca acgagaacgg caatgtgaag tccatccagt
     2581 tcaacgacga catctactgc aacgacgatt actgcaatcg gtggaaaaat gagaacaact
     2641 gcatctccaa gatcggcgtc gaggaccagg gcaactgtgc cagctcttgg gccttcgcct
     2701 ccaagctgca cttcgagaca ctgagatgca tgaagggcta cgaccacctg ggcatcagcg
     2761 ccctgtacct gaccaattgc gccaagggca agatcagcaa gagatgcacc gagggcggca
     2821 accccgccga gttcctggaa atcgtgtcca actccggctt cctgcccacc gagttcgact
     2881 acccctactt ctacgccctg gtcgagaaca gctgccccag cggcctgaac aagtggatca
     2941 acctgtggga ggatgtgaag ctgttcgact ataagtacga gcccaactcc atcggcagca
     3001 agggctacag cgtgtacaag tccgagatgt tcaaggacaa catggatgag tacgtgaaga
     3061 tgatcaagtc tgagatcaag aacaagggca gcgtgatcgc ctatatcaac gcccagagcg
     3121 tgctgaccta cgatttcaac ggcaagaata tcctgaacat gtgcggctcc aagaccaccg
     3181 accactccat caatgtgatc ggctacggcc actacaaaaa caaagagggc gaggaaaagt
     3241 cctactggct cgtgcggaac agctggggct actactgggg cgacgaaggc aacttcaaag
     3301 tggatatgta cggccccagc aactgcaact accactttat caacaccgtg ctggtgttca
     3361 acatcgatat ccccatgatg aacaacaacg aaaacaacaa gaagggcctg tacaactact
     3421 tccccaagta cacccacgag ttctaccaca acttctacga ccagaacttc ggcagcgaca
     3481 acaagatctc cctgatcaac agcaacggcg ccaacaagaa cgatgtgaac aacgcctaca
     3541 cagtgcacgg ccagtccggc gagacagagg tgcagcctag ccagctgaga agcaccgcca
     3601 accaggacag caacagaggc gccgtgacca gcgaccctct gagaacccag cctgagacaa
     3661 ccagcacctc cgtgaagaaa atggacgtgt tccaggtgct gaagcacgtc aagaatagca
     3721 agatcaaagt gggcctgatt aagtacgaca acgatgacga gatcggcgcc caccacagct
     3781 gcaccagagc ctacagcttc gaccctgaga agtacgacga gtgtgtggaa ttctgccgga
     3841 agaactggga gcagtgcaag gacaagccca gccccggctt ctgcctgaca aagctggatg
     3901 agtccaaggg ctgcttcttc tgctacgtgg gcgcgccgtc gacctccatc acggcctata
     3961 agagtgaggg ggagtcagcg gagttctcct tcccactcaa ccttggagag gaaagcctgc
     4021 agggagagtt gagatggaag gcagagaagg ctccttcttc ccagtcctgg atcaccttct
     4081 ccctaaagaa ccaaaaggtg tctgtgcaga agtctactag caaccccaag ttccagctgt
     4141 ccgaaacgct cccactcacc cttcagatac cccaggtctc ccttcagttt gctggttctg
     4201 gcaacctgac cctgactctg gacagaggga tactgtatca ggaagtgaac ctggtggtga
     4261 tgaaagtgac tcagcccgac agcaacactt tgacctgtga ggtgatggga cccacctcac
     4321 ccaagatgag actgatcttg aagcaggaga atcaggaggc cagggtctcc aggcaggaga
     4381 aagtgattca agtgcaggcc cctgaagcag gggtgtggca atgtctactg agtgaaggtg
     4441 aagaggtcaa gatggactcc aagatccagg ttttatccaa agggttgaat tccggcagcc
     4501 tgcaccacat cctggacgcc cagaagatgc tgtggaacca cagggacagg aacctgcctc
     4561 ctctggcccc tctgggcccc caccaccatc accaccattg agtctagaca cgtgattaat
     4621 taaggatccc ccgacctcga cctctggcta ataaaggaaa tttattttca ttgcaatagt
     4681 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttggt
     4741 cgagatccct cggagatctc tagctagagg atcgatcccc gccccggacg aactaaacct
     4801 gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt cagttggttg
     4861 gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc aaacacaaag
     4921 gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc caacactgag
     4981 tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca ttgcctttat
     5041 gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct cccaggggcc
     5101 caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta gctaccgata
     5161 agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt taaccctaaa
     5221 cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc taattcaata
     5281 gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa agggtcctaa
     5341 ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg gggtcaggat
     5401 tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc aaggagcggg
     5461 cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag ctaatagaat
     5521 aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag gtttcaggtg
     5581 acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct atgacaccaa
     5641 tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa cattctgaat
     5701 atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga tgtccatctc
     5761 acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac tggggttatt
     5821 aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact ctatttgtaa
     5881 caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct ctaacacccc
     5941 cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag tggccactct
     6001 tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc ctgcggtttt
     6061 ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac cactgcggtc
     6121 aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat aagtaggtgg
     6181 gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac acacttgcgc
     6241 ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga gagcacggtg
     6301 ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat atgctatcct
     6361 aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     6421 aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct
     6481 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     6541 aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat atgctatcct
     6601 aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc
     6661 tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc
     6721 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt
     6781 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     6841 tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctcatg
     6901 ataagctgtc aaacatgaga attttcttga agacgaaagg gcctcgtgat acgcctattt
     6961 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     7021 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     7081 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     7141 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     7201 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     7261 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     7321 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     7381 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     7441 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     7501 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     7561 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     7621 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     7681 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     7741 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     7801 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     7861 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     7921 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     7981 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     8041 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     8101 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     8161 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     8221 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     8281 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     8341 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     8401 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     8461 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     8521 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     8581 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     8641 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     8701 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     8761 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     8821 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     8881 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     8941 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     9001 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     9061 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     9121 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct ctagctagag
     9181 gtcgaccaat tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat
     9241 tgctgcaggc gcagaactgg taggtatgga agatctatac attgaatcaa tattggcaat
     9301 tagccatatt agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata
     9361 cgttgtatct atatcataat atgtacattt atattggctc atgtccaata tgaccgc
//
