LOCUS       sequence-76309-e        8016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full length PFA0210c.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8016)
  AUTHORS   Zenonos ZA, Rayner JC, Wright GJ
  TITLE     Towards a comprehensive Plasmodium falciparum merozoite cell surface
            and secreted recombinant protein library.
  JOURNAL   Malar J. 2014 Mar 12;13(1):93. doi: 10.1186/1475-2875-13-93.
  PUBMED    24620899
REFERENCE   2  (bases 1 to 8016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2014-03-12"; volume: "13(1):93. doi"; pages: " 
            10.1186/1475-2875-13-93"
COMMENT     Sequence Label: PFA0210c-bio-His
FEATURES             Location/Qualifiers
     source          1..8016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3180..3197
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3249..3304
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3303..3322)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5540..5558)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5626..5730
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5731..5799,5800..6591)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5949..5968)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6762..7350
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7251..7270
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7504..7521
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7638..7659
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7674..7679,7680..7697,7698..7704)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7712..7728
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7717..7739
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7736..7752
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7736..7752
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcat
     1201 acgacaacat catcaacaac tacggcggcc tgagcagcgt ggtggtgtac aacaagaacg
     1261 tgggcatcaa caagatcggc gacaacgtgt acaagaacaa gatcaagagc cggatcctga
     1321 aagagaacaa agaggaatcc ctggaaacag ccgccgtgaa cgagaacacc aaggacgtga
     1381 aggacagctg caactacgcc acccccagca gcgtgttcaa caacgacaaa gagaataccg
     1441 ccagcttcat caagaccggc gagagctacg acggcaacaa cgtgcaggct caggcccagg
     1501 cacaggctca ggcagaagcc gagggcagcc tggaaaacgc tgctgtgaac ctgcggacca
     1561 agagcgccac cctgatcaac gacggcatgc tggactacta cctgaacttc tgcgatcagg
     1621 tcaacaacaa ggccttccca ttcaacgagt gcaccaacct gcacaccagc gacatggtca
     1681 ccctgtgcaa gaacaacaac gatgagaagt ccaagatcac ctacgacctg ctgggctacg
     1741 gcgagatgga cgacgtgtcc gcctacagcc tgagctacgc cctgaacgac gtggaaacca
     1801 tcaaggactg gaacaagaac atctacaagc tgaactacct caacctgaag aagtccgaca
     1861 tccaggaaaa gatcgacaag aacgagaaga tcaacgtgaa caacttcatc ctgcagagcg
     1921 agaaggacgt gcccgaggac gagcggaagt acctgtacct gatcaatggc ctgccctggc
     1981 ccttcaagag ccaggacacc atctacgagg tgtaccagaa gtactacaac aacaaaaaca
     2041 tgctgctgat cattaacaag gccctcaacg atgtgttcga caacaacgcc gcctacgccc
     2101 ggatcaacaa ttacgagaac ttcttctgca tctaccccaa gagcaagaac agctacgaca
     2161 agggcgtgaa atacgtgatg agcatcatct acgacgtgaa catccccaag ttcatccaga
     2221 acaacatcct gaaccagatc ttccccgacc tgatcttcaa tctgcacaac accgccattg
     2281 ccatcacaaa caaggccgtg ggcaccgtgg tggacctgag caagaatgag cagaacgcct
     2341 ggcacgccca ctccctgaag aacgtgaagc ccgaggatac ccccaacacc gagcacgtgc
     2401 agaccgaccc cgagaactct tacggcctgg gctttatcaa gatgatcttc gtggacggcc
     2461 cctacaacct gtggattatc aacgtcaact tcttcaagaa gatctttggc atcttcttca
     2521 acaaggatgg cgcgccgtcg acctccatca cggcctataa gagtgagggg gagtcagcgg
     2581 agttctcctt cccactcaac cttggagagg aaagcctgca gggagagttg agatggaagg
     2641 cagagaaggc tccttcttcc cagtcctgga tcaccttctc cctaaagaac caaaaggtgt
     2701 ctgtgcagaa gtctactagc aaccccaagt tccagctgtc cgaaacgctc ccactcaccc
     2761 ttcagatacc ccaggtctcc cttcagtttg ctggttctgg caacctgacc ctgactctgg
     2821 acagagggat actgtatcag gaagtgaacc tggtggtgat gaaagtgact cagcccgaca
     2881 gcaacacttt gacctgtgag gtgatgggac ccacctcacc caagatgaga ctgatcttga
     2941 agcaggagaa tcaggaggcc agggtctcca ggcaggagaa agtgattcaa gtgcaggccc
     3001 ctgaagcagg ggtgtggcaa tgtctactga gtgaaggtga agaggtcaag atggactcca
     3061 agatccaggt tttatccaaa gggttgaatt ccggcagcct gcaccacatc ctggacgccc
     3121 agaagatgct gtggaaccac agggacagga acctgcctcc tctggcccct ctgggccccc
     3181 accaccatca ccaccattga gtctagacac gtgattaatt aaggatcccc cgacctcgac
     3241 ctctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     3301 ctcactcgga aggacatatg ggagggcaaa tcatttggtc gagatccctc ggagatctct
     3361 agctagagga tcgatccccg ccccggacga actaaacctg actacgacat ctctgcccct
     3421 tcttcgcggg gcagtgcatg taatcccttc agttggttgg tacaacttgc caactgggcc
     3481 ctgttccaca tgtgacacgg ggggggacca aacacaaagg ggttctctga ctgtagttga
     3541 catccttata aatggatgtg cacatttgcc aacactgagt ggctttcatc ctggagcaga
     3601 ctttgcagtc tgtggactgc aacacaacat tgcctttatg tgtaactctt ggctgaagct
     3661 cttacaccaa tgctggggga catgtacctc ccaggggccc aggaagacta cgggaggcta
     3721 caccaacgtc aatcagaggg gcctgtgtag ctaccgataa gcggaccctc aagagggcat
     3781 tagcaatagt gtttataagg cccccttgtt aaccctaaac gggtagcata tgcttcccgg
     3841 gtagtagtat atactatcca gactaaccct aattcaatag catatgttac ccaacgggaa
     3901 gcatatgcta tcgaattagg gttagtaaaa gggtcctaag gaacagcgat atctcccacc
     3961 ccatgagctg tcacggtttt atttacatgg ggtcaggatt ccacgagggt agtgaaccat
     4021 tttagtcaca agggcagtgg ctgaagatca aggagcgggc agtgaactct cctgaatctt
     4081 cgcctgcttc ttcattctcc ttcgtttagc taatagaata actgctgagt tgtgaacagt
     4141 aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga ataaaatttg
     4201 gacggggggt tcagtggtgg cattgtgcta tgacaccaat ataaccctca caaacccctt
     4261 gggcaataaa tactagtgta ggaatgaaac attctgaata tctttaacaa tagaaatcca
     4321 tggggtgggg acaagccgta aagactggat gtccatctca cacgaattta tggctatggg
     4381 caacacataa tcctagtgca atatgatact ggggttatta agatgtgtcc caggcaggga
     4441 ccaagacagg tgaaccatgt tgttacactc tatttgtaac aaggggaaag agagtggacg
     4501 ccgacagcag cggactccac tggttgtctc taacaccccc gaaaattaaa cggggctcca
     4561 cgccaatggg gcccataaac aaagacaagt ggccactctt ttttttgaaa ttgtggagtg
     4621 ggggcacgcg tcagccccca cacgccgccc tgcggttttg gactgtaaaa taagggtgta
     4681 ataacttggc tgattgtaac cccgctaacc actgcggtca aaccacttgc ccacaaaacc
     4741 actaatggca ccccggggaa tacctgcata agtaggtggg cgggccaaga taggggcgcg
     4801 attgctgcga tctggaggac aaattacaca cacttgcgcc tgagcgccaa gcacagggtt
     4861 gttggtcctc atattcacga ggtcgctgag agcacggtgg gctaatgttg ccatgggtag
     4921 catatactac ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata
     4981 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     5041 tgctatccta atttatatct gggtagcata ggctatccta atctatatct gggtagcata
     5101 tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata
     5161 tgctatccta atagagatta gggtagtata tgctatccta atttatatct gggtagcata
     5221 tactacccaa atatctggat agcatatgct atcctaatct atatctgggt agcatatgct
     5281 atcctaatct atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     5341 atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt agcataggct
     5401 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     5461 atcctaatct gtatccgggt agcatatgct atcctcatga taagctgtca aacatgagaa
     5521 ttttcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     5581 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5641 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5701 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5761 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5821 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5881 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5941 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     6001 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     6061 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     6121 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     6181 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6241 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
     6301 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6361 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6421 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     6481 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6541 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6601 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6661 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6721 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6781 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6841 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6901 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6961 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7021 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7081 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7141 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7201 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7261 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7321 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7381 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     7441 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     7501 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     7561 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     7621 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     7681 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     7741 aacagctatg accatgatta cgccaagctc tagctagagg tcgaccaatt ctcatgtttg
     7801 acagcttatc atcgcagatc cgggcaacgt tgttgccatt gctgcaggcg cagaactggt
     7861 aggtatggaa gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
     7921 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
     7981 tgtacattta tattggctca tgtccaatat gaccgc
//
