LOCUS       sequence-7633-ex        9063 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9063)
  TITLE     Soriano vectors
REFERENCE   2  (bases 1 to 9063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SABgeolox2DTA
FEATURES             Location/Qualifiers
     source          1..9063
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     677..693
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    707..740
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    join(805..870,871..908)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     CDS             959..4000
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQ"
     CDS             4025..4828
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4088..4107)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4698..4717
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4866..5090
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    5163..5196
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        5275..5774
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5310..5333)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5707..5726
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5801..6373
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          6450..6515
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    6589..6813
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6875..6895)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6875..6893)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6914..6930)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6914..6930)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6927..6949)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6938..6954
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6962..6968,6969..6986,6987..6992))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7007..7028
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7145..7162)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7316..7904)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7396..7415)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8075..8866,8867..8935))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8698..8717
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8936..9040)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgctcta gaactagtgg atccggaacc cttaatataa cttcgtataa
      721 tgtatgctat acgaagttat taggtccctc gacctgcagg aatttcgagg ctagaactag
      781 tggatccccc gggctgcaga tctgtagggc gcagtagtcc agggtttcct tgatgatgtc
      841 atacttatcc tgtccctttt ttttccacag ctcgcggttg aggacaaact cttcgcggtc
      901 tttccagtgg ggatcgacgg tatcgataag cttgatgatc tgtgacatgg cggatcccgt
      961 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     1021 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     1081 acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt
     1141 gccggaaagc tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa
     1201 ctggcagatg cacggttacg atgcgcccat ctacaccaac gtaacctatc ccattacggt
     1261 caatccgccg tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt
     1321 tgatgaaagc tggctacagg aaggccagac gcgaattatt tttgatggcg ttaactcggc
     1381 gtttcatctg tggtgcaacg ggcgctgggt cggttacggc caggacagtc gtttgccgtc
     1441 tgaatttgac ctgagcgcat ttttacgcgc cggagaaaac cgcctcgcgg tgatggtgct
     1501 gcgttggagt gacggcagtt atctggaaga tcaggatatg tggcggatga gcggcatttt
     1561 ccgtgacgtc tcgttgctgc ataaaccgac tacacaaatc agcgatttcc atgttgccac
     1621 tcgctttaat gatgatttca gccgcgctgt actggaggct gaagttcaga tgtgcggcga
     1681 gttgcgtgac tacctacggg taacagtttc tttatggcag ggtgaaacgc aggtcgccag
     1741 cggcaccgcg cctttcggcg gtgaaattat cgatgagcgt ggtggttatg ccgatcgcgt
     1801 cacactacgt ctgaacgtcg aaaacccgaa actgtggagc gccgaaatcc cgaatctcta
     1861 tcgtgcggtg gttgaactgc acaccgccga cggcacgctg attgaagcag aagcctgcga
     1921 tgtcggtttc cgcgaggtgc ggattgaaaa tggtctgctg ctgctgaacg gcaagccgtt
     1981 gctgattcga ggcgttaacc gtcacgagca tcatcctctg catggtcagg tcatggatga
     2041 gcagacgatg gtgcaggata tcctgctgat gaagcagaac aactttaacg ccgtgcgctg
     2101 ttcgcattat ccgaaccatc cgctgtggta cacgctgtgc gaccgctacg gcctgtatgt
     2161 ggtggatgaa gccaatattg aaacccacgg catggtgcca atgaatcgtc tgaccgatga
     2221 tccgcgctgg ctaccggcga tgagcgaacg cgtaacgcga atggtgcagc gcgatcgtaa
     2281 tcacccgagt gtgatcatct ggtcgctggg gaatgaatca ggccacggcg ctaatcacga
     2341 cgcgctgtat cgctggatca aatctgtcga tccttcccgc ccggtgcagt atgaaggcgg
     2401 cggagccgac accacggcca ccgatattat ttgcccgatg tacgcgcgcg tggatgaaga
     2461 ccagcccttc ccggctgtgc cgaaatggtc catcaaaaaa tggctttcgc tacctggaga
     2521 gacgcgcccg ctgatccttt gcgaatacgc ccacgcgatg ggtaacagtc ttggcggttt
     2581 cgctaaatac tggcaggcgt ttcgtcagta tccccgttta cagggcggct tcgtctggga
     2641 ctgggtggat cagtcgctga ttaaatatga tgaaaacggc aacccgtggt cggcttacgg
     2701 cggtgatttt ggcgatacgc cgaacgatcg ccagttctgt atgaacggtc tggtctttgc
     2761 cgaccgcacg ccgcatccag cgctgacgga agcaaaacac cagcagcagt ttttccagtt
     2821 ccgtttatcc gggcaaacca tcgaagtgac cagcgaatac ctgttccgtc atagcgataa
     2881 cgagctcctg cactggatgg tggcgctgga tggtaagccg ctggcaagcg gtgaagtgcc
     2941 tctggatgtc gctccacaag gtaaacagtt gattgaactg cctgaactac cgcagccgga
     3001 gagcgccggg caactctggc tcacagtacg cgtagtgcaa ccgaacgcga ccgcatggtc
     3061 agaagccggg cacatcagcg cctggcagca gtggcgtctg gcggaaaacc tcagtgtgac
     3121 gctccccgcc gcgtcccacg ccatcccgca tctgaccacc agcgaaatgg atttttgcat
     3181 cgagctgggt aataagcgtt ggcaatttaa ccgccagtca ggctttcttt cacagatgtg
     3241 gattggcgat aaaaaacaac tgctgacgcc gctgcgcgat cagttcaccc gtgcaccgct
     3301 ggataacgac attggcgtaa gtgaagcgac ccgcattgac cctaacgcct gggtcgaacg
     3361 ctggaaggcg gcgggccatt accaggccga agcagcgttg ttgcagtgca cggcagatac
     3421 acttgctgat gcggtgctga ttacgaccgc tcacgcgtgg cagcatcagg ggaaaacctt
     3481 atttatcagc cggaaaacct accggattga tggtagtggt caaatggcga ttaccgttga
     3541 tgttgaagtg gcgagcgata caccgcatcc ggcgcggatt ggcctgaact gccagctggc
     3601 gcaggtagca gagcgggtaa actggctcgg attagggccg caagaaaact atcccgaccg
     3661 ccttactgcc gcctgttttg accgctggga tctgccattg tcagacatgt ataccccgta
     3721 cgtcttcccg agcgaaaacg gtctgcgctg cgggacgcgc gaattgaatt atggcccaca
     3781 ccagtggcgc ggcgacttcc agttcaacat cagccgctac agtcaacagc aactgatgga
     3841 aaccagccat cgccatctgc tgcacgcgga agaaggcaca tggctgaata tcgacggttt
     3901 ccatatgggg attggtggcg acgactcctg gagcccgtca gtatcggcgg aattccagct
     3961 gagcgccggt cgctaccatt accagttggt ctggtgtcag gggatccccc gggctgcagc
     4021 caatatggga tcggccattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     4081 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     4141 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     4201 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     4261 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     4321 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     4381 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     4441 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     4501 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     4561 gcgcatgccc gacggcgatg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     4621 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     4681 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     4741 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     4801 ctatcgcctt cttgacgagt tcttctgagg ggatcaattc tctagagctc gctgatcagc
     4861 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     4921 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     4981 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     5041 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgagac
     5101 ggaaagaacc agctggggct cgatcctcta gagtcgaatc aagctgatcc ggaaccctta
     5161 atataacttc gtataatgta tgctatacga agttattagg tccctcgacc tgcagcccaa
     5221 gctagcttat cgataccgtc gacggtatcg ataagcttga tatcgaattc taccgggtag
     5281 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
     5341 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
     5401 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
     5461 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
     5521 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
     5581 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
     5641 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
     5701 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
     5761 ctccgggcct ttcgacctgc aggtcctcgc catggatcct gatgatgttg ttgattcttc
     5821 taaatctttt gtgatggaaa acttttcttc gtaccacggg actaaacctg gttatgtaga
     5881 ttccattcaa aaaggtatac aaaagccaaa atctggtaca caaggaaatt atgacgatga
     5941 ttggaaaggg ttttatagta ccgacaataa atacgacgct gcgggatact ctgtagataa
     6001 tgaaaacccg ctctctggaa aagctggagg cgtggtcaaa gtgacgtatc caggactgac
     6061 gaaggttctc gcactaaaag tggataatgc cgaaactatt aagaaagagt taggtttaag
     6121 tctcactgaa ccgttgatgg agcaagtcgg aacggaagag tttatcaaaa ggttcggtga
     6181 tggtgcttcg cgtgtagtgc tcagccttcc cttcgctgag gggagttcta gcgttgaata
     6241 tattaataac tgggaacagg cgaaagcgtt aagcgtagaa cttgagatta attttgaaac
     6301 ccgtggaaaa cgtggccaag atgcgatgta tgagtatatg gctcaagcct gtgcaggaaa
     6361 tcgtgtcagg cgatctcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca
     6421 aactacctac agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg
     6481 ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat
     6541 gggagcagtg gtggaatgca gatcctagag ctcgctgatc agcctcgact gtgccttcta
     6601 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     6661 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     6721 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     6781 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     6841 gctcgagggg gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct
     6901 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     6961 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7021 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7081 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7141 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7201 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7261 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7321 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7381 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7441 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7501 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7561 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7621 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7681 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     7741 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7801 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7861 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7921 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7981 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8041 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8101 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8161 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8221 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8281 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8341 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     8401 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8461 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8521 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8581 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     8641 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     8701 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     8761 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8821 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8881 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     8941 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9001 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9061 cac
//
