LOCUS       sequence-76387-e        9171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human NKCC1 with truncation of N-terminus and mutations at
            P676C I730C. Contains an N-terminal 3xFLAG-YFP tag for expression in
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9171)
  AUTHORS   Monette MY, Somasekharan S, Forbush B
  TITLE     Molecular Motions Involved in Na-K-Cl Cotransporter-mediated Ion 
            Transport and Transporter Activation Revealed by Internal 
            Crosslinking between Transmembrane Domains 10 and 11/12.
  JOURNAL   J Biol Chem. 2014 Jan 22.
  PUBMED    24451383
REFERENCE   2  (bases 1 to 9171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 Jan 22."
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-termina...
FEATURES             Location/Qualifiers
     source          1..9171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             31..96
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             join(109..111,112..822)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(109..111,112..822)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(151..172)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(412..431)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     759..780
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3871..3888)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3877..4101
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4147..4575
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4234..4253)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4444..4465
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4584..4604)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4589..4918
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4769..4904
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4831..4850
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4985..5779
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5039..5058)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5649..5668
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5953..6074
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5990..6009)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6044..6063
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6123..6139)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6123..6139)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6136..6158)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6147..6163
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6171..6177,6178..6195,6196..6201))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6216..6237
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6354..6371)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6525..7110)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6605..6624)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7281..8072,8073..8141))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7904..7923
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8142..8246)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8321..8340)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8512..8891
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8892..9095
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9046..9066
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9140..9159
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9140..9158
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctagcccgg gcacggccgc caccatggga gactacaaag accatgacgg tgattataaa
       61 gatcacgaca tcgactacaa ggatgacgat gacaagggtg accctaccgt cagcaagggc
      121 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      181 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      241 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc
      301 ggctacggcc tgatgtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
      361 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      421 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      481 ctgaagggca tcgacttcaa ggaggacggc aacatcctag ggcacaagct ggagtacaac
      541 tacaacagcc acaacgttta tatcatggcc gacaagcaga agaacggcat caaggtgaac
      601 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
      661 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
      721 tccaaactga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
      781 accgccgccg ggatcactct cggcatggac gagctctaca agggtgacct ggagccgagg
      841 cccacggctc ctagctccgg cgcgcgcgcc aggatcgacc actaccggca cacagccgcg
      901 cagctcggcg agaagctgct ccggcctagc ctggccgagc tgcatgacga gctggaaaag
      961 gaaccttttg aggatggctt tgcaaatggg gaagaaagca ctccaacacg tgatgctgtg
     1021 gtcacgtaca cggccgaaag taaaggagtc gtgaagtttg gctggatcaa gggtgtacta
     1081 gtacgttgta tgttaaacat ttggggtgtg atgcttttca ttcgtctctc atggattgtg
     1141 ggacaagctg gaattggtct atcagtcctt gtaataatga tggctactgt tgtgacaact
     1201 atcacaggat tgtccactag cgccatcgca actaatggat ttgtaagggg aggcggcgca
     1261 tattatttaa tatctagaag tctagggcct gaatttggtg gtgccattgg tctaatcttc
     1321 gcctttgcca acgctgttgc tgttgctatg tatgtggttg gatttgcaga aaccgtggtg
     1381 gagttgctga aagaacattc cattcttatg atagatgaaa tcaatgacat ccggattatt
     1441 ggagccatta cagtcgtgat tcttttaggt atctcagtag ccggtatgga gtgggaagca
     1501 aaagctcaga ttgttctttt ggtgatccta cttcttgcta ttggtgattt cgtcatagga
     1561 acatttatcc cactcgagag caagaagcca aaagggtttt ttggctataa atctgaaatc
     1621 tttaatgaga actttggccc cgattttcga gaagaagaaa ctttcttttc tgtatttgcc
     1681 atcttttttc ctgctgcaac tggaattctg gctggcgcaa atatctcagg tgatcttgca
     1741 gatcctcagt cagccatacc caaaggaaca ctcctagcca ttttaattac tacattggtt
     1801 tatgtaggaa ttgccgtgtc tgtaggttct tgtgtcgtac gcgatgccac tggtaatgtt
     1861 aatgacacta tcgtaacaga gctaacaaac tgtactagcg cagcctgcaa attaaacttt
     1921 gatttttcat cttgtgaatc cagtccttgt agctacggcc taatgaacaa ctttcaggta
     1981 atgagtatgg tgtcaggatt tacaccacta atttccgcgg gcatattctc tgccactctt
     2041 tctagcgcat tagcatccct agtgagtgct cccaagatat ttcaggccct atgtaaagac
     2101 aacatctacc ccgctttcca gatgtttgct aaaggttatg ggaaaaataa tgagccatta
     2161 cgtggctaca tcttaacatt cttaattgca cttggattca tcttaattgc cgaactgaat
     2221 gttattgcat gtattatctc aaacttcttc cttgccagct atgccctcat caatttttca
     2281 gtattccatg cttcactggc caaaagccca ggatggcgac cggcatttaa atactacaat
     2341 atgtggatct cacttcttgg agcaattctt tgttgcatag taatgttcgt ctgtaactgg
     2401 tgggctgctt tgctaaccta cgtgatagtc cttggtctct atatttatgt tacctacaaa
     2461 aaacccgatg tgaattgggg atcctctaca caagccctga cttacctcaa tgccctgcaa
     2521 catagtatta ggctttctgg cgtggaggac catgtgaaaa actttaggcc acagtgtctt
     2581 gttatgacag gagcgccaaa ctcacggccg gccttacttc atcttgttca cgatttcaca
     2641 aaaaatgttg gtttgatgat ctgtggacac gtacacatgg gaccacgcag acaagccatg
     2701 aaagaaatgt ccatcgacca agccaaatat cagcgctggc ttattaagaa caaaatgaag
     2761 gctttttatg caccagtaca tgcagatgac ctcagagaag gggcccaata cttgatgcag
     2821 gctgctggcc tgggccgtat gaagccaaac acactagtcc ttggatttaa gaaagattgg
     2881 ttgcaagcag atatgaggga tgtggatatg tacataaact tatttcacga tgcttttgac
     2941 atacaatatg gcgtagtggt tattcgccta aaagaaggtc tggatattag tcacctgcag
     3001 ggacaagaag aattattgtc atcacaagag aaatctcctg gcaccaagga tgtcgtagta
     3061 agtgtggaat atagtaaaaa gtccgattta gatacttcga aacccctcag tgaaaaacca
     3121 attacccata aagtcgagga agaagatggc aagactgcaa ctcaaccact gttgaaaaaa
     3181 gaatccaaag gccctattgt gcctttgaat gtagctgacc aaaagcttct tgaagctagt
     3241 acacagtttc agaaaaaaca aggaaagaat actattgatg tctggtggct ttttgatgac
     3301 ggaggtctga ccttattgat accttacctt ctgacgacca agaaaaaatg gaaagactgt
     3361 aagatcagag tattcattgg tggaaagata aacagaatag accatgaccg gagagcgatg
     3421 gctactttac tgagcaagtt cagaatcgat ttttctgaca tcatggttct aggagacatc
     3481 aataccaaac caaagaaaga aaacattata gctttcgagg aaatcattga gccatacaga
     3541 cttcacgaag atgataaaga gcaagatatc gcagataaaa tgaaagaaga tgaaccctgg
     3601 cgaataacag ataatgaatt ggagctgtat aagaccaaga cataccggca gatcaggtta
     3661 aatgagttat taaaggaaca ttcaagcaca gctaacatta ttgtgatgag tttaccggtc
     3721 gcacgaaagg gtgccgtttc tagtgctctc tacatggcat ggttagaagc actatctaag
     3781 gatctaccac caatcctact agttcgtggg aatcatcaga gtgtccttac cttctattca
     3841 taattaatta agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     3901 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     3961 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     4021 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     4081 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     4141 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4201 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     4261 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4321 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     4381 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     4441 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     4501 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     4561 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     4621 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     4681 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4741 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     4801 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     4861 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     4921 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt
     4981 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     5041 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     5101 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     5161 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     5221 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     5281 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     5341 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     5401 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     5461 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     5521 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     5581 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     5641 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     5701 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     5761 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     5821 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     5881 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     5941 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     6001 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     6061 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     6121 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6181 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6241 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     6301 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     6361 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     6421 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6481 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6541 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6601 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6661 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     6721 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6781 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6841 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6901 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6961 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7021 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtttttttgt ttgcaagcag
     7081 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7141 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     7201 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     7261 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7321 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7381 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7441 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7501 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7561 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7621 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7681 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7741 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7801 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     7861 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     7921 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     7981 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8041 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8101 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8161 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     8221 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
     8281 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
     8341 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
     8401 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
     8461 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
     8521 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     8581 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     8641 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     8701 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     8761 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     8821 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     8881 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     8941 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     9001 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     9061 ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg
     9121 cttactggct tatcgaaatt aatacgactc actataggga gacccaagct g
//
